CS was performed using a Flexercell FX-4000T cell stretch system equipped with a 25-mm BioFlex Loading station (Flexcell International). The AT2 cells were mounted in the Strain Unit of the equipment, and then exposed to CS for 4 h (frequency, 0.5 Hz; time ratio of pull and relaxation, 1:1; amplitude, 20%; 30 cycles per min) to mimic stretch-induced AT2 cell injury (11 (link)). The AT2 cells in the sham group were also placed in the equipment and underwent the same process apart from the stretching.
25 mm bioflex loading station
Manufactured by Flexcell
The 25-mm BioFlex Loading station is a laboratory equipment designed to apply mechanical strain to cell cultures grown on BioFlex culture plates. It is a component of the BioFlex culture system.
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Lab products found in correlation
2 protocols using 25 mm bioflex loading station
1
Simulating Alveolar Cell Injury via Cyclic Stretch
2
High-Tidal Volume Mechanical Ventilation Assay
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CS experiments were performed using a Flexercell FX-4000T cell stretch system equipped with a 25-mm BioFlex loading station (Flexcell International). The cells (106) were mounted in a Flexercell FX-4000T Strain Unit and then exposed to 18% CS for 4 h (30 cycles per min) to recapitulate high-tidal volume mechanical ventilation regimen-induced cell injury30 (link). Control cells were also placed in the Flexercell Strain Unit and underwent all processes except stretch treatment. At the end of the experiment, the cells were collected for future analysis.
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