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δ tocotrienol

Manufactured by Cayman Chemical

δ-Tocotrienol is a laboratory product available from Cayman Chemical. It is a naturally occurring form of vitamin E. The core function of δ-Tocotrienol is to serve as a research tool for scientific investigations.

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2 protocols using δ tocotrienol

1

Tocotrienol Purity Characterization

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δ-Tocotrienol, γ-tocotrienol, and α-tocotrienol were purchased from Cayman Chemical, i-DNA (M) Sdn. Bhd, Malaysia (Cayman Chemical, USA). The purity of each T3 was ≥98%.
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2

Modulation of THP-1 and DOK cell responses by ANE and arecoline

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THP-1 cells were seeded in 6 cm dishes at a density of 2 × 10 6 , primed with 50 nM PMA for 6 h and refreshed with 5 mL culture medium. The cells were then treated with ANE (0.4 mg/mL for THP-1 cells and DOK cells; 0.1 µg/ml for PBMCs) or arecoline (0.1 mM and 0.3 mM) for 48 h. For functional studies, the CREB inhibitor 666-15 (80 nM, TOCRIS), the CREB inhibitor KG-501 (10 μM, MedchemExpress) [25, 26] which is a gift from lab of Prof. Long-Sen Chang [27] (link), the VCAM-1 inhibitor δ-tocotrienol (20 µM, Cayman) was added to the culture media 30 min before adding ANE. Conditioned media were collected at 48 h after ANE treatment.
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