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Sodium acetate anhydrous

Manufactured by Avantor
Sourced in Poland

Sodium acetate anhydrous is a chemical compound used as a laboratory reagent. It is a white crystalline solid that is soluble in water and various organic solvents. Sodium acetate anhydrous is commonly used in a variety of laboratory applications, such as pH adjustment, buffer preparation, and as a source of acetate ions.

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6 protocols using sodium acetate anhydrous

1

Synthesis of Functional Polymer Materials

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Hydroxypropyl methacrylate (HPMA), 2-diethylamino ethyl methacrylate (DEAEMA), (2-(methacryloyloxy)ethyl) trimethylammonium chloride solution (TMAEMA), and N-(3-(dimethylamino)propyl) methacrylamide (DMAPMA) were purchased from Sigma-Aldrich; methyl methacrylate (MMA) and 3-sulfopropyl methacrylate potassium salt (SPMA) from VWR; butyl methacrylate (BMA) from Alfa Aesar; and poly(ethylene glycol) (n) monomethyl ether monomethacrylate (PEGMA, Mn ≈ 400 g mol−1) from Polysciences. PEGMA was deinhibited prior to use by passing over mono-methyl ether hydroxyquinone inhibitor removal resin. Ethyl 2-(phenylcarbonothioylthio)-2-phenylacetate, 4-nitrophenyl butyrate (PNB), hydrogen peroxide (H2O2), D-(+)-glucose, sodium acetate, lithium bromide were purchased from Sigma-Aldrich; zinc tetraphenyl porphyrin (ZnTPP), dimethyl sulfoxide (DMSO), 3,3,5,5-tetramethylbenzidine (TMB) from Fisher Scientific; and potassium phosphate (mono and dibasic) and sodium acetate anhydrous from VWR.
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2

Synthesis and Characterization of Cationic Polymer Brushes

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The monomers utilized were methyl methacrylate (MMA) from VWR, poly(ethylene glycol) (n) monomethyl ether monomethacrylate (PEGMA, Mn = 400 Da) from Polysciences, and [2-(methacryloyloxy)ethyl] trimethylammonium chloride solution (TMAEMA) and 3-sulfopropyl methacrylate potassium (SPMA) from Sigma-Aldrich. The PET-RAFT initiator zinc tetraphenyl porphyrin (ZnTPP) was purchased from Fisher Scientific while the chain transfer agent (CTA) ethyl 2-(phenylcarbonothioylthio)-2-phenylacetate was purchased from Sigma-Aldrich. Sodium acetate buffer (NaOAc) used in each characterization experiment was made with sodium acetate anhydrous from VWR. Poly(ethylene-co-acrylic acid) (PEAA) used to immobilize HRP for some QCMD experiments was purchased from Sigma-Aldrich.
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3

Synthesis of Functional Polymer Materials

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Hydroxypropyl methacrylate (HPMA), 2-diethylamino ethyl methacrylate (DEAEMA), (2-(methacryloyloxy)ethyl) trimethylammonium chloride solution (TMAEMA), and N-(3-(dimethylamino)propyl) methacrylamide (DMAPMA) were purchased from Sigma-Aldrich; methyl methacrylate (MMA) and 3-sulfopropyl methacrylate potassium salt (SPMA) from VWR; butyl methacrylate (BMA) from Alfa Aesar; and poly(ethylene glycol) (n) monomethyl ether monomethacrylate (PEGMA, Mn ≈ 400 g mol−1) from Polysciences. PEGMA was deinhibited prior to use by passing over mono-methyl ether hydroxyquinone inhibitor removal resin. Ethyl 2-(phenylcarbonothioylthio)-2-phenylacetate, 4-nitrophenyl butyrate (PNB), hydrogen peroxide (H2O2), D-(+)-glucose, sodium acetate, lithium bromide were purchased from Sigma-Aldrich; zinc tetraphenyl porphyrin (ZnTPP), dimethyl sulfoxide (DMSO), 3,3,5,5-tetramethylbenzidine (TMB) from Fisher Scientific; and potassium phosphate (mono and dibasic) and sodium acetate anhydrous from VWR.
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4

Modular Synthetic Polymer Synthesis

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The monomers utilized were methyl methacrylate (MMA) from VWR, poly(ethylene glycol) (n) monomethyl ether monomethacrylate (PEGMA, Mn = 400 Da) from Polysciences, and [2‐(methacryloyloxy)ethyl] trimethylammonium chloride solution (TMAEMA) and 3‐sulfopropyl methacrylate potassium (SPMA) from Sigma‐Aldrich. The PET‐RAFT initiator zinc tetraphenyl porphyrin (ZnTPP) was purchased from Fisher Scientific while the chain transfer agent (CTA) ethyl 2‐(phenylcarbonothioylthio)‐2‐phenylacetate was purchased from Sigma‐Aldrich. Sodium acetate buffer (NaOAc) used in each characterization experiment was made with sodium acetate anhydrous from VWR. Poly(ethylene‐co‐acrylic acid) (PEAA) used to immobilize HRP for some QCMD experiments was purchased from Sigma‐Aldrich.
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5

Collagen-based Wound Dressing Formulation

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Collagen (col) type I was obtained in our laboratory from fish scales of Esox lucius [38 (link)]. Gelatin type A (gel), hydroxyethyl cellulose (hec), Span 85, 1-ethyl-3(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), Folin-Ciocalteu reagent, and gallic acid were purchased from Sigma-Aldrich (Poznan, Poland). Hydrochloric acid, acetic acid, sodium biphosphate anhydrous, sodium phosphate, sodium acetate anhydrous, and sodium chloride were obtained from Avantor Performance Materials Poland S.A. (Gliwice, Poland). Ethyl alcohol, paraffin oil, disodium edetate dihydrate, and sodium carbonate were supplied by the Stanlab (Lublin, Poland). The hydroglycolic Calendula officinalis flower extract was acquired from Provital S.A. (Barcelona, Spain).
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6

Mycotoxin Extraction and Quantification

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The following reagents were used in the study: solventsmethanol, acetonitrile, n-hexane for HPLC, formic acid (LC-MS), ethyl acetate (Avantor Performance Materials Poland S.A., Gliwice), deionized water (Milli-Q Reagent Water (< 10 MΩ cm -1 resistivity) (Merck, Millipore); sorbents-Bondesil PSA, 40 μm (Labstore, Poland), Bakerbond octadecyl (C18, 40 μm, 60 Å), Bakerbond aminopropyl (NH 2 , 40 μm, 60 Å), Bakerbond 1°,2°-Amino (NH 2 /NH, 40 μm, 60 Å) from J.T Baker (Avantor Performance Materials Poland); salts-magnesium sulfate anhydrous (99.5% purity), sodium chloride (99.9% purity) (Avantor Performance Materials Poland), sodium citrate monobasic-HOC(COONa)(CH 2 COOH) 2 (99.5% purity), (Sigma-Aldrich, Poland), sodium acetate anhydrous (99.9% purity) (J.T. Baker, Avantor Performance Materials Poland); standards for ZEA (98% purity) and its metabolites-α-ZEL (98% purity), α-ZAL (97% purity), β-ZAL (98% purity), β-ZEL (98% purity); and an internal standard-zearalanone (ZAN: 98% purity) (Sigma-Aldrich, Poland).
The concentrations of standard solutions were 1152 ng/mL ZEA, 960 ng/mL α-ZAL and α-ZEL, and 800 ng/mL β-ZAL and β-ZEL. Solutions with lower concentrations were produced by diluting the working solutions to obtain concentrations of 0.4 to 748 ng/mL in the methanol/water (60:40, v/v) mobile phase.
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