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SF3A2 is a small nuclear ribonucleoprotein complex subunit that plays a role in pre-mRNA splicing. It is involved in the recognition of the 3' splice site during the earliest steps of spliceosome assembly.

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Lab products found in correlation

Sr140, by Santa Cruz Biotechnology (2 mentions) Sf3b1, by Santa Cruz Biotechnology (2 mentions) Goat anti mouse alexa fluor 555 antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Snrpa, by Santa Cruz Biotechnology (1 mentions) Tfiiic110, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Strap, by BD (1 mentions)

2 protocols using sf3a2

1

Immunofluorescence Staining of Splicing Factors

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Cells were fixed in 4% paraformaldehyde for 10 min at room temperature, blocked, and then incubated at 4 °C overnight with the following antibodies: SR140 (at the dilution of 1:150, Santa Cruz, sc-398718); SF3A2 (at the dilution of 1:150, Santa Cruz, sc-390444); SF3B1 (at the dilution of 1:150, Santa Cruz, sc-514655); SYF1 (at the dilution of 1:150, Santa Cruz, sc-271037); or STRAP (at the dilution of 1:200, Bethyl, A304-735A). After washing, cells were incubated with goat anti-rabbit Alexa Fluor 488 antibody (at the dilution of 1:150, Life Technologies, A-11008) or goat anti-mouse Alexa Fluor 555 antibody (at the dilution of 1:150, Life Technologies, A-21422) and counter-stained with DAPI to detect nuclei.
Jin L., Chen Y., Crossman D.K., Datta A., Vu T., Mobley J.A., Basu M.K., Scarduzio M., Wang H., Chang C, & Datta P.K. (2020). STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells. Nature Communications, 11, 5941.
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2

Western Blot Profiling of Spliceosomal Proteins

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Proteins were separated by 10% SDS-PAGE and probed with primary antibodies. Primary antibodies, at the dilution of 1:1000 for each, included: SNRPA (Santa Cruz, sc-376027); U2A’ (Santa Cruz, sc-393804); SR140 (Santa Cruz, sc-398718); HELIC2 (Santa Cruz, sc-393170); PRPF3 (Santa Cruz, sc-101130); SYF1 (Santa Cruz, sc-271037); SF3A3 (Santa Cruz, sc-393673); SF3A2 (Santa Cruz, sc-390444); SF3B1 (Santa Cruz, sc-514655); STRAP (BD Transduction Labs, #611346); DDX15 (Santa Cruz, sc-271686); TFIIIC110 (Santa Cruz, sc-81406); GAPDH (Cell Signaling, #2118), and CHERP (Santa Cruz, sc-100650). β-Actin (Sigma, A5316) was used at the dilution of 1:10,000.
Jin L., Chen Y., Crossman D.K., Datta A., Vu T., Mobley J.A., Basu M.K., Scarduzio M., Wang H., Chang C, & Datta P.K. (2020). STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells. Nature Communications, 11, 5941.
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