Anti pink1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-PINK1 is a laboratory reagent used for the detection and quantification of PINK1 (PTEN-induced putative kinase 1) protein. PINK1 is a serine/threonine-protein kinase that plays a role in mitochondrial homeostasis and is associated with Parkinson's disease. The Anti-PINK1 product provides a tool for researchers to study the expression and function of PINK1 in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

PINK1 (20 citations) Rabbit anti-PINK1 (3 citations)

6 protocols using anti pink1

Western Blot Analysis of Autophagy Markers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analysis was conducted as previously described44 (link). The following primary (at a concentration of 1.5 μg ml⁻¹) and secondary (0.5 ng ml⁻¹) antibodies were used for western blotting: polyclonal anti-ARRDC1, anti-LC3, anti-MDA5, anti-TLR9 (Abcam), polyclonal anti-Atg-12 (Santa Cruz Biotechnology, SC-68884), polyclonal anti-Pink1 (Santa Cruz Biotechnology, SC-33796), anti-Parkin mouse monoclonal (clone PRK8, Santa Cruz Biotechnology), polyclonal anti-Miro (Santa Cruz Biotechnology, SC-292547), anti-CD9 (MM2/57, Millipore), anti-CD63 (MEM-259, ThermoFisher), anti-TSG101 (51/TSG101, BD Biosciences), anti-Human MFGE8 (Abnova), anti-Dicer (D11, Santa Cruz Biotechnology, used at a concentration of 2 μg ml⁻¹), anti-Tubulin, anti-LC3 (rabbit polyclonal), anti-TLR-7 (Cell Signaling Technology), Beta actin (AC-15, Sigma-Aldrich) and anti-GAPDH (Santa Cruz Biotechnology, SC-25778). NF-κB nuclear translocation was characterized by staining cells with anti NF-κB-P50 Alexa Fluor 488 (E-10, Santa Cruz Biotechnology). Images were obtained using a Leica TCS NT upright confocal microscope.

Phinney D.G., Di Giuseppe M., Njah J., Sala E., Shiva S., St Croix C.M., Stolz D.B., Watkins S.C., Di Y.P., Leikauf G.D., Kolls J., Riches D.W., Deiuliis G., Kaminski N., Boregowda S.V., McKenna D.H, & Ortiz L.A. (2015). Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nature Communications, 6, 8472.

+ Open protocol

+ Expand

Protein Expression Analysis in Mouse Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse tissue samples or cells were homogenized in lysis buffer containing 1% protease inhibitors. Protein assay kit was used to determine the protein concentration. The antibodies used in this study were anti-HSF2(Santa Cruz, 1:1000), anti-PARL (1:1000, Santa Cruz), anti-PINK1 (1:1000, Santa Cruz), anti-Parkin (1:1000, Santa Cruz), anti-β-actin (1:5000, Abcam), and anti-GAPDH (1:5000, Abcam). The results were analyzed by ImageJ.

Liang H., Zhang F., Wang W., Zhao W., Zhou J., Feng Y., Wu J., Li M., Bai X., Zeng Z., Niu J, & Miao Y. (2022). Heat Shock Transcription Factor 2 Promotes Mitophagy of Intestinal Epithelial Cells Through PARL/PINK1/Parkin Pathway in Ulcerative Colitis. Frontiers in Pharmacology, 13, 893426.

+ Open protocol

+ Expand

Western Blot Analysis of Autophagy and Inflammation Markers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analysis was conducted as previously described ^{44 (link)}. The following primary (at a concentration of 1.5 μg/ml) and secondary (0.5 ng/ml) antibodies were used for Western Blotting: polyclonal anti-ARRDC1, anti-LC3, anti-MDA5, anti-TLR9 (Abcam), polyclonal anti-Atg-12 (Santa Cruz Biotechnology, SC-68884), polyclonal anti-Pink1 (Santa Cruz Biotechnology, SC-33796), anti-Parkin mouse monoclonal (clone PRK8, Santa Cruz Biotechnology), polyclonal anti-Miro (Santa Cruz Biotechnology, SC-292547), anti-CD9 (MM2/57, Millipore), anti-CD63 (MEM-259, ThermoFisher), anti-TSG101 (51/TSG101, BD Bioscciences), anti-Human MFGE8 (Abnova), anti-Dicer (D11, Santa Cruz Biotechnology, used at a concentration of 2 μg/ml), anti-Tubulin, anti-LC3 (rabbit polyclonal), anti-TLR-7 (Cell Signaling Technology), Beta actin (AC-15, Sigma Aldrich), anti-GAPDH (Santa Cruz Biotechnology, SC-25778). NFκB nuclear translocation was characterized by staining cells with anti NFκB -P50 Alexa Fluor 488 (E-10, Santa Cruz). Images were obtained using a Leica TCS NT upright confocal microscope.

+ Open protocol

+ Expand

Mitochondrial Dynamics Regulate TRAIL-Induced Apoptosis in NSCLC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human NSCLC cell lines (Calu-1, A549, and H460) and BEAS-2B normal bronchial epithelial cells were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China) and grown in RPMI-1640 medium (Gibco, Grand Island, NY, USA), supplemented with 10% heat-inactivated fetal bovine serum and 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) in a humidified atmosphere of 95% air and 5% CO₂ at 37°C. Recombinant human TRAIL was purchased from PeproTech (Rocky Hill, NJ, USA). Mouse monoclonal anti-Drp1, anti-phosphorylated (p)-Drp1(Ser616), anti-caspase-3, anti-PARP, anti-LC3, anti-p62, anti-COX IV, anti-PINK1, and anti-Parkin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse monoclonal anti-Fis1, anti-Mfn1, anti-Mfn2, anti-Opa1, anti-β-actin, N-acetyl-l-cysteine (NAC), and mitochondrial division inhibitor 1 (Mdivi-1) were purchased from Sigma (St Louis, MO, USA). Mn(III) tetrakis (1-methyl-4-pyridyl) porphyrinpentachloride (MnTMPyP) was obtained from Cayman Chemical (Ann Arbor, MI, USA).

Ke S., Zhou T., Yang P., Wang Y., Zhang P., Chen K., Ren L, & Ye S. (2017). Gold nanoparticles enhance TRAIL sensitivity through Drp1-mediated apoptotic and autophagic mitochondrial fission in NSCLC cells. International Journal of Nanomedicine, 12, 2531-2551.

+ Open protocol

+ Expand

Protein Extraction and Quantification

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were extracted, quantified and analyzed as reported previously (Xiong et al. 2018 (link); Liu et al. 2019 (link); An et al. 2021 (link)). The primary and secondary antibodies used included anti-β-MHC, anti-ANP, anti-HO1, anti-GPX4 (1:1000, Proteintech, USA), anti-PINK1 (1:200, Santa Cruz, USA), anti-SIRT1 (1:1000, Cell Signaling Technology, USA), goat anti-rabbit and anti-mouse IgG-HRP (1:5000, Fudebio, China) antibodies.

Yu H., Gan D., Luo Z., Yang Q., An D., Zhang H., Hu Y., Ma Z., Zeng Q., Xu D, & Ren H. (2024). α-Ketoglutarate improves cardiac insufficiency through NAD+-SIRT1 signaling-mediated mitophagy and ferroptosis in pressure overload-induced mice. Molecular Medicine, 30, 15.

+ Open protocol

+ Expand

Mitochondrial Dysfunction in Parkinson's Disease

Check if the same lab product or an alternative is used in the 5 most similar protocols

Materials used include: MPP⁺Iodide (Abcam), dimethyl fumarate (DMF) (Sigma Aldrich), JC-1 stain (Invitrogen, 3168), MTT (Invitrogen, M6494), DMSO (Sigma-Aldrich, D8418), Chemiluminescence western blotting substrate (Bio-Rad Laboratories, 1705061), Sucrose (Hi-media, MB025), OCT media (Sigma-Aldrich, SHH0026), T-PER extraction buffer (ThermoFisher, 78510), protease inhibitor (Sigma-Aldrich, P8340), Nitrocellulose membrane (GC-NCM-302), Bovine serum albumin (SRL, A6003), anti-Tyrosine Hydroxylase (TH) (Santa Cruz Biotechnology, sc25269), anti-α-synuclein (Santa Cruz Biotechnology, sc53955), anti-β-actin (Santa Cruz Biotechnology, sc47778), anti-NRF1 (Cell Signaling Technology, 12381S), anti-NRF2 (Sigma-Aldrich, SAB5700720), anti-PINK1 (Santa Cruz Biotechnology, sc517353), anti-Parkin (Santa Cruz Biotechnology, sc30130), LC-3A/B (Cell Signaling Technology, 12741S), anti-mouse Alexa flour 488 (A21202LC3), BECLIN-1 (3495S), BCL2 (NBP2-67182), anti-mouse secondary antibody (HRP-conjugated) (7076s), anti-rabbit secondary (HRP-conjugated) (AP307P), fluoroshield DAPI (F6057), Alexa fluor 488 Donkey Anti-Mouse IgG (H + L) (A-11008). All the chemicals used in the study were obtained from commercial vendors unless otherwise specified.

Pinjala P., Tryphena K.P., Kulkarni A., Goswami P.G, & Khatri D.K. (2024). Dimethyl Fumarate Exerts a Neuroprotective Effect by Enhancing Mitophagy via the NRF2/BNIP3/PINK1 Axis in the MPP+ Iodide-Induced Parkinson’s Disease Mice Model. Journal of Alzheimer's Disease Reports, 8(1), 329-344.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!