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Matrigel coated transwell chambers with 8.0 m pet membranes

Manufactured by Corning
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Matrigel-coated transwell chambers with 8.0 µm PET membranes are a type of lab equipment used for cell culture and migration studies. The transwell chambers have a porous polyethylene terephthalate (PET) membrane with a pore size of 8.0 micrometers. The membrane is coated with Matrigel, a basement membrane extract derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. This setup allows for the study of cell migration and invasion through a barrier.

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Lab products found in correlation

Sodium hyaluronate, by Lifecore Biomedical (2 mentions)

Close spelling variants of this product

8.0 µm PET Membrane Matrigel-coated Transwell chambers Transwell chamber with matrigel 8 µm transwell chamber PET Transwell membranes Matrigel Transwell chambers Matrigel-coated chambers Matrigel-coated membrane Coated transwell chambers Matrigel-coated Transwell

2 protocols using matrigel coated transwell chambers with 8.0 m pet membranes

1

Transwell Invasion Assay with Hyaluronic Acid

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Cellular invasion was quantified using the transwell invasion assay as previously reported (30 (link)). Briefly, Matrigel-coated transwell chambers with 8.0 µm PET membranes (Corning, Cat# 354483) were rehydrated with serum-free media before being seeded with 10,000 cells per chamber. Cells were then allowed to invade towards media containing serum for 48 hours. For hyaluronic acid rescue studies, sodium hyaluronate (Lifecore Biomedical, Cat # HA15M-1) was resuspended in media at 4 mg/ml. Experimental samples were performed in triplicate and pooled.
Arnold J.M., Gu F., Ambati C.R., Rasaily U., Ramirez-Pena E., Joseph R., Manikkam M., Martin R.S., Charles C., Pan Y., Chatterjee S.S., Hollander P.D., Nagi C., Sikora A.G., Rowley D., Putluri N., Karanam B., Mani S.A, & Sreekumar A. (2019). UDP-Glucose 6-Dehydrogenase regulates hyaluronic acid production and promotes breast cancer progression. Oncogene.
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2

Transwell Invasion Assay with Hyaluronic Acid

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular invasion was quantified using the transwell invasion assay as previously reported (30 (link)). Briefly, Matrigel-coated transwell chambers with 8.0 µm PET membranes (Corning, Cat# 354483) were rehydrated with serum-free media before being seeded with 10,000 cells per chamber. Cells were then allowed to invade towards media containing serum for 48 hours. For hyaluronic acid rescue studies, sodium hyaluronate (Lifecore Biomedical, Cat # HA15M-1) was resuspended in media at 4 mg/ml. Experimental samples were performed in triplicate and pooled.
Arnold J.M., Gu F., Ambati C.R., Rasaily U., Ramirez-Pena E., Joseph R., Manikkam M., Martin R.S., Charles C., Pan Y., Chatterjee S.S., Hollander P.D., Nagi C., Sikora A.G., Rowley D., Putluri N., Karanam B., Mani S.A, & Sreekumar A. (2019). UDP-Glucose 6-Dehydrogenase regulates hyaluronic acid production and promotes breast cancer progression. Oncogene.
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