Fluorescence and DIC images were acquired using a Nikon Ti Eclipse inverted microscope with a Nikon 100× Plan-Apo objective, NA 1.4, at 23°C. Images were captured with a CMOS camera (Xyla 4.2, Andor Technology) using NIS Elements imaging software and analyzed using ImageJ image analysis software (National Institutes of Health; https://imagej.net ). All images were acquired with exposure times between 50 and 500 ms, depending on the experiment and the channel of acquisition.
Xyla 4
Manufactured by Oxford Instruments
The Xyla 4.2 is a lab equipment product developed by Oxford Instruments. It is a measurement device designed to provide accurate and reliable data. The core function of the Xyla 4.2 is to perform precise measurements, but its intended use is not provided in this unbiased and factual description.
Automatically generated - may contain errors
2 protocols using xyla 4
1
Fluorescence and DIC Microscopy Imaging
2
Embryonic Heart Rate Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To analyze heart rate, embryos were anesthetized in 0.16 mg/mL tricaine and mounted in 1% low-melt agarose. Hearts were imaged on a Nikon Ti-2 SpectraX Widefield microscope with an Andor Xyla 4.2 megapixel, 16-bit sCMOS monochromatic camera at 200 frames per second (fps) in a controlled temperature chamber (28.5°C). Analysis performed in Nikon Elements software.
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