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5 protocols using j750 polyjet printer

1

Additive Manufacturing of Biomedical Devices

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Materials for the Stratasys J750 PolyJet printer (VeroClear, MED610, and Tango+) were obtained directly from Stratasys (Eden Prairie, MN). ATP, crude firefly lanterns, HBSS, tris (hydroxymethyl)-aminomethane, CaCl2, NaCl, Mg(SO4), glucose, and glycerol were obtained from Sigma-Aldrich (St. Louis, MO). Luciferin was obtained from GoldBiotechnology (St. Louis, MO). KCl was obtained from Fisher Scientific (Fair Lawn, NJ). Bovine serum albumin was obtained from MP Biomedical.
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2

Multi-Material 3D Printing for Prototypes

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All finalized designs were printed on the Stratasys J750 PolyJet printer using the software GrabCad (Stratasys, Cambridge, MA). This printer is capable of multi-material photopolymer printing allowing for designation of shore value selection and filament color selection. It prints on the resolution of 42/42/14 (h/l/w) microns and carries a build plate dimension of 490×390×200mm. The printer supports water and alcohol soluble support materials which require prints to go through post processing while they are done. Finalized prints are submerged in 99% Isopropyl Alcohol until support materials are dissolved and washed off with a waterjet allowing for smooth finish of cavity prints.
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3

Fabrication of Luneburg Lenses

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The 40 kHz two-dimensional Luneburg lens was fabricated with a Stratasys J750 PolyJet printer. The printed sample was embedded in a jelly-like support material and a high-pressure water splashing machine was utilized to remove the support material. The 8 kHz three-dimensional Luneburg lens was fabricated with a Form 2 SLA printer. The spherical design was divided into two hemispheres to eliminate the necessity of extensive supporting structures. The two independently printed hemispheres were jointed with a UV bonding process.
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4

Stratasys J750 PolyJet 3D Printing Materials

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3D-printer materials for the Stratasys J750 PolyJet printer were obtained directly from Stratasys (Eden Prairie, MN). Fluorescein, ATP, crude firefly lanterns, tris(hydroxymethyl)-aminomethane, CaCl2, NaCl, MgSO4, glucose, chloroplatinic acid, lead (II) acetate, Nafion, glybenclamide, L-NAME (N-Nitro-L-arginine methyl ester), D-mannitol, adenine, and phosphate buffered saline (10x) were obtained from Sigma-Aldrich (St. Louis, MO). Luciferin was obtained from Gold Biotechnology (St. Louis, MO). KCl was obtained from Fisher Scientific (Fair Lawn, NJ). Bovine serum albumin was obtained from MP Biomedical (Irvine, CA). Argon and NO gas were supplied by AirGas (Radnor, PA). The platinum and gold electrode materials were obtained from Alfa Aesar (Haverhill, MA). Oxyrase came from Oxyrase, Inc. (Mansfield, OH).
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5

Multi-Material 3D Printing for Prototypes

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All finalized designs were printed on the Stratasys J750 PolyJet printer using the software GrabCad (Stratasys, Cambridge, MA). This printer is capable of multi-material photopolymer printing allowing for designation of shore value selection and filament color selection. It prints on the resolution of 42/42/14 (h/l/w) microns and carries a build plate dimension of 490×390×200mm. The printer supports water and alcohol soluble support materials which require prints to go through post processing while they are done. Finalized prints are submerged in 99% Isopropyl Alcohol until support materials are dissolved and washed off with a waterjet allowing for smooth finish of cavity prints.
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