For nicotine dose estimates on the DissolvIt glass coverslips and for the particle size distribution analyses, a standard HPLC instrument (PerkinElmer Altus A-10; Waltham, MA, USA) was used with a reversed-phase C18 column (Agilent Eclipse XDB-C18, 5 mm, 4.6 Â 25 mm; Santa Clara, CA, USA) and a photodiode array detector (PerkinElmer Altus A10). The mobile phase used was 99% ammonium acetate buffer (pH 4.0) with 1% acetonitrile (channel A) and 100% acetonitrile (channel B). The flow was isocratic, with the composition 92.5% A and 7.5% B. The detection wavelength was set to 260 nm. For each injection, 100 mL were used with a run time of six minutes. Samples were diluted in 8.5% acetonitrile mixed with 91.5% mobile phase A (named 'diluent'). The standard curve dilutions were prepared in triplicate by diluting nicotine bitartrate dihydrate USP reference standard (USP, Rockville, MD, USA) in the diluent to five concentrations between 41 ng/mL and 501 ng/mL, yielding 15 concentrations in total. With the Log-Log Linear fit type, the curve had the correlation factor r 2 ¼0.997. HPLC was controlled using the Waters Empower 3 software (Waters Corporation, Milford, MA, USA).
Altus a 10
Manufactured by PerkinElmer
Sourced in United States
The Altus A-10 is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a robust and reliable design, delivering accurate and reproducible results across a wide range of chromatographic methods.
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Lab products found in correlation
3 protocols using altus a 10
1
Nicotine Quantification on DissolvIt Coverslips
2
Sludge Characterization and Cracking Degree
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The sludge samples
were centrifuged at 9000 rpm for 10 min, and the supernatant was decanted
and filtered through a 0.45 μm filter membrane for analysis.
COD was determined by fast digestion spectrophotometry; TOC was determined
by a total organic carbon analyzer; carbohydrate used anthracene method
with glucose as the standard to determine absorbance at 625 nm; protein
used the Coomassie Brilliant G250 method with bovine serum protein
as the standard to determine the absorbance of the sample at 595 nm.41 (link) DNA was determined by the diphenylamine method.
VFAs were determined by high-performance liquid chromatography (PerkinElmer,
Altus A-10). The sample was centrifuged at 9000 rpm for 15 min and
passed through a 0.25 μm filter membrane. The column model was
AminexHPX-87H (BioRad), and the column temperature was 35 °C.
To further clarify the degree of sludge cracking, the sludge cracking
degree was used to characterize the degree of sludge cracking, which
can be calculated as follows42 (link) where ρ(SCODafter) is the
treated dissolved COD concentration, ρ(SCOD0) is
the untreated dissolved COD concentration, and ρ(TCOD0) is the total COD concentration in the excess sludge.
were centrifuged at 9000 rpm for 10 min, and the supernatant was decanted
and filtered through a 0.45 μm filter membrane for analysis.
COD was determined by fast digestion spectrophotometry; TOC was determined
by a total organic carbon analyzer; carbohydrate used anthracene method
with glucose as the standard to determine absorbance at 625 nm; protein
used the Coomassie Brilliant G250 method with bovine serum protein
as the standard to determine the absorbance of the sample at 595 nm.41 (link) DNA was determined by the diphenylamine method.
VFAs were determined by high-performance liquid chromatography (PerkinElmer,
Altus A-10). The sample was centrifuged at 9000 rpm for 15 min and
passed through a 0.25 μm filter membrane. The column model was
AminexHPX-87H (BioRad), and the column temperature was 35 °C.
To further clarify the degree of sludge cracking, the sludge cracking
degree was used to characterize the degree of sludge cracking, which
can be calculated as follows42 (link) where ρ(SCODafter) is the
treated dissolved COD concentration, ρ(SCOD0) is
the untreated dissolved COD concentration, and ρ(TCOD0) is the total COD concentration in the excess sludge.
3
Sugar Content Analysis of Fruit Cultivars
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The analysis and extraction of sugar content was modified from a method put forward by previous reports [22 ]. Briefly, juice sacs of three cultivars were frozen in liquid nitrogen and ground into powder. A total of 3 g of powdered sample was weighed and extracted with 3 mL deionized water by vortexing for 2 min and followed by ultrasonic extraction at 40 °C for 1 h. The extract was centrifuged at 12,000× g for 10 min and the supernatant was subjected to HPLC analysis. Extracts were analyzed by ALTUS A-10 (Perkin Elmer) consisting of a differential refraction detector, equipped with Durashell amino column (5 μm, 4.6 × 250 mm). The column temperature was 40 °C. Injection volume was 20 μL, 70% acetonitrile and 30% deionized water were used as mobile phases at a flow rate of 1 mL min−1. The sugar concentration was obtained from the sugar standard curve. Sugar contents were expressed as mg g−1. The contents of sugars were calculated using the following equation:
where C is the sugar concentration obtained from the sugar standard curve, C0 is the sugar concentration of blank control, and n is the dilution ratio. V is the volume and m is the weight of samples. Total soluble sugar was determined according to previously reported methods [23 (link)].
where C is the sugar concentration obtained from the sugar standard curve, C0 is the sugar concentration of blank control, and n is the dilution ratio. V is the volume and m is the weight of samples. Total soluble sugar was determined according to previously reported methods [23 (link)].
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