ELISA kits #CSB-E10684h and #CSB-E04740h (Cusabio, Houston, TX, USA) were used to assess Aβ42 and TNF-α plasma levels following the manufacturer’s instructions. Datasheets reported kit sensitivity of 0.078 ng/mL and 1.95 pg/mL for Aβ42 and TNFα, respectively. Values below the sensitivity of the kit were excluded from analysis.
Csb e10684h
Manufactured by Cusabio
Sourced in China
The CSB-E10684h is a laboratory equipment product. It is designed to perform a specific function in the laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Csbe12011h, by Cusabio (3 mentions)
Khb7041, by Thermo Fisher Scientific (2 mentions)
Kho0631, by Thermo Fisher Scientific (2 mentions)
Khb8051, by Thermo Fisher Scientific (2 mentions)
Khb7031, by Thermo Fisher Scientific (2 mentions)
P tau231t, by Thermo Fisher Scientific (2 mentions)
Csb e18033h, by Cusabio (2 mentions)
Csb e04740h, by Cusabio (1 mentions)
Csb e08299 h, by Cusabio (1 mentions)
6 protocols using csb e10684h
1
Quantifying Plasma Aβ42 and TNF-α
2
Glymphatic Efflux of Amyloid-beta Peptides
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These glymphatic efflux procedures were performed as previously described [41 (link)]. In brief, mice were anesthetized with 1% pentobarbital (i.p.) and placed in a stereotaxic frame. A mixture of human Aβ40 (0.0433 mg/L) and Aβ42 (0.0451 mg/L) peptides (Chinapeptides, China) was injected into the left prefrontal cortex (2 mm AP, 1 mm lateral, and 0.75 mm deep relative to the bregma). After an infusion at a rate of 0.05 µL/min for ten minutes, the needle was left in place for ten minutes prior to removal. Mice were sacrificed one hour after the end of the injection, and the left cerebral hemisphere was collected. For the Aβ analysis, tissues were first homogenized in 1 ml/g PBS and then assayed using human ELISA kits for Aβ40 (CSB-E08299h, Cusabio, China) and Aβ42 (CSB-E10684h, Cusabio, China).
3
Plasma Aβ42 and Tau Quantification
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Aβ42 and total tau levels in plasma were quantified using a commercially available ELISA kit from CUSABIO, China (CSB-E10684h, CSB-E12011h). The sandwich ELISA procedure was employed in these kits and the yellow color developed because of horse radish peroxidase reaction was read at 450 nm with a wavelength correction of 540 nm in a plate reader.
4
Quantification of Pathological Proteins in CSF
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The CSF levels of pathological proteins were determined using an enzyme-linked immunosorbent assay, including those of α-synuclein oligomer, Aβ1-42, T-tau, and tau phosphorylated at the following positions: threonine 181 (P-tau181t), threonine 231 (P-tau231t), serine 396 (P-tau396s), and serine 199 (P-tau199s). CSB-E18033h, CSB-E10684h, and CSB-E12011h kits for measuring α-synuclein oligomer, Aβ1-42, and T-tau, respectively, were obtained from CUSABIO (Wuhan, China), while KHB7031, KHB7041, KHB8051, and KHO0631 kits for measuring P-tau396s, P-tau199s, P-tau231t, and P-tau181t, respectively, were obtained from Invitrogen (Carlsbad, CA, USA). The levels of α-synuclein oligomer, Aβ1-42, T-tau, P-tau181t, P-tau231t, P-tau396s, and P-tau199s were measured using a quantitative sandwich enzyme immunoassay.
5
Quantification of Neuropathological Proteins in CSF
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The levels of neuropathological proteins in CSF from patients in PD-NPSs and PD-nNPSs groups were measured by using ELISA. CSB-E18033h, CSB-E10684h, and CSBE12011h (CUSABIO Company, Wuhan, China) were used for the measurements of α-synuclein, Aβ1 − 42, and T-tau, respectively. KHB7031 kit, KHB7041 kit, KhB8051 kit, and KHO0631 kit (Invitrogen Company, Carlsbad, America) were used for the measurements of P-tau (T181), P-tau (T231), P-tau (S396), and P-tau (S199), respectively.
6
Quantifying Pathological Proteins in PD
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The levels of pathological proteins, including Aβ1–42, T-tau, P-tau181t, P-tau396s and tau phosphorylated at the following positions: threonine 231 (P-tau231t) and serine 199 (P-tau199s), in CSF from PD patients and control participants were determined by using an enzyme-linked immunosorbent assay.
CSB-E10684h and CSBE12011h kits for measuring Aβ1–42 and T-tau, respectively, were obtained from CUSABIO (Wuhan, China). KHB7031, KHB7041, KHB8051 and KHO0631 kits for measuring P-tau396s, P-tau199s, P-tau231t and P-tau181t, respectively, were obtained from Invitrogen (Carlsbad, CA, USA).
CSB-E10684h and CSBE12011h kits for measuring Aβ1–42 and T-tau, respectively, were obtained from CUSABIO (Wuhan, China). KHB7031, KHB7041, KHB8051 and KHO0631 kits for measuring P-tau396s, P-tau199s, P-tau231t and P-tau181t, respectively, were obtained from Invitrogen (Carlsbad, CA, USA).
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