All tissue samples were obtained from patients treated at The First Affiliated Hospital of Zhengzhou University. Each patient signed an informed consent form, and this study was approved by the First Affiliated Hospital of Zhengzhou University Institutional Review Board. Tissues samples were collected from OC patients with unilateral ovarian invasion and normal contralateral ovary, including 17 cases of high-grade serous carcinoma, 2 cases of mucinous cystadenoma and 1 case of mucinous carcinoma. Human OC cell lines (A2780, SKOV3, OVCAR3) and a normal ovary cell line (IOSE80) were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). All cell lines were authenticated using the short tandem repeat (STR) profiling test. Then, IOSE80, A2780, OVCAR3 cells were cultured in RPMI-1640 Medium (Biological Industries, USA) supplemented with 10% fetal bovine serum (FBS, Biological Industries, USA), 100 units/ml penicillin, and 100 μg/ml streptomycin (Biological Industries, USA). SKOV3 cell was maintained in McCoy’s 5A Medium (Biological Industries, USA) supplemented with 10% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin.
Iose80
Manufactured by Sartorius
Sourced in United States
The IOSE80 is a laboratory equipment product offered by Sartorius. It is designed to provide a core function for laboratory applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further details on the intended use or specific features of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Sartorius (1 mentions)
Penicillin, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Mccoy s 5a medium, by Sartorius (1 mentions)
A2780, by Sartorius (1 mentions)
Ovcar3, by Sartorius (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Ovcar3, by Thermo Fisher Scientific (1 mentions)
Ovcar3, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
A2780, by Thermo Fisher Scientific (1 mentions)
A2780, by BNCC (1 mentions)
Rpmi 1640, by Sartorius (1 mentions)
Hey t30, by American Type Culture Collection (1 mentions)
Skov3, by Sartorius (1 mentions)
2 protocols using iose80
1
Ovarian Cancer Tissue Samples and Cell Lines
2
Ovarian Cell Lines Culture Conditions
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The normal ovarian cell line (IOSE-80) was obtained from Shanghai Yaji Biotechnology Co., Ltd. The human ovarian cancer cell lines (ES-2, SKOV3, OVCAR3 and HEY-T30) were purchased from American Type Culture Collection. A2780 cells were purchased from BeNa Culture Collection (Beijing Beina Chunglian Biotechnology Research Institute). IOSE-80, ES-2, HEY-T30 and SKOV3 cells were cultured in RPMI-1640 (Biological Industries) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin. OVCAR3 and A2780 cells were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were cultured at 37°C in a 5% CO2 incubator.
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