Human ifn α elisa

Manufactured by R&D Systems

Sourced in France, United Kingdom

The Human IFN-α ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interferon alpha (IFN-α) levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Opteia human tnf elisa kit, by BD (1 mentions)

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ELISAs (144 citations) IFN-α (75 citations) Human IFN-α ELISA kit (2 citations)

3 protocols using human ifn α elisa

Cytokine Secretion Quantification by ELISA

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Supernatant from the cells were collected and cytokine secretion was measured by ELISA and Luminex assays. The secretion of TNF and IFN-α was quantified by ELISA using an OptEIA human TNF ELISA Kit (Becton Dickinson) and human IFN-α ELISA (R&D Systems; Lille, France) following the manufacturer’s instructions.

Tran T.T., Tran T.H, & Kremer E.J. (2021). IgG-Complexed Adenoviruses Induce Human Plasmacytoid Dendritic Cell Activation and Apoptosis. Viruses, 13(9), 1699.

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Cytokine Profiling in Cell Culture

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The concentrations of cytokines in culture media were evaluated using human IL-6 uncoated ELISA (88–7066), human TGF-β uncoated ELISA (88–50390), human SDF-1 (CXCL12) ELISA (EHCXCL12A), and human LIF ELISA (BMS242) from Invitrogen, human IFNα ELISA (41100–1, R&D Systems) and using human cytokine ELISA plate array I (EA-4001, Signosis), according to the manufacturer’s instructions.

Koikawa K., Kibe S., Suizu F., Sekino N., Kim N., Manz T.D., Pinch B.J., Akshinthala D., Verma A., Gaglia G., Nezu Y., Ke S., Qiu C., Ohuchida K., Oda Y., Lee T.H., Wegiel B., Clohessy J.G., Sanatagata S., Wulf G.M., Hidalgo M., Muthuswamy S., Nakamura M., Gray N.S., Zhou X.Z, & Lu K.P. (2021). Targeting Pin1 Renders Pancreatic Cancer Eradicable by Synergizing with Immunochemotherapy. Cell, 184(18), 4753-4771.e27.

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Quantifying Interferon Levels by ELISA

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All collected samples were centrifuged at 1500 rpm for 10 min at 4 °C, and the cell-free supernatant was immediately stored at -80 °C until the experiment was performed. The concentrations of IFN-α and IFN-β were measured by “sandwich” enzyme-linked immunosorbent assay (ELISA) using commercially available kits, namely, human IFN-α ELISA (R&D, UK) and human IFN-β ELISA (R&D, UK), according to the manufacturer’s instructions. The results were normalized to the amount of conditioned media and expressed as pg/ml.

Song J., Hu Y., Li J., Zheng H., Wang J., Guo L., Shi H, & Liu L. (2017). Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication. Archives of Virology, 163(1), 135-144.

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