Log In Sign up
The largest database of trusted experimental protocols

N methyl n tert butyldimethylsilyl trifluoroacetamide mtbstfa

Manufactured by Merck Group
Visit Supplier
Sourced in United States

N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) is a silylating agent used in analytical chemistry. It is a colorless liquid that reacts with alcohols, phenols, and other compounds containing active hydrogen atoms to form trimethylsilyl (TMS) derivatives.

Automatically generated - may contain errors

Lab products found in correlation

Toluene, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Water purification system, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by GE Healthcare (1 mentions) Ammonium acetate nh4oac, by Thermo Fisher Scientific (1 mentions) Oasis hlb cartridge, by Waters Corporation (1 mentions) Acetic anhydride, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Ethyl acetate, by Merck Group (1 mentions) Dichloromethane, by Thermo Fisher Scientific (1 mentions) Ethyl acetate, by Thermo Fisher Scientific (1 mentions)

5 protocols using n methyl n tert butyldimethylsilyl trifluoroacetamide mtbstfa

1

Metabolomics Sample Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetonitrile (ACN), methanol (MeOH), and ammonium acetate (NH4Oac), all LC–MS grade, were purchased from Fisher Scientific (Pittsburgh, PA). Ammonium hydroxide (NH4OH), O-methylhydroxylamine hydrochloride (MeOX), and N-Methyl-n-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) were bought from Sigma-Aldrich (Saint Louis, MO). Deionized water was provided in-house by a water purification system from EMD Millipore (Billerica, MA). Phosphate buffered saline (PBS) was bought from GE Healthcare Life Sciences (Logan, UT). Standard compounds corresponding to measured aqueous metabolites/features were purchased from Sigma-Aldrich and Fisher Scientific. Lipid standards were purchased from Fisher Scientific, Sigma-Aldrich, and Avanti Polar Lipids (Alabaster, AL).
Jasbi P., Mohr A.E., Shi X., Mahmood T., Zhu Q., Bruening M., Gu H, & Whisner C. (2022). Microbiome and metabolome profiles of high screen time in a cohort of healthy college students. Scientific Reports, 12, 3452.
+ Open protocol
+ Expand
2

Triclosan Quantification in Environmental Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Methanol (MeOH) (HPLC grade), acetonitrile, ethyl acetate, and formic acid were purchased from Merck (Darmstadt, Germany). Triclosan (TCS) and triclosan 13C6 (TCS-13C6) were provided by Toronto Research Chemicals Inc. (North York, ON, Canada). Acetic anhydride and the silylation agent N-methyl-N-(tert-butyldimethylsilyl)-trifluoroacetamide (MTBSTFA) were provided by Sigma-Aldrich (Milwaukee, WI, USA). Individual stock standards of TCS and TCS-13C6 were prepared in MeOH. Further dilutions were made in the same solvent, and stored at − 20 °C. Calibration standards for DART were prepared in ethyl acetate from methanol stocks.
OASIS HLB cartridges (60 mg) were obtained from Waters (Milford, MA, USA), C18 was provided by Agilent (Wilmington, DE, USA), diatomaceous earth was provided by VWR Chemicals (Leicestershire, England), and 10-mL polypropylene syringes were purchased from Becton Dickinson (Franklin Lakes, NJ, USA).
Cobo-Golpe M., García-Martín J., Ramil M., Cela R, & Rodríguez I. (2021). Assessment of direct analysis in real time accurate mass spectrometry for the determination of triclosan in complex matrices. Analytical and Bioanalytical Chemistry, 413(25), 6355-6364.
+ Open protocol
+ Expand
3

Characterization of PAHs and Standards

Check if the same lab product or an alternative is used in the 5 most similar protocols
Information for PAHs and deuterated or isotopically labelled standards, including abbreviations, is provided in the Supporting Information (Table A1). Solvents including: acetonitrile (ACN), acetone (Ace), ethyl acetate (EA), dichloromethane (DCM), hexane, and methanol (MetOH); all optima grade were purchased from Thermo Fisher Scientific (Santa Clara, CA). Toluene, dimethylsulfoxide (DMSO), N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA), dithiobis-2-nitrobenzoic acid (DTNB), dithiothreitol (DTT), and 1,4-naphtoquinone were purchased from Sigma-Aldrich (Milwaukee, WI).
Roper C., Perez A., Barrett D., Hystad P., Massey Simonich S.L, & Tanguay R.L. (2020). Workflow for Comparison of Chemical and Biological Metrics of Filter Collected PM2.5. Atmospheric environment (Oxford, England : 1994), 226, 117379.
+ Open protocol
+ Expand
4

Native PAHs Analytical Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Native stock solutions of unsubstituted PAHs (n = 21), OHPAHs (n = 38), OPAHs (n = 24), HMW-PAHs (n = 14), NPAHs (n = 23), and isotopically labeled standards were purchased from different vendors. All 120 PAHs studied and isotopically labeled standards, their abbreviations, and vendors are listed in the Supporting Information (Table S1 and S2). The solid-phase extraction (SPE) Bond Elut Si (20 g, 60 mL) cartridges were purchased from Agilent Technologies (New Castle, DE) and the ISOLUTE ENV+ cartridges (100 mg, 6mL) were purchased from Biotage (Charlotte, NC). All solvents (methanol (MetOH), hexane (Hex), ethyl acetate (EA), acetonitrile (ACN), acetone (Ace), and dichloromethane (DCM); all optima grade) and 20 mL clear glass vials were purchased from Thermo Fisher Scientific (Santa Clara, CA). Toluene (Tol) (≥ 99.9%) and the derivatizing agent N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) (>97%) were purchased from Sigma-Aldrich (Milwaukee, WI).
Trine L.S., Davis E.L., Roper C., Truong L., Tanguay R.L, & Massey Simonich S.L. (2019). Formation of PAH derivatives and increased developmental toxicity during steam enhanced extraction remediation of creosote contaminated Superfund soil. Environmental science & technology, 53(8), 4460-4469.
+ Open protocol
+ Expand
5

PAHs and Isotopic Standards Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
PAHs and isotopically labeled standards information, including abbreviations and vendors, is provided in the Supporting Information (Table S1). Solvents including: methanol (MetOH), hexane, ethyl acetate (EA), acetonitrile (ACN), acetone (Ace), and dichloromethane (DCM); all optima grade were purchased from Thermo Fisher Scientific (Santa Clara, CA). Toluene, dimethylsulfoxide (DMSO), and N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) were purchased from Sigma-Aldrich (Milwaukee, WI).
Roper C., Delgado L.S., Barrett D., Simonich S.L, & Tanguay R.L. (2018). PM2.5 Filter Extraction Methods: Implications for Chemical and Toxicological Analyses. Environmental science & technology, 53(1), 434-442.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!