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Pdonr223 myc wt

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PDONR223_MYC_WT is a plasmid available through Addgene. This plasmid contains the MYC gene.

Automatically generated - may contain errors

Lab products found in correlation

Lr clonase, by Thermo Fisher Scientific (2 mentions) Puromycin, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PDONR223 (6 citations)

2 protocols using pdonr223 myc wt

1

Lentiviral Transduction of MYC in Leukemia

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The human MYC cDNA was purchased from Addgene (pDONR223_MYC_WT, a gift from Jesse Boehm and Matthew Meyerson and David Root (plasmid # 82927; http://n2t.net/addgene:82927; RRID: Addgene_82927). The MYC cDNA was cloned into the pCW57.1 vector (gift from David Root (Addgene plasmid # 41393; http://n2t.net/addgene:41393; RRID:Addgene_41393) using LR clonase (Thermo). The purified plasmid was used to generate lentivirus in HEK-293T cells, which was used to transduce MV4; 11 cells. A stable transduced polyclonal line was selected with 0.5 μg/mL puromycin (Thermo). The MYC cDNA was also cloned into pINDUCER21 (ORF-EG) which was a gift from Stephen Elledge and Thomas Westbrook (Addgene plasmid # 46948; http://n2t.net/addgene:46948; RRID: Addgene_46948) to generate lentivirus for transduction of B-ALL patient derived xenografts. Gene expression was confirmed by Western blotting following doxycycline exposure.
Morris V., Wang D., Li Z., Marion W., Hughes T., Sousa P., Harada T., Sui S.H., Naumenko S., Kalfon J., Sensharma P., Falchetti M., da Silva R.V., Candelli T., Schneider P., Margaritis T., Holstege F.C., Pikman Y., Harris M., Stam R.W., Orkin S.H., Koehler A.N., Shalek A.K., North T.E., Pimkin M., Daley G.Q., da Rocha E.L, & Rowe R.G. (2022). Hypoxic, glycolytic metabolism is a vulnerability of B-acute lymphoblastic leukemia-initiating cells. Cell reports, 39(4), 110752.
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2

Lentiviral Transduction of MYC in Leukemia

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human MYC cDNA was purchased from Addgene (pDONR223_MYC_WT, a gift from Jesse Boehm and Matthew Meyerson and David Root (plasmid # 82927; http://n2t.net/addgene:82927; RRID: Addgene_82927). The MYC cDNA was cloned into the pCW57.1 vector (gift from David Root (Addgene plasmid # 41393; http://n2t.net/addgene:41393; RRID:Addgene_41393) using LR clonase (Thermo). The purified plasmid was used to generate lentivirus in HEK-293T cells, which was used to transduce MV4; 11 cells. A stable transduced polyclonal line was selected with 0.5 μg/mL puromycin (Thermo). The MYC cDNA was also cloned into pINDUCER21 (ORF-EG) which was a gift from Stephen Elledge and Thomas Westbrook (Addgene plasmid # 46948; http://n2t.net/addgene:46948; RRID: Addgene_46948) to generate lentivirus for transduction of B-ALL patient derived xenografts. Gene expression was confirmed by Western blotting following doxycycline exposure.
Morris V., Wang D., Li Z., Marion W., Hughes T., Sousa P., Harada T., Sui S.H., Naumenko S., Kalfon J., Sensharma P., Falchetti M., da Silva R.V., Candelli T., Schneider P., Margaritis T., Holstege F.C., Pikman Y., Harris M., Stam R.W., Orkin S.H., Koehler A.N., Shalek A.K., North T.E., Pimkin M., Daley G.Q., da Rocha E.L, & Rowe R.G. (2022). Hypoxic, glycolytic metabolism is a vulnerability of B-acute lymphoblastic leukemia-initiating cells. Cell reports, 39(4), 110752.
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