Disodium tetraborate decahydrate

Pumpkin seeds (Cucurbita pepo L.) were provided by Sanxin Company (Ürümqi, Xinjiang, China). Reagents used in this study were of analytical grade unless otherwise specified. N-hexane was purchased from InnoChem Science and Technology Co., Ltd. (Beijing, China). Disodium hydrogen phosphate dodecahydrate, sodium phosphate dibasic dihydrate, disodium tetraborate decahydrate, O-phthalic aldehyde (OPA), dithiothreitol (DTT), serine, folin-phenol, sodium carbonate, trichloroacetic acid (TCA), absolute ethyl alcohol, sodium chloride, sodium hydroxide, calcium chloride, potassium chloride, sodium bicarbonate, and magnesium chloride, were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Coomassie Blue Fast Staining Solution G250, Bovine Serum Albumin (BSA), and Phosphate-Buffered Saline (PBS) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

Allopurinol tablets were procured from Shimao Tianjie Pharmaceutical (Jiangsu) Co., Ltd. Carboxymethylcellulose sodium, boric acid (Guaranteed reagent), disodium tetraborate decahydrate (Analytical Reagent), perchloric acid (Analytical Reagent), and acetic acid (Analytical Reagent) were all acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Potassium oxonate and adenine were sourced from Shanghai Macklin Biochemical Technology Co., Ltd. Yeast extract and 0.9% sodium chloride injection were purchased from Shanghai Chunshi Biotechnology Co., Ltd. (Shanghai, China) and Kelun Pharmaceutical Co., Ltd. (Sichuan, China), respectively. High-performance liquid chromatography (HPLC) grade methanol was obtained from TEDIA Company Inc (USA).