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Antibody for bcl2

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Antibody for Bcl2 is a laboratory reagent designed to detect the presence and measure the levels of the Bcl2 protein. Bcl2 is a key regulator of apoptosis, or programmed cell death, and plays a crucial role in various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to study the expression and localization of Bcl2 in biological samples.

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2 protocols using antibody for bcl2

1

Molecular Mechanisms of Cytotoxicity

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Convallatoxin was obtained from Pfaltz & bauer (Waterbury, CT, USA). Peruvoside was purchased from Research Plus, Inc. (Barnegat, NJ, USA). Strophanthidin, Thiazolyl blue tetrazolium bromide (MTT), Cisplatin, Sodium dichromate (Na2Cr2O7.2H2O) [Cr(VI)], Triton X-100, DNAse free RNAse and Propidium Iodide were obtained from Sigma Aldrich (St Louis, MO, USA). Antibodies for p53, phospho-p53, CDK4, Cyclin D1, PARP, XIAP, p-62, phospho-Akt (Ser 473), total-Akt, phospho-ERK, total-ERK, pEGFR, EGFR, β-Catenin, Vimentin, Slug and peroxidase-labeled secondary rabbit and mouse antibodies were obtained from Cell Signaling Technology (Denvers, MA, USA). Antibody for Bcl2 was procured from Santa Cruz Biotechnology (Dallas, TX, USA). Bicinchoninic acid and Supersignal West Pico chemiluminescent substrate was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
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2

Evaluation of Cellular Stress Responses

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Thiazolyl blue tetrazolium bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)), cisplatin, sodium dichromate (Na 2 Cr 2 O 7 .2H 2 O) (Cr(VI)), Triton X-100, DNAse-free RNAse, and propidium iodide were obtained from Sigma Aldrich (St. Louis, MO). The oxidative probes 4,5-diaminofluorescein diacetate (DAF-2DA), dichlorofluorescein (DCF) diacetate, dihydroethidium (DHE), and the apoptosis dye Hoechst 33342 were obtained from Molecular Probes (Eugene, OR). Antibodies for PARP, caspase-9, p-Akt (Ser 473), total-Akt, p27, cyclin D3, cyclin-dependent kinase 2 (CDK2), p21, nuclear factor kappa B (NF-κB), and IkappaB kinase-beta (IKK-β) and peroxidase-labeled secondary rabbit and mouse antibodies were purchased from Cell Signaling Technology (Denvers, MA). Antibody for Bcl-2 was procured from Santa Cruz Biotechnology (Dallas, TX). Bicinchoninic acid (BCA) and SuperSignal ® West Pico chemiluminescent substrate were purchased from Thermo Fisher Scientific (Waltham, MA).
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