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Cell counting kit 8 (cck8)

Manufactured by Immunoway
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Sourced in United States

The Cell Counting Kit-8 is a colorimetric assay used for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. It employs a water-soluble tetrazolium salt that is reduced by cellular dehydrogenases to produce a colored formazan dye, which can be measured spectrophotometrically.

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Lab products found in correlation

Varioskan flash spectrophotometer, by Thermo Fisher Scientific (1 mentions)

3 protocols using cell counting kit 8 (cck8)

1

Cell Proliferation Monitoring Using CCK-8

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The Cell Counting Kit-8 (CCK-8, ImmunoWay Biotechnology Company, Plano, TX, USA) assay was used to monitor cell proliferation. In brief, the cells transfected with siRNA or plasmid were placed on 96-well plates and cultured for 24 h, 48 h, 72 h, and 96 h. Then, the OD450 value was detected using the Thermo Scientific Varioskan Flash spectrophotometer (Thermo Scientific, Finland).
Tang P., Wu Y., Zhu C., Li Q, & Liu S. (2023). Microdissecting the Hypoxia Landscape in Colon Cancer Reveals Three Distinct Subtypes and Their Potential Mechanism to Facilitate the Development of Cancer. Journal of Oncology, 2023, 9346621.
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2

Cell Proliferation Assay for ESCC

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Cell Counting Kit-8 (CCK8, ImmunoWay Biotechnology Company Plano, TX, USA) was used to detect the growth capacity of ESCC cells. Cells transfected with sh-circUBE4B or overexpression plasmids were inoculated into 96-well plates and the OD450 values were measured after 24, 48, 72, 96, and 120 h of incubation, respectively.
Lyu Y., Tan B., Li L., Liang R., Lei K., Wang K., Wu D., Lin H, & Wang M. (2023). A novel protein encoded by circUBE4B promotes progression of esophageal squamous cell carcinoma by augmenting MAPK/ERK signaling. Cell Death & Disease, 14(6), 346.
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3

Cell Proliferation Quantification by CCK-8

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Cell Counting Kit-8 (CCK-8, ImmunoWay Biotechnology Company Plano, TX, USA) were performed to monitor the growth of cells. In brief, cells transfected with si-circFNDC3B or overexpression vectors were seeded into 96-well plates and cultured for 24, 48, 72 and 96 h, respectively. OD450 values were determined.
Pan Z., Cai J., Lin J., Zhou H., Peng J., Liang J., Xia L., Yin Q., Zou B., Zheng J., Qiao L, & Zhang L. (2020). A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer. Molecular Cancer, 19, 71.
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