Sv30010

Human cancer cell lines, SW620, H358, and H293T, were purchased from ATCC, UT89 was generated from a primary recurrent uterine serous tumor in the laboratory of Dr. Analisa DiFeo. SW620 and H358 were cultured in RPMI, H293T and UT89 were cultured in DMEM. All media was supplemented with 10% FBS (ThermoFisher, SH3007003) and 1% penicillin/streptomycin (GE Healthcare, SV30010). HMECs were obtained from Dr. Mark Jackson. Specimen 48R and derivatives were grown as described previously^{21 ,22 (link)}. HMEC derivatives were generated as previously described^{21 ,23 (link)}. All cells were grown in a humidified atmosphere containing 5% CO₂. All cells lines underwent monthly testing for mycoplasma contamination (Lonza, LT07-710). AZD-6244 (Selleck Chemical, S1008) was dissolved in DMSO at a concentration of 80 mM and stored at −20°C (for in vitro studies). Okadaic Acid (OA) (P212121, LC-O-2220) was dissolved in DMSO at a concentration of 10 mM and stored at −20°C.

Human cell lines H358 and H293T, were purchased from ATCC and cultured in RPMI. All maintenance media was supplemented with 10% heat inactivated fetal bovine serum (FBS, ThermoFisher, SH3007003) and 1% penicillin/streptomycin (10,000 U/mL, GE Healthcare, SV30010), unless otherwise stated. All cells were cultured in a humidified atmosphere incubator containing 5% CO₂ at 37°C. All cell lines underwent monthly testing for mycoplasma contamination (Lonza, LT07–710).