The following antibodies were used on 4 μm FFPE tissue sections: SOX9 (Abcam, ab185966, 1:2000), MEIS1 (Abcam, ab19867, 1:2000), PBX1 (Novus Biologicals, NBP1–85803, 1:500), LEF1 (Abcam, ab137872, 1:100), phospho-ERK1/2 (Cell Signaling, 4370, 1:200), and phospho-Smad1/5/8 (Sigma-Aldrich, AB3848, 1:50).
Ab137872
Manufactured by Cell Signaling Technology
Ab137872 is a primary antibody produced by Cell Signaling Technology. It is designed for immunohistochemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab137872
1
Immunohistochemistry of Transcription Factors
2
Necroptosis Markers in Glioma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine the expression of three necroptosis-related genes between glioma and paracancerous tissues, 43 paired samples from Guangdong 999 Brain Hospital between 2017 and 2022 were collected for immunohistochemical (IHC) staining. The following antibodies were used: EZH2 (Abcam, ab283270), LEF1 (Abcam, ab137872), and CASP1 (Cell Signaling Technology, #98033). IHC-stained sections were imaged with a Leica microscope. For IHC quantification, the average percentage of positive cells in five random fields was calculated by Fiji.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!