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Common reagents

Manufactured by Merck Group
Sourced in Spain, United States

Common reagents are laboratory chemicals and substances used in various scientific experiments and procedures. These reagents serve as essential components in chemical reactions, analysis, and other laboratory activities. They assist in processes such as identifying, isolating, and quantifying specific substances or compounds. The core function of common reagents is to facilitate and support a wide range of scientific investigations and laboratory work.

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3 protocols using common reagents

1

Reagent Procurement for Cell Assays

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Common reagents were purchased from Sigma-Aldrich (St. Louis, MO), unless otherwise noted. Cell culture medium and reagents were purchased from Corning (Corning, NY). Fetal bovine serum was purchased from Atlanta Biologicals (Lawrenceville, GA). Recombinant human GH was kindly provided by Eli Lilly (Indianapolis, IN).
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2

Cytokine Assay Protocol Optimization

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Common reagents were from Sigma-Aldrich-Merck (Madrid, Spain) or Roche (Darmstadt, Germany). Human cytokines were from PeproTech (London, UK) or Merck. Tissue culture dishes were from Falcon (Lincoln Park, NJ, USA), and serum and culture media were from Invitrogen (Life Technologies/Thermo-Fisher, Madrid, Spain).
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3

Radiolabeling EGFR-Targeting Antibody C225

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Common reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Acros (Geel, Belgium) and were used as received unless otherwise specified. Methoxy-sulfohydro-PEG (SH-PEG, molecular weight 5000) was obtained from Sigma-Aldrich. The monoclonal anti-EGFR antibody C225 was obtained from ImClone Systems (New York, NY, USA). C225 is a chimeric human–mouse immunoglobulin G1 that binds EGFR with high affinity.23 (link),24 (link) Recombinant human EGFR/ErbB1 Fc chimera (rhEGF) was obtained from R&D Systems (Minneapolis, MN, USA). Tris(2-carboxyethyl)phosphine was purchased from Pierce Biotechnology (Rockford, IL, USA). NAP-5 columns were obtained from GE Healthcare (Illustra, Buckinghamshire, UK). Indium-111 chloride (111InCl3) was obtained from Perkin–Elmer (Waltham, MA, USA). Isoflurane was obtained from Henry Schein Animal Health (formerly Butler Animal Health Supply, Dublin, OH, USA). All the chemicals and solvents were at least American Chemical Society grade and were used without further purification.
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