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Anti myb

Manufactured by Abcam
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Anti-Myb is a laboratory reagent used in research applications. It is an antibody specific to the Myb protein, which plays a role in cell proliferation and differentiation. This product can be used for applications such as Western blotting, immunohistochemistry, and immunoprecipitation to detect and study the Myb protein.

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Lab products found in correlation

Anti twist, by Abcam (1 mentions) Anti zeb1, by Abcam (1 mentions) Anti p stat3, by Cell Signaling Technology (1 mentions) Anti p src, by Cell Signaling Technology (1 mentions) Anti t stat3, by Cell Signaling Technology (1 mentions) Anti e cad, by Abcam (1 mentions) Anti vim, by Abcam (1 mentions) Anti n cad, by Abcam (1 mentions) Anti nedd9, by Abcam (1 mentions) Anti t src, by Cell Signaling Technology (1 mentions) Anti snail, by Abcam (1 mentions) Anti bcl 2, by Abcam (1 mentions) Anti gapdh, by Abcam (1 mentions) Anti bax, by Abcam (1 mentions) Anti pcna, by Abcam (1 mentions) Radioimmunoprecipitation assay lysis buffer, by Thermo Fisher Scientific (1 mentions) Iblot dry blotting system, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit hrp, by Cell Signaling Technology (1 mentions) Detergent compatible protein assay, by Bio-Rad (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Human anti-C-myb antibody (5 citations) Anti-c-myb (4 citations) Anti-MYB antibody (3 citations) Rabbit anti-MYB antibody (2 citations)

4 protocols using anti myb

1

EMT Regulation and Src/STAT3 Signaling

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The study protocol was performed according to the principles of the Declaration of Helsinki and the ethics committee of the Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China. Informed consent was obtained from all patients.
The following primary antibodies were purchased from Abcam (Cambridge, England): anti-E-cad, anti-Vim, anti-Snail, anti-N-cad, anti-Twist, anti-PCNA, anti-Bax, anti-Bcl-2, anti-ZEB1, anti-NEDD9, anti-Myb, and anti-GAPDH. The following primary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA): anti-t-SRC, anti-p-SRC, anti-t-STAT3, and anti-p-STAT3. PP1, an SRC inhibitor, was purchased from Selleck Chemicals (Houston, TX, USA).
Xue Y., Wu T., Sheng Y., Zhong Y., Hu B, & Bao C. (2021). MicroRNA-1252-5p, regulated by Myb, inhibits invasion and epithelial-mesenchymal transition of pancreatic cancer cells by targeting NEDD9. Aging (Albany NY), 13(14), 18924-18945.
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2

Western Blot Analysis of Primagraft Proteins

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ACC x11 (from long-term tumor growth inhibition studies) and ACC x5M1 (from short-term kinetics studies) primagrafts were mechanically homogenized in radioimmunoprecipitation assay lysis buffer (Thermo Scientific) supplemented by the addition of complete protease inhibitor cocktail (Roche). Protein concentrations were determined by detergent compatible protein assay (Bio-Rad). Samples were denatured by adding Laemmli sample buffer (Bio-Rad) with β-mercaptoethanol (Sigma) and boiled at 95°C for 5 min. 10 µg of protein was resolved by electrophoresis through 4–20% mini-PROTEAN TGX (Bio-Rad) precast gels. Proteins were transferred onto a nitrocellulose membrane using the iBlot dry blotting system (Invitrogen). Membranes were incubated overnight with the following primary antibodies: anti-Myb (ab45150; Abcam), anti-RARα (MAB5346; Chemicon), and anti-GAPDH (2118s; Cell Signaling Technology) as loading control. Protein bands were detected using horse anti-mouse HRP (7076S; Cell Signaling Technology) or goat anti-rabbit HRP (7074S; Cell Signaling Technology). Three biological replicates (individual primagrafts from different mice) for each treatment group were analyzed for protein expression. HEK293T cell lysate, which lacks MYB expression, was processed similarly and used as a negative control on the same blots.
Mandelbaum J., Shestopalov I.A., Henderson R.E., Chau N.G., Knoechel B., Wick M.J, & Zon L.I. (2018). Zebrafish blastomere screen identifies retinoic acid suppression of MYB in adenoid cystic carcinoma. The Journal of Experimental Medicine, 215(10), 2673-2685.
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3

Immunophenotyping of Cell Monolayers

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Monolayer of asynchronous cells in logarithmic growth phase were fed with fresh complete medium two hours before harvesting, then trypsinized and suspended in cold 1×PBS solution. An approximate 1× 105 cells were cytocentrifuged (Shandon Scientific) on to glass slides and fixed in acetone for 10 min, then air dried. Multiple primary antibodies were used for staining: a) Lineage-related Markers: Cytokeratin Cocktail (Dako), CAM 5.2 (BD Bioscientifics) and anti-α-SMA (Sigma-Aldrich). b) Biomarkers: Cytospin preparation of acetone fixed cells were prepared and stained with Anti-EGFR (Life Technologies-Dako, CA), Anti-c-Met (Ventana, CA), Anti-c-Kit (Dako), Anti-p63 (Santa Cruz) and Anti- Myb (Abcam) antibodies. Slides were processed for immunostaining using the DAKO Autostainer.
Li J., Perlaky L., Rao P., Weber R.S, & El-Naggary A.K. (2014). Development and Characterization of Salivary Adenoid Cystic Carcinoma Cell Line. Oral oncology, 50(10), 991-999.
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4

Protein Expression Analysis of MDR Factors

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Total cellular protein was extracted using ice-cold RIPA lysis buffer (50 mM Tris–HCl, pH = 7.4, 150 mM NaCl, 0.1% SDS, 1% Triton X-100, 0.5% sodium deoxycholate) containing 1× complete protease inhibitor cocktail (EDTA-free, Sigma-Aldrich). Protein extracts were electrophoresed on a 10% SDS polyacrylamide gel and transferred to the Immobilon-P PVDF membrane (Millipore, Billerica, MA, USA). The following primary antibodies were used: anti-multidrug resistance-related protein 1 (anti-MRP1, Abcam, Cambridge, UK; dilution 1 : 1000), anti-multidrug resistance 1 (anti-MDR1, Abcam; dilution 1 : 2000), anti-Myb (Abcam; dilution 1 : 1000) and anti-β-actin (Abcam; dilution 1 : 5000). Horseradish peroxidase-conjugated IgG (Abcam; dilution 1 : 5000) was used a second antibody. Protein bands were detected using ECL reagent (GE Healthcare Technologies, Waukesha, WI, USA) and analyzed by ImageJ software (National Institutes of Health, Bethesda, MD, USA).
Li Q, & Wang J. (2019). Long noncoding RNA ZFAS1 enhances adriamycin resistance in pediatric acute myeloid leukemia through the miR-195/Myb axis. RSC Advances, 9(48), 28126-28134.
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