Antibodies to flag

Manufactured by Merck Group

Antibodies to FLAG are a type of laboratory reagent used to detect and purify proteins that have been engineered to contain a specific peptide tag called the FLAG tag. The FLAG tag is a short amino acid sequence that can be attached to a protein of interest, allowing it to be recognized and bound by the anti-FLAG antibodies. These antibodies can be used in various techniques, such as immunoprecipitation, Western blotting, and affinity chromatography, to isolate and study the tagged proteins.

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Mouse monoclonal antibodies to FLAG (2 citations)

2 protocols using antibodies to flag

Antibody-based Assays for DNA Damage

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Antibodies to FLAG (catalog no. F1804) and ATM (catalog no. PLA0086) were purchased from Sigma. Antibodies to phospho-histone-H2AX (Ser¹³⁹) (catalog no. 9718), and DYKDDDDK (equivalent to FLAG, catalog no. 8146) were purchased from Cell Signaling Technology. Antibodies to RNF20 (catalog no. ab181104), SMURF2 (catalog no. ab53316), and GST (catalog no. ab19256) were obtained from Abcam. Anti-HSC70 (catalog no. sc-7298) was purchased from Santa Cruz. Cycloheximide was purchased from Merck Bioscience. Etoposide and KU60019 (ATM inhibitor) were purchased from Sigma. Purified GST–ATM protein (catalog no. A26-35G) for the in vitro binding assay was obtained from SignalChem, and purified active ATM for in vitro kinase assay was purchased from Sigma (catalog no. 14-933M). Agarose-conjugated anti-FLAG (catalog no. A2220), FLAG peptide (catalog no. F3290), and anti–HA-peroxidase (catalog no. 12013819001) were obtained from Sigma. All other antibodies and reagents used in this study have been described previously (14 (link)).

Tang L.Y., Thomas A., Zhou M, & Zhang Y.E. (2021). Phosphorylation of SMURF2 by ATM exerts a negative feedback control of DNA damage response. The Journal of Biological Chemistry, 295(52), 18485-18493.

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Phospho-Antibody Detection Protocol

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Antibodies to phospho-AKT (Ser473) (1:5000, #4060), pan-AKT (1:2000, #9272), GST (1:3000, #2624), HA (1:3000, #3724), phospho-JNK(T182/Y185) (1:2000, #4668), pan-JNK (1:2000, #9252), phospho-AKT substrate (1:5000, #9614) and GAPDH (1:5000, #5174) were from Cell Signaling Technology. Antibody to HIP-55 (sc-366772, #1:2000) and 14-3-3 (1:2000, #sc-629) was from Santa Cruz Biotechnology. Antibodies to Flag (1:5000, #F3165) and Anti-Flag M2 affinity gel (#A2220) were from Sigma- Aldrich. Antibodies to GPX4 (1:3000, #ab125066) were from Abcam. Anti-phospho-HIP-55 (phosphorylated at S269) polyclonal antibodies (1:1000) were generated in rabbits against the synthesized phosphopeptide (KERAMpSTTS). Anti-phospho-HIP-55 (phosphorylated at T291) polyclonal antibodies (1:1000) were generated in rabbits against the synthesized phosphopeptide (FLQKQLpTQPE). Glutathione-Sepharose 4B was from GE Healthcare. Collagenase II (#1148090), DAPI (#D9542) and Hoechst (#94403) were from Sigma-Aldrich. RSL3 (#HY-100218A), Fer-1 (#HY-100579) and MK-2206 (#HY-10358) were from MedChemExpress.

Jiang Y., Qiao Y., He D., Tian A, & Li Z. (2023). Adaptor protein HIP-55-mediated signalosome protects against ferroptosis in myocardial infarction. Cell Death and Differentiation, 30(3), 825-838.

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