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Ab131490

Manufactured by Abcam
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Ab131490 is a product offered by Abcam. It is a piece of laboratory equipment, but a detailed description is not available at this time.

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Lab products found in correlation

Ab53290, by Abcam (2 mentions) Ab52894, by Abcam (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions) Cy1109, by Cell Applications (1 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions) Ab40815, by Abcam (1 mentions) Ab2910, by Abcam (1 mentions) Ab32121, by Abcam (1 mentions) Ab189524, by Abcam (1 mentions) Tbs t, by Cell Signaling Technology (1 mentions) Rabbit anti perk, by Thermo Fisher Scientific (1 mentions) Rabbit anti p akt, by Cell Signaling Technology (1 mentions) Immobilon western, by Merck Group (1 mentions) Sc 47778, by Santa Cruz Biotechnology (1 mentions) Ab182858, by Abcam (1 mentions) Ab32503, by Abcam (1 mentions) Ab133459, by Abcam (1 mentions) Ab214430, by Abcam (1 mentions) Ab76132, by Abcam (1 mentions) Ab184787, by Abcam (1 mentions)

4 protocols using ab131490

1

Immunohistochemical Profiling of Breast Markers

Cited 1 time
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Immunohistochemical staining of TMA section was performed as previously described49 (link). Rabbit polyclonal antibodies for ERα (BS1114), ERβ (BS8465), PR (BS1766) and EGFR (BS1533) were purchased from Bioworld Technology (Minnesota, USA). Rabbit polyclonal antibody for ERα36 (CY1109) was purchased from Cell Applications (San Diego, CA, USA). Rabbit polyclonal antibody for HER2 (ab131490) was purchased from Abcam (Cambridge, MA, USA). These rabbit polyclonal antibodies were used as primary antibodies at 1:100 dilution and biotinylated goat-anti-rabbit IgG (ZB-2010, Zhongshan Golden Bridge Biotechnology, China) was used as a secondary antibody at 1:500 dilution.
Dai Y.J., Qiu Y.B., Jiang R., Xu M., Zhao L., Chen G.G, & Liu Z.M. (2017). Concomitant high expression of ERα36, EGFR and HER2 is associated with aggressive behaviors of papillary thyroid carcinomas. Scientific Reports, 7, 12279.
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2

Immunoblot Analysis of Signaling Proteins

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Whole-protein extracts from control or treated JIMT1 cells were obtained as previously described by cell scraping at 4 °C in a radioimmunoprecipitation assay buffer.20 (link) Following electrophoresis and transfer to nitrocellulose membranes (Thermo Fisher Scientific, IB23001), the blots were incubated in 5% w/v bovine serum albumin (BSA, Sigma, A7030) in Tris-buffered saline buffer-Tween (TBS-T, Cell Signaling Technology, 9997S) and probed with mouse anti-β-actin 1:20 000 (Sigma, A1978), rabbit anti-HER2 1:800 (Abcam, ab131490), rabbit anti-pHER2 1:800 (Abcam, ab53290), rabbit anti-EGFR 1:1000 (Abcam, ab52894), rabbit anti-pEGFR 1:500 (Abcam, ab40815), rabbit anti-HER3 1:500 (Abcam, ab32121), rabbit anti-pHER3 1:2500 (Abcam, ab76469), rabbit anti-AKT 1:1000 (Cell Signaling, 9272), rabbit anti-pAKT 1:2000 (Cell Signaling, 4060), mouse anti-ERK 1:1000 (Thermo Fisher Scientific, 14-9108-82), rabbit anti-pERK 1:500 (Thermo Fisher Scientific, 700012), rabbit anti-CD44 1:1000 (Abcam, ab189524), and rabbit anti-CAV1 1:500 (Abcam, ab2910) antibodies. After the antibodies were incubated and washed, the membranes were incubated with IRDye800CW anti-Rabbit (925–32211) or anti-Mouse (925–32210) IgG 1:15 000 (LI-COR Biosciences) and imaged on the Odyssey Infrared Imaging System (LI-COR Biosciences) followed by densitometric analysis using Fiji software (https://imagej.net/Fiji).
Pereira P.M., Ragupathi A., Shmuel S., Mandleywala K., Viola N.T, & Lewis J.S. (2019). HER2-Targeted PET Imaging and Therapy of Hyaluronan-Masked HER2-Overexpressing Breast Cancer. Molecular pharmaceutics, 17(1), 327-337.
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3

Western Blot Analysis of NRG-1 and ErbB Signaling

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Proteins from cultured MASMCs were prepared as previously described15 (link). Proteins were separated on 8%, 10% or 12% SDS-PAGE gels, and transferred to PVDF membranes. The membranes were blocked with 5% milk in TTBS for 2 h at room temperature and incubated with primary antibodies overnight at 4 °C. Antibodies are as follows: anti-NRG-1 (1:500, sc-348, santa), anti-NRG-1-ICD (1:500, sc-393009, santa), anti-Cleaved-caspase-3 (1:5000, ab214430, abcam), anti-caspase-3 (1:2000, ab184787, abcam), bax (1:1000, ab32503, abcam), bcl-2 (1:2000, ab182858, abcam), p-ErbB2 (1:500, ab47262, abcam), ErbB2 (1:500, ab131490, abcam), p-ErbB3 (1:1000, ab133459, abcam), ErbB3 (1:100, ab5470, abcam), p-ErbB4 (1:1000, ab76132, abcam), ErbB4 (1:1000, ab76303, abcam) and anti-β-actin (1:1000, sc-47778, Santa Cruz). The membranes were washed, incubated with the HRP-conjugated secondary antibodies for 1 h at room temperature, treated with the Immobilon™ Western (Millipore) and detected by ECL (enhanced chemiluminescence) BIO-RAD (721BR10829). All experiments were replicated three times.
Sun Y., Zhang S., Yue M., Li Y., Bi J, & Liu H. (2019). Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis. Cell Death & Disease, 10(5), 362.
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4

Protein Expression Analysis in PDX Models

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Whole-protein extracts from PDXs were obtained in radioimmunoprecipitation assay buffer as previously described (25 (link)). After electrophoresis and transfer to nitrocellulose membranes (IB23001; Thermo Fisher Scientific), the blots were incubated in 5% w/v bovine serum albumin (A7030; Sigma) in Tris-buffered saline with polysorbate (9997S; Cell Signaling Technology) and probed with mouse anti-β-actin, 1:20,000 (A1978; Sigma); rabbit anti-HER2, 1:800 (ab131490; Abcam); rabbit anti-phosphorylated HER2 (anti-pHER2), 1:800 (ab53290; Abcam); rabbit anti-EGFR, 1:1,000 (ab52894; Abcam); rabbit anti-MET, 1:1,000 (ab51067; Abcam); and anti-phosphorylated MET (anti-pMET), 1:1,000 (ab68141; Abcam). After antibody incubation and washing, the membranes were incubated with the secondary antibodies IRDye 800CW anti-rabbit (925-32211) or anti-mouse (925-32210) IgG, 1:15,000 (LI-COR Biosciences), and imaged on the Odyssey Infrared Imaging System (LI-COR Biosciences), followed by densitometric analysis using Fiji software (https://imagej.net/Fiji) (32 (link)).
Pereira P.M., Norfleet J., Lewis J.S, & Escorcia F.E. (2021). Immuno-PET Detects Changes in Multi-RTK Tumor Cell Expression Levels in Response to Targeted Kinase Inhibition. Journal of Nuclear Medicine, 62(3), 366-371.
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