Data were analysed using Microsoft Office Excel 2017 and GraphPad Prism 8.0. Data are expressed as mean ± standard error of the mean (SEM). Comparisons of means were analysed by one-way analysis of variance (ANOVA). Dunnett’s post-hoc test was used for multiple comparisons, with beef set as the control group. Differences were significant when p < 0.05.
Excel 2017
Manufactured by GraphPad
Excel 2017 is a spreadsheet software application that allows users to organize, analyze, and visualize data. It provides a range of features for creating and managing worksheets, formulas, and charts. Excel 2017 is part of the Microsoft Office suite of productivity tools.
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Lab products found in correlation
7 protocols using excel 2017
1
Comparative Analysis of Beef Samples
2
Coculture Statistical Analysis Protocol
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Data are presented generally as mean ± standard error of the mean. Microsoft Excel 2017 and Graphpad Prism 7 (plotting and statistical testing) were used to analyse the data by one‐way analysis of variance with the Dunnett's post hoc test for comparison with controls if not otherwise indicated in the figures. Significance levels: ***p < .001, **p < .01, *p < .05, or n.s.: not significant. For expression data, ΔCq values were analysed for significance and transformed to 2−ΔCq. For all statistical tests, data from all cocultures from all mice inside an experiment were pooled.
3
Statistical Analysis of Experimental Data
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All data were statistically analyzed using Microsoft Excel 2017 software and Graph pad Prism 5 software. Results are presented as means ± standard deviation (SD) of three independent experiments. The statistical significance was determined by an unpaired Student’s t-test. All statistical significances were considered when a p-value was less than 0.05.
4
ACE2 Expression Analysis in Human Brain
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Datasets were independently searched and analyzed by two authors (RC and JY), and any disagreements were discussed and resolved by consensus with the corresponding author (ZX). The data from the Allen Human Brain Atlas database and Allen Cell Types database were exported to Microsoft Excel 2017 and GraphPad Prism 6.0 for further analysis. ACE2 expression data from the Allen Human and Mouse Brain Atlas databases were imported to Brain Explorer 2.0 software to get visualization. The data from the other databases were analyzed online. Where applicable, data are expressed as median or mean. Interquartile range (IQR), 95% CI, range, and/or all sample points were also provided if possible.
Positive of expression criterion: (1) to minimize false positive of expression as previous studies (32 (link), 33 (link)), a gene with calculate counts per million (CPM), transcripts per million (TPM), unique molecular identifier (UMI) count, or fragments per kilobase million (FPKM) >1 were considered to be positive, which also is the same as log10 (CPM), log10 (TPM), or log10 (UMI) ≥0 as well as log10 (CPM + 1), log10 (TPM + 1), and log10 (UMI + 1) ≥0.3; (2) besides, as a z score being >2 corresponds to a p-value <0.05, a z score of ACE2 expression >2 was considered as high ACE2 expression in the Allen Human Brain Atlas database.
Positive of expression criterion: (1) to minimize false positive of expression as previous studies (32 (link), 33 (link)), a gene with calculate counts per million (CPM), transcripts per million (TPM), unique molecular identifier (UMI) count, or fragments per kilobase million (FPKM) >1 were considered to be positive, which also is the same as log10 (CPM), log10 (TPM), or log10 (UMI) ≥0 as well as log10 (CPM + 1), log10 (TPM + 1), and log10 (UMI + 1) ≥0.3; (2) besides, as a z score being >2 corresponds to a p-value <0.05, a z score of ACE2 expression >2 was considered as high ACE2 expression in the Allen Human Brain Atlas database.
5
Statistical Analysis of Experimental Data
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Data were expressed as mean ± SD. Significant differences were assessed by two-tailed Student’s test. Two-sided P-value <0.05 was considered statistically significant. Analyses were performed with Excel 2017 and GraphPad Prism 8.0.
6
Comprehensive Statistical Analysis Protocol
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All statistics underlying the graphs and charts were analyzed by Excel 2017 and Prism (version 5, GraphPad). All experiments were independently performed in triplicate at least, indicated in the corresponding figure legend. Images were combined and annotated in Adobe Photoshop (version 2019).
7
Statistical Analysis of Experimental Data
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We used Excel 2017 software for the data statistics, and the data drawing application Graphpad Prism 8.4.3. We used SPSS 21.0 (IBMCorp., Armonk, NY, USA) software to analyze the significant differences in the data based on the one-way analysis of variance (ANOVA) and Duncan methods, and the significant difference level was p < 0.05.
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