ApoE-KO mice were originally obtained from Jackson laboratory (Bar Harbor, ME, USA). Forty 12-week-old-male mice were bred at the animal center of National Yang-Ming University. The study followed the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85–23, revised 1996) and all experimental procedures were approved by the Animal Care and Utilization Committee of National Yang-Ming University, Taipei, Taiwan. After one week on a commercial mouse chow diet, the mice were randomly allocated to one of five groups (n = 8). The control group was given normal laboratory mouse diet ad libitum and 1.1% ethanol in PBS (150 mM NaCl, 20 mM sodium phosphate, pH 7.4) by gavage every day. The other four groups were fed the same normal diet ad libitum plus 200 mg/kg of DHA, EPA, AA, or LA in 1.1% ethanol/PBS every day by gavage. After 10 weeks on the diet, the mice were fasted overnight, and their body weight was recorded. The mice were then euthanized and blood and liver samples were collected at the end of the experiment. The blood was centrifuged at 12000 g for 15 min and the plasma supernatant was then stored at −35°C until analysis. Liver tissues were harvested, washed with ice-cold isotonic saline, and stored at −80°C until use.
Apoe ko mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
ApoE KO mice are genetically modified mice that lack the apolipoprotein E (ApoE) gene. This gene is involved in the regulation of cholesterol and lipid metabolism. ApoE KO mice are commonly used in research to study atherosclerosis, lipoprotein metabolism, and other cardiovascular and neurological disorders.
Automatically generated - may contain errors
Lab products found in correlation
Carboxymethylcellulose sodium salt, by Merck Group (1 mentions)
Laser doppler imager, by Moor Instruments (1 mentions)
Xylazine, by Bayer (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
Lithium heparin tube, by BD (1 mentions)
Sudan 4, by Merck Group (1 mentions)
Td 88137, by Inotiv (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
C57bl 6, by Jackson ImmunoResearch (1 mentions)
Bp 98a l, by Softron (1 mentions)
Lysm cre mice, by Jackson ImmunoResearch (1 mentions)
Apoe3, by Taconic Biosciences (1 mentions)
Cholesterol, by Research Diets (1 mentions)
19 protocols using apoe ko mice
1
Dietary Fatty Acid Effects in ApoE-KO Mice
2
Talin Modulator Enhances Arterial Blood Flow
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were approved by the Institutional Animal Care and Use Committee of Korea University College of Medicine (KOREA-2017-0126). Male, 10-week-old ApoE KO mice (#002052, The Jackson Laboratory, Bar Harbor, ME, USA) were anesthetized by intraperitoneal (IP) injection of ketamine (80 mg/kg; Yuhan, Seoul, Korea) and xylazine (8 mg/kg; Bayer, Leverkusen, Germany). After a groin incision, the left femoral arteries were injured by passing a sterile wire followed by ligation. ApoE KO mice were randomly assigned to the following 3 groups; sham (subjected to the procedure alone; n=5), vehicle control (DMSO; n=5), and talin modulator (5 mg/kg; n=6). The talin modulator and an equivalent volume of DMSO were prepared in 0.5% carboxymethylcellulose sodium salt (C5678, Sigma-Aldrich) for administration via oral gavage daily for 28 days. The mice were anesthetized at 7, 14, and 28 days post-procedure and the blood flow in the ventral side was analyzed using a laser Doppler imager (Moor Instruments, Devon, UK).
3
Hypoxanthine Ameliorates Atherosclerosis in ApoE Knockout Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seven‐week‐old male Apoe KO mice were obtained from the Jackson Laboratories (Bar Harbor, ME, USA). C57BL/6 wild‐type (WT) mice were obtained from Samtako (O‐san, Korea). Animal experiments were approved by the Animal Care and Use Committee of Kyungpook National University and conformed to the Guide for the Care and Use of Laboratory Animals (NIH, Bethesda, MD, USA). Animals were fed with a high‐fat, high‐cholesterol diet (Research Diets, diet D12336; New Brunswick, NJ, USA) containing 16.0% fat, 1.25% cholesterol and 0.5% sodium cholic acid for 12 weeks. Fourteen WT mice were randomized into the hypoxanthine‐non‐treated (WT, n = 6) and ‐treated groups (WT‐Hx, n = 8). Another 14 Apoe KO mice were randomized into the hypoxanthine‐non‐treated (Apoe KO, n = 6) and ‐treated groups (Apoe KO‐Hx, n = 8). After 4 weeks of high‐fat diet feeding, hypoxanthine was given intraperitoneally (200 mg/kg) daily for 8 weeks. At the end of in vivo experiments (12 weeks), the animals were killed under anaesthesia breathing 1.5% isofluorane on a mask, the aortae were excised and analysed histologically. Liver was snap frozen in liquid nitrogen and stored in −80°C for lipid measurements. The blood was harvested for serum chemistry tests.
4
Genetic Knockouts and Viral Injections in Mice
5
HDL Nanoparticle Therapy for Atherosclerosis
6
ApoE KO Mice Experimental Procedures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the in vivo experimental procedures carried out in this study were performed under the principle for replacement, refinement, or reduction (the 3Rs) and in accordance with the Directive 2010/63/EU of the European Parliament and were approved by the Institutional Animal Care and Use Committee of IIS-Fundación Jimenez Diaz and Community of Madrid (PROEX 116/16 and 217/19). Male Apolipoprotein E knockout (ApoE KO) mice (Jackson Laboratory, Bar Harbor, ME, USA) were housed in ventilated cages (2–3 mice per cage) with usual bedding material and environmental enrichment in a conventional temperature-controlled room (20–22 °C) with 12 h light/dark cycle and free access to water and standard food.
7
Atherosclerosis Imaging in ApoE-KO Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For biodistribution and in vivo specificity test, C57BL/6 mice (female, 7 week old, 19–22 g weight, from Charles River Laboratories) were used. Adult ApoE-KO mice (female and male, 40–48 weeks old, 25–32 g weight, from Jackson laboratory) which are known to develop extensive atherosclerotic lesions were used for in vivo and ex vivo imaging studies. Age-matched (female and male) C57BL/6 mice were used as controls. All mice were fed a normal chow diet. Experiments were approved by the local animal care committee and were in accordance with the German Animal Welfare Act.
8
ApoE-KO Mice Atherosclerosis Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were performed per the Institutional Animal Care and Use Committee (IACUC) Guidelines and Authorization for the Use of Laboratory Animals and were approved by the IACUC of Temple University School of Medicine. ApoE-KO mice in a wild-type (WT, C57BL/6) background were obtained from the Jackson Laboratory (Bar Harbor, ME). ApoE-KO mice were weaned at three weeks of age and given surgery and a high-fat diet (HFD) at 9 –10 weeks.
9
Generation and Use of ApoE-KO/PGC-1α Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Homozygous ApoE-KO mice and heterozygotes PGC-1α mice were crossbred and backcrossed into a murine ApoE-KO background. ApoE-KO mice and homozygous ApoE-KO/heterozygotes PGC-1α-b mice (ApoE-KO/PGC-1α mice) were obtained, and 16–20-week-old male offspring were used in experiments. All mice were maintained on a C57BL6/J background. ApoE-KO mice were obtained from The Jackson Laboratory (Bar Harbor, ME, USA)59 (link). The methods for generation of PGC-1α mice were described previously14 (link). The promoter for human α-skeletal actin, provided by Drs E. C. Hardeman and K. L. Guven (Children’s Medical Research Institute, Australia) was used to express PGC-1α-b in skeletal muscle. Animals were housed in groups of 5 mice per cage in a room with a 12-hour light/dark cycle at 22 °C and provided with standard mouse chow (CE-2, CREA Japan Inc., Tokyo, Japan) and drinking water ad libitum. Mice were cared for according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals (https://www/ncbi.nlm.nih.gov/books/NBK54050/) and our institutional guidelines. All animal experiments were approved by the Institutional Animal Care and Use Committee of the University of Shizuoka (number 165123).
10
Angiotensin II-Induced Aortic Aneurysm in ApoE-Deficient Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All procedures were approved by the Animal Care and Use Committee, Kyushu University and conducted in accordance with the institutional guidelines. ApoEKO mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). Development of AAA was induced as described previously15 (link), 16 (link)) with minor modifications17 (link)). Briefly, ApoEKO mice (12 week old male mice) were fed a HFD and administered Ang II for 4 weeks via osmotic mini pumps implanted subcutaneously. Osmotic mini pumps were implanted under anesthesia by intraperitoneal injection of ketamine (100 mg/kg) and xylazine (10 mg). At 4 weeks after the operation, the systolic blood pressure (SBP) and heart rate (HR) were measured with the tail-cuff method in a conscious state (BP-98A-L, Softron, Tokyo, Japan). Teneligliptin was given orally through drinking water and started 1 week before HFD and Ang II treatment (DPP-4i group). The approximate intake of teneligliptin was 30 mg/kg/day. The control (CTRL) group was given HFD and Ang II. The normal group was fed a normal chow.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!