Anti mouse cd11c monoclonal antibody

Bone marrow–derived dendritic cells (BMDCs) isolated from murine femurs and tibias were cultured in plates as previously described [30 (link)]. Either RBD-PP or RBD was added to each well at 0.05 mg/ml, followed by incubation at 37 °C in 5% CO₂ for 4 h. The incubated BMDCs were stained with the anti-mouse CD11c monoclonal antibody (BioLegend) at 4 °C for 30 min. After the excess antibody was removed by centrifugation, BMDCs were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, blocked with 2% BSA (Sigma), incubated with rabbit anti-SARS-CoV-2-S1 monoclonal antibody (Sino Biological, 1:200 dilution) at 37 °C for 2 h, incubated with goat anti-rabbit IgG-FITC (Proteintech, 1:50 dilution) at 37 °C for 1 h, and finally stained with 4′,6-diamidino-2-phenylindole (DAPI) (Meilunbio). The stained BMDCs were imaged using an ImageXpress Micro Confocal System (Molecular Devices).