After anesthesia, the standard limb lead II configuration electrocardiographic system was used for continuously monitoring cardiac conduction activity throughout the entire experiment (Powerlab/8sp system, AD Instruments, Colorado Springs, CO, USA). ECG parameters were analyzed offline with LabChart7.2.1 software (AD Instruments, Colorado Springs, CO, USA). Early cardiopulmonary resuscitation-induced arrhythmia parameters such as the incidence of cardiac arrhythmia, ventricular premature beats (VPB), ventricular tachycardia (VT), and AV block (AVB) were recorded for 5 min after ROSC and analyzed. Mice in all groups except for the sham mice underwent 5 min of cardiac arrest followed by 5 min of cardiopulmonary resuscitation. Mice in the canagliflozin group were given canagliflozin (20 mg/kg dissolved in saline) daily for 4 days. A similar volume of saline was given to mice in the control or sham group. Ag490, a STAT3 inhibitor was intravenously injected via the jugular vein at a dose of 5 mg/ kg 10 min prior to the induction of cardiac arrest. CPR, cardiopulmonary resuscitation; sham, sham-operated group; CON, control 1 3
Powerlab 8sp system
Manufactured by ADInstruments
Sourced in United States, Australia
The Powerlab/8sp system is a versatile data acquisition device designed for a wide range of physiological and biomedical applications. It features eight differential analog input channels, allowing for the simultaneous recording of multiple signals. The system is capable of sampling at high rates and provides advanced signal conditioning and processing capabilities.
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Lab products found in correlation
Labchart 7, by ADInstruments (2 mentions)
Com 2 model, by Baxter (2 mentions)
Onetouch ultra2, by LifeScan (1 mentions)
Colorimetric assay, by Cayman Chemical (1 mentions)
Albuwell m, by Exocell (1 mentions)
Grass ft03 force displacement transducer, by Natus (1 mentions)
Labchart v7, by ADInstruments (1 mentions)
10 protocols using powerlab 8sp system
1
Cardioprotective Effects of Canagliflozin
2
Pulmonary Hemodynamics in Newborn Lambs
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Lambs were cathetherized at three days old under general anesthesia (ketamine 10 mL·Kg−1–xylazine 0.04 mL·Kg−1, IM) and aseptic procedures [38 (link),39 (link)]. From day 3 onwards, we performed daily determination of the pulmonary arterial pressure (PAP) and heart rate (HR) (between 9:00 and 11:00 AM for 30 min) with an acquisition system (PowerLab/8SP System and Chart v4.1.2 Software System, ADInstruments, Sidney, Australia) connected to a computer [37 (link),39 (link)]. The cardiac output (CO) was determined in triplicate with the Swan–Ganz catheter connected to a cardiac output computer (COM-2 model, Baxter, Irvine, CA, USA). In addition, pulmonary vascular resistance (PVR) was calculated as described previously [38 (link)]. All in vivo measurements were performed on conscious and unanesthetized animals resting comfortably in a canvas sling.
3
Cardiac Hemodynamics Monitoring in Rats
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After anesthesia, a standard limb lead II configuration electrocardiographic system was attached subcutaneously to the rats by needle electrodes. ST-segment (the period between the end of the QRS complex and the beginning of the T wave) duration was monitored throughout the experiment using a Powerlab/8sp system (AD Instruments, Colorado Springs, CO, USA). Changes inST segment duration were quantified offline usingLabChart7.2.1 software (AD Instruments, Colorado Springs, CO, USA).
Hemodynamics were recorded from the time period of Baseline 1 to Baseline 2 and before or after myocardial ischemia. The fur in neck regions of the rats was then removed and disinfected with ethanol (70%). The skin were cut and the subcutaneous tissues were freed to expose the right carotid artery next to the trachea. A 20-G heparin-filled catheter (Spacelabs Medical, Inc., Redmond, WA, USA) was introduced into the right carotid artery and advanced into the left ventricle for measurement. Hemodynamic parameters including left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), and maximum rate of increase/decrease in left ventricular pressure (±dP/dtmax) were recorded by the connected calibrated pressure transducer with physiologic recorder (Taimeng, Chengdu, China). Data were analyzed offline with Biolap 420F software (Taimeng, Chengdu, China).
Hemodynamics were recorded from the time period of Baseline 1 to Baseline 2 and before or after myocardial ischemia. The fur in neck regions of the rats was then removed and disinfected with ethanol (70%). The skin were cut and the subcutaneous tissues were freed to expose the right carotid artery next to the trachea. A 20-G heparin-filled catheter (Spacelabs Medical, Inc., Redmond, WA, USA) was introduced into the right carotid artery and advanced into the left ventricle for measurement. Hemodynamic parameters including left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), and maximum rate of increase/decrease in left ventricular pressure (±dP/dtmax) were recorded by the connected calibrated pressure transducer with physiologic recorder (Taimeng, Chengdu, China). Data were analyzed offline with Biolap 420F software (Taimeng, Chengdu, China).
4
Cardiac Conduction Monitoring Protocol
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A standard limb lead II configuration electrocardiographic system was used for monitoring cardiac conduction activity throughout the experiments (Powerlab/8sp system, AD Instruments, Colorado Springs, CO, USA). After anesthesia, the needle electrodes were placed under the skin. Heart rate, RR, PR, QRS, and QT intervals were measured, and corrected QT interval (QTc) was calculated based on Mitchell’s formular [13 (link)]: QTc = QT/(RR/100)1/2.
5
Arteriovenous Fistula Blood Flow Measurement
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Blood blow was measured in the carotid artery before and after fistula creation, and in the jugular vein after fistula creation, using a flow probe (1 or 2 RB, Transonic Systems) connected to a transit time perivascular flowmeter (T420, Transonic Systems) [27 (link)]. Blood flow rate was recorded through a data acquisition system (PowerLab/8sp System, ADInstruments, Castle Hill, NSW, Australia). Shear stress was estimated using the Poiseuille formula τ = 4ηQ/(πRi3), where τ is wall shear stress, η is blood viscosity, Q is blood flow rate, and Ri is the internal radius. It has been demonstrated that Poiseuille's law can be applied to the flow within blood vessels of diameters greater than 0.1 mm [32 ]. Therefore, it is applicable to this arteriovenous fistula model.
6
Cardiovascular Arrhythmia Assessment
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ECG parameters were recorded throughout the experiments using a PowerLab/8sp system (AD Instruments, Colorado Springs, CO). A standard limb lead 2 configuration system was used and needle electrodes were attached to the mice underneath the skin. LabChart 7.2.1 software (AD Instruments) was used to analyze the following arrhythmia events recorded during the 20 min of reperfusion injury period: (1) Number of mice with any arrhythmia ‐ AV block (AVB); sudden cardiac death (SCD); ventricular tachycardia (VT); polymorphic VT (PVT); or sustained VT (>10 sec) (SVT). (2) Duration of VT. (3) Start time of the first run of ventricular tachycardia.
7
Comprehensive Metabolic and Functional Evaluation
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Random blood glucose was measured by glucose oxidase biosensor (OneTouch Ultra-2; LifeScan), and BP was measured under anesthesia and analyzed using PowerLab/8SP system (Chart; AD Instruments, Colorado Springs, CO, Unites States) as previously described (Veron et al., 2011 (link); Veron et al., 2012 (link)). Plasma and urine creatinine were measured by HPLC, and glomerular filtration rate (GFR) was assessed by creatinine clearance. Albuminuria was evaluated by Coomassie blue staining and measured by ELISA (Albuwell-M, Exocell), plasma and urine VEGF-A were quantified by ELISA (R&D), NO was measured by colorimetric assay (Cayman), as previously described (Veron et al., 2014 (link)), and urine thiols (Cys and GSH) were measured by fluorometric assay (Cayman), following manufacturers’ protocol.
8
Langendorff-Perfused Mouse Heart Assay
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Mice were anaesthetised by intra-peritoneal injection of pentobarbitone 140 mg/kg. Beating hearts were rapidly excised, cannulated and perfused in the isovolumic Langendorff mode at 80 mmHg perfusion pressure, 37°C with Krebs-Henseleit (KH) buffer continuously gassed with 95% O2/5% CO2 (pH 7.4) containing (in mM): NaCl 120, KCl 4.7, MgSO4.7H2O 1.2, NaHCO3 25, KH2PO4 1.2, CaCl2.H2O 1.8, glucose 11. Cardiac function was assessed using a fluid-filled cling film balloon inserted via the mitral valve into the left ventricle (LV), and connected via a line to a pressure transducer (Memscap) and a Powerlab/8 SP system (AD Instruments). The intraventricular volume was adjusted to achieve an initial LV diastolic pressure of 10–15 mmHg [23] (link). Functional parameters were averaged for ∼80 cardiac cycles at 5 min perfusion intervals. Left ventricular developed pressure (LVDP) was calculated as the difference between systolic (SP) and diastolic pressures (DP) [25] (link). Rate pressure product (RPP) was determined as the product of heart rate and LVDP. At the end of each perfusion, hearts were freeze clamped using Wollenberger tongs, cooled in liquid nitrogen and stored at –80°C until further analysis.
9
Isolated Perfused Mouse Heart Protocol
10
Cardiovascular Hemodynamics Measurement
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Pulmonary arterial pressure (PAP), systemic arterial pressure (SAP), and heart rate (HR) were recorded via a data acquisition system (PowerLab/8SP System and LabChart v7.0 Software; ADInstruments) connected to a computer. Cardiac output (CO) was determined with the thermodilution method by the injection of 3 ml of chilled (0°C) 0.9% NaCl into the pulmonary artery through the Swan-Ganz catheter connected to a CO computer (COM-2 model, Baxter). Mean PAP (mPAP), mean SAP (mSAP), and pulmonary (PVR) vascular resistances were calculated as described previously, Herrera et al. (2007 (link)).
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