After treating cells with different concentrations of AMTB (0 μM, 10 μM, 20 μM, 30 μM) for 24 h. Cell apoptosis was detected using Annexin V-FITC apoptosis detection kit (Boster Biological Technology Co., Ltd., California, USA) in accordance with the manufacturer’s instructions. Specimens were analyzed with BD FACS Calibur (Beckman Coulter, CA, USA) and the apoptotic rate was determined by the FlowJo software (Tree Star Inc., Ashland, USA). Each experiment was independently repeated for three times.
Annexin 5 fitc apoptosis detection kit
Manufactured by Boster Bio
Sourced in China
The Annexin V-FITC apoptosis detection kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule exposed on the surface of cells undergoing apoptosis. The Annexin V is labeled with the fluorescent dye FITC, allowing for the visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.
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3 protocols using annexin 5 fitc apoptosis detection kit
1
Apoptosis Assay of AMTB Treated Cells
2
Apoptosis Induction by T4O in PC Cells
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After 24 h, PC cells were seeded in six-well plates and treated with different concentrations (0, 0.5, 1, and 2 μM) of T4O. After washing three times with PBS, the cells were stained using the Annexin V-FITC Apoptosis Detection Kit (Boster, Wuhan, China) and then detected using a DeFLEX flow cytometer (Beckman, USA). The results were analyzed using Flwo JO.
3
Apoptosis Detection in HUVEC and MCF-7 Cells
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ERa-positive HUVEC and MCF-7 cells were separately seeded on 6-well plates with 4 Â 10 3 cells per well. After 24 h, cells were treated with formononetin for 48 h, collected, and then washed three times using ice-cold PBS. Cells were stained with Annexin Vfluorescein isothiocyanate (FITC) and propidium iodide (PI) for 30 min in the dark at room temperature in 500 mL of binding buffer. Under a FACS Aria flow cytometer (Becton Dickinson), nonstained cells were viable, and cells stained with Annexin V were judged at the early stage of apoptosis. Surface exposure of phosphatidylserine in apoptotic cells was measured using an Annexin V-FITC apoptosis detection kit (Boster, Hubei, China), according to the manufacturer's instructions. Additional exposure to PI made it possible to differentiate the early apoptosis cells
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