Ten days after inoculation, the fruits were harvested. The collected fruits were separated into hull, shell, and kernel. All the samples were snap–frozen in liquid nitrogen and stored in paper bags at −80 °C for at least 24 h before being freeze–dried in an ALPHA 1–4 LD freeze dryer (Martin Christ, Osterode am Harz, Germany) at −53 °C and 0.030 m Bar pressure for 48 h. Freeze–dried samples were stored at 4 °C.
Alpha 1 4 ld freeze dryer
Manufactured by Martin Christ
Sourced in Germany
The ALPHA 1–4 LD is a laboratory freeze dryer manufactured by Martin Christ. It is designed to remove water from samples through the process of sublimation. The device can accommodate various sample sizes and configurations.
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Lab products found in correlation
5 protocols using alpha 1 4 ld freeze dryer
1
Fruit Harvesting and Freeze-Drying
2
Extraction of Bioactive Compounds from Basidiocarps
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The fresh basidiocarps were sliced and boiled with triple-distilled water at a ratio of 1:10 (w/v) with agitation at 60°C±2°C for 30 minutes.15 The boiled mushroom was left covered for 30 minutes. Residues were then removed by filtration through absorbent cotton gauze cloth and centrifuged at 10,000 rpm for 30 minutes at 4°C. Supernatants were collected and filtered through Whatman Number1 filter paper. The sample was freeze dried (Christ Alpha 1–4 LD Freeze dryer) and the hot aqueous extract (HAE) powder was stored at 4°C±2°C until further use.
3
Functional Analysis of Liposomes via FTIR
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To determine the functional groups of liposomes, lyophilized samples (ALPHA 1–4 LD freeze dryer, Martin Christ, Osterode am Harz, Germany) were formed into KBr pellets with a mass ratio of 1:100 [17 (link)]. The samples were analyzed using FTIR (4300, Shimadzu, Kyoto, Japan) from 4000 to 400 cm−1 with a minimum of 256 scans/spectrum and a constant scan speed of 4°/s.
4
Total Lipid Extraction from Microalgae
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Total lipid was extracted according to the previous method [26 (link), 27 (link)]. The C. cryptica culture were collected by centrifugation (3500 rpm, 10 min) and lyophilized overnight by an ALPHA 1–4 LD freeze dryer (Christ, Osterod, Germany). 50 mg of freeze-dried microalgal powder were put into a 100 mL centrifuge tube. 2.5 mL of chloroform, 5 mL of methanol and 2 mL of 50 mM K2HPO4 buffer (pH 7.4) were added into the centrifuge tube and shook for 2 h; After 2 h, 2.5 mL of chloroform and 2.5 mL of 50 mM K2HPO4 buffer (pH 7.4) was added into the above system again, mix uniformly and stood still. The liquid layered in the lower layer (chloroform layer) was transferred to a dry glass tube which the weight was taken as m1, and the glass tube with the liquid was put into a water bath at 60 °C until the liquid volatilizes completely, which the dried glass tube was weighed and was recorded as m2. The total lipid content (LC) was calculated as follows:
5
Tuna Lipid Extraction and Mercury Analysis
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All bigeye, yellowfin, and skipjack tuna samples (n total = 33) were collected in the western Indian Ocean during the unloading of commercial vessels (purse seine) at Victoria port (Seychelles). To test for efficiency and repeatability of different lipid extraction protocols (experiment A), we used three individuals, one per tuna species (n exp A = 3). To test for the effect of lipid extraction on Hg concentrations (experiment B), we used 10 other individuals per tuna species (n exp B = 30), all collected from the same fishing set. For these 30 individuals, fork length (FL) was measured to the lowest cm and ranged respectively for bigeye, yellowfin and skipjack from 44 to 91 cm (62 ± 17 cm), 35 to 129 cm (70 ± 35 cm), and 34 to 64 cm (45 ± 10 cm) (Supplementary Information Appendix S1). For both experiments, white muscle samples of around 20 g (wet weight) were collected in the front dorsal position, stored frozen at -20°C, freeze-dried for 48 hours with an Alpha 1-4 LD freeze-dryer (Christ, Coueron, France) and finally ground to a fine homogeneous powder using a MM400 grinder (Retsch, Eragny sur Oise, France) prior to lipid extraction and total Hg analyses.
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