Total RNA was extracted from the frozen tissues using TRIzol reagent (Invitrogen), and a quantitative real-time PCR was performed using a StepOnePlus Real-time PCR System and the StepOne Software program (Applied Biosystems), as described previously [6 (link)]. The real-time PCR protocol consisted of one cycle at 95 °C for 20 s followed by 40 cycles at 95 °C for 1 s and 60 °C for 20 s using the primers for Nppb (Mm01255770_g1; Applied Biosystems), Ppara (Mm00440939_m1; Applied Biosystems), and Ppargc1 (Mm01208835_m1; Applied Biosystems). The transcriptional levels were determined using the ΔΔCt method with normalization to GAPDH (Mm99999915_g1; Applied Biosystems).
Mm01208835 m1
Manufactured by Thermo Fisher Scientific
The Mm01208835_m1 is a laboratory equipment product from Thermo Fisher Scientific. It serves as a core function for specific tasks within the laboratory setting. Due to the technical nature of the product, a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Stepone software program, by Thermo Fisher Scientific (1 mentions)
Mm99999915 g1, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions)
Mm00441242 m1, by Thermo Fisher Scientific (1 mentions)
Mm00443258 m1, by Thermo Fisher Scientific (1 mentions)
Mn03302249 g1, by Thermo Fisher Scientific (1 mentions)
Mm00515664 m1, by Thermo Fisher Scientific (1 mentions)
Mm01244861 m1, by Thermo Fisher Scientific (1 mentions)
2 protocols using mm01208835 m1
1
Quantitative Real-Time PCR Analysis of Gene Expression
2
Gene Expression Analysis in Tissues
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Polymerase chain reaction (PCR) for tissues was performed as described previously [24 (link)] using the primers for URAT1 (Applied Biosystems, Mm01236822_m1), UCP1 (Applied Biosystems, Mm01244861_m1), PGC1α (Applied Biosystems, Mm01208835_m1), Dio2 (Applied Biosystems, Mm00515664_m1), chemokine ligand 2 (Ccl2) (Applied Biosystems, Mm00441242_m1), tissue necrosis factor α (TNFα) (Applied Biosystems, Mm00443258_m1), and GAPDH (Applied Biosystems, Mn03302249_g1).
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