Mouse nt probnp elisa kit

Frozen human plasma samples were used to measure LIGHT levels. Mouse serum samples or BMDM supernatants were used to measure the levels of TGF-β1, IL-10, IL-4, TNF-α, and IL-1β. ELISA was performed using commercial kits [the Human LIGHT/LIGHT Quantikine ELISA Kit (R&D Systems Inc., Minneapolis, USA, cat#DLIT00), Mouse Transforming Growth Factor Beta 1 ELISA Kit (ABclonal, Wuhan, China, cat#RK0057), Mouse IL-10 ELISA Kit (ABclonal, cat#RK00016), Mouse IL-4 ELISA Kit (ABclonal, cat#RK00036), Mouse TNF-α ELISA Kit (Elabscience Biotechnology Co., Ltd, Wuhan, China, cat#E-EL-M3063), Mouse NT-proBNP ELISA Kit (Elabscience Biotechnology Co., Ltd, cat# E-EL-M0834c) and IL-1 beta Mouse ELISA Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA, cat#88-7013-77)] according to the manufacturer’s instructions.

ELISA analysis was performed according to the manufacturer’s instructions. The plasmic NE level in each group of mice was measured using an NE ELISA kit (NOU39-K010, Eagle Biosciences). The plasmic NT-proBNP level in mice was measured using a Mouse NT-proBNP ELISA Kit (E-EL-M0834c, Elabscience). The pro-inflammatory cytokines’ level in the cellular supernatant was measured by TNF-α ELISA Kit (ab208348, Abcam) and IL-1β Mouse ELISA Kit (BMS6002, Thermo).

Cardiac tissue powder, cardiac primary cells and HEK 293T cells were lysed in RIPA buffer containing proteases/phosphatase inhibitors (Roche, Mannheim, Germany), sonicated and centrifuged. Proteins (15–25 µg) were loaded on SDS-PAGE and electro-transferred onto nitrocellulose membranes. Membranes were incubated with primary and secondary antibodies (listed in Supplementary Table 2) and revealed with ECL Reagent (GE Healthcare Life Sciences, Chalfont, United Kingdom). Cardiac tissue levels of IL-6 and MCP-1 were measured using mouse IL-6 DuoSet (DY-406) and mouse CCL2/JE/MCP-1 DuoSet (DY479) ELISA kits (R&D Sytems, Minneapolis, USA). Plasma NT-proBNP was measured with the Mouse NT-proBNP ELISA kit from Elabscience (Houston, USA).