Seven-week-old male C57BL/6N mice (Samtako, Daejeon, South Korea) were adapted to the facility for 1 week before study. The mice were randomly grouped into the following groups (n = 8 for each group): control (Con), cisplatin alone (CP), and SRT1720 in combination with cisplatin (CP+SRT1720). To induce AKI, mice were intraperitoneally injected with cisplatin (15 mg/kg in 0.9% normal saline). Control mice were intraperitoneally injected with an equal volume of the vehicle. To investigate the effects of SRT1720 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) on cisplatin-induced AKI, mice were intraperitoneally injected with SRT1720 (20 mg/kg) [16 (link),17 (link)] for 3 days. The treatment with SRT1720 started 1 h before cisplatin injection. Mice were sacrificed 72 h after cisplatin injection. All animal experiments were performed according to the animal protocols approved by the Institutional Animal Care and Use Committee of the Catholic University of Daegu (DCIAFCR-180809-13-Y).
Srt1720
Manufactured by Santa Cruz Biotechnology
Sourced in United States
SRT1720 is a selective and potent small-molecule agonist for the SIRT1 deacetylase enzyme. It acts by directly binding and activating SIRT1, which is involved in the regulation of various cellular processes. The core function of SRT1720 is to serve as a research tool for studying the role and mechanisms of SIRT1 in different biological systems.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6n mice, by Samtako Bio (1 mentions)
Forskolin, by Merck Group (1 mentions)
Wortmannin, by Merck Group (1 mentions)
Sirt1, by Cell Signaling Technology (1 mentions)
Ex 527, by Merck Group (1 mentions)
Doxorubicin adr, by Merck Group (1 mentions)
Ac h3, by Santa Cruz Biotechnology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Insulin, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Ex527, by Bio-Techne (1 mentions)
Penicillin, by Minerva Biolabs (1 mentions)
On targetplus smartpool sirna against mouse, by Horizon Discovery (1 mentions)
Venor gem mycoplasma detection kit, by Minerva Biolabs (1 mentions)
D 001810, by Horizon Discovery (1 mentions)
Mg132, by Merck Group (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg hrp sc 2004, by Santa Cruz Biotechnology (1 mentions)
Primescript first strand cdna synthesis kit, by Takara Bio (1 mentions)
Mouse anti human β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
5 protocols using srt1720
1
Cisplatin-Induced Acute Kidney Injury in Mice
2
Sirt1-mediated Metabolic Regulation Analysis
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SRT1720 was supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Doxorubicin (ADR), EX527, wortmannin, insulin, H‐89 and forskolin were supplied by Sigma (St. Louis, MO, USA). Compound C was obtained from Calbiochem (La Jolla, CA, USA). Antibodies were purchased from the following vendors: SIRT1, p‐AMPK (Ser485), H3, p‐AKT, AKT p‐PKA (Thr197) and PKA (Cell Signaling Technology, Danvers, MA, USA); and Ac‐H3, p53, p21, p16, telomerase reverse transcriptase (TERT), SMP‐30 and β‐actin (Santa Cruz Biotechnology).
3
Glioma and Microglia Coculture Assay
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Microglia BV2 (gift from G. Brown, University of Cambridge), glioma C6 (ATCC® CCL-107™) and glioma GL261 (DSMZ ACC 802, gift from R. Glass, University hospital of Munich) rodent cell lines have been used in this study. Cells were regularly tested with Venor™GeM mycoplasma detection kit (Minerva Biolabs) and maintained in DMEM medium supplemented with penicillin (100 μg/ml), streptomycin (100 μg/ml) and 10% fetal calf serum (FCS) (all from Gibco™ ThermoFisher Scientific). Segregated cocultures were performed using a transwell system using the above mentioned medium with FCS reduced to 5%. Transfection of BV2 cells was carried out with Lipofectamine® 3000 (Invitrogen, ThermoFisher Scientific) following the manufacturer's recommendation. Predesigned ON-TARGETplus SMARTpool siRNA against mouse SIRT1 (L-048962-09) and mouse MOF (L-049440-05) as well as non-targeting control (D-001810) were purchased from Dharmacon. BV2 cells were treated with 50 µM EX527 (Tocris Bioscience) for 4 h, 5 µM SRT1720 (Santa Cruz Biotechnology) for 17 hours, or 250 nM MG132 (Sigma Aldrich) for 4 hours.
4
AICAR Modulates AMPK and SIRT1 Signaling
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Immortalized mouse myoblast C2C12 cells (CRL-1772) were purchased from the American Type Culture Collection (Manassas, VA, USA). 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), 6-[4-(2-piperidin-1-ylethoxy)phenyl]-3-pyridin-4-ylpyrazolo[1,5-a]pyrimidine (CC), NA, and RP were obtained from Sigma–Aldrich (St. Louis, MO, USA). Mouse antihuman AMPK (#2793), rabbit antihuman phospho-AMPK (#2535), and rabbit antihuman phospho-SIRT1 (#2314) were purchased from Cell Signaling Technology (Danvers, MA, USA). SRT1720, protein A/G agarose, rabbit antihuman SIRT1 (Sc-15404), rabbit antihuman PGC-1α (Sc-13067), anti-acetylated lysine (Ac-Lys; Sc-32268), mouse antihuman β-actin (Sc-47778), goat antimouse IgG–horseradish peroxidase (HRP; Sc-2005), and goat antirabbit IgG-HRP (Sc-2004) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The enhanced chemiluminescence (ECL) Plus western blotting substrate was purchased from Pierce Biotechnology (Rockford, IL, USA). TRIzol was purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). The PrimeScript first-strand cDNA synthesis kit was purchased from Takara Bio (Shiga, Japan). FastStart universal SYBR Green master was obtained from Roche Applied Science (Basel, Switzerland).
5
Fetal Bovine Serum-Based Cell Culture Protocol
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Fetal bovine serum (FBS), medium 199 (M199), and trypsin-EDTA were obtained from GIBCO (Grand Island, NY). EDTA, penicillin, and streptomycin were obtained from Sigma (St. Louis, MO). Terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) staining kits were obtained from Boehringer Mannheim (Mannheim, Germany). The superoxide dismutase activity assay kit was purchased from Calbiochem (San Diego, CA). DCF-AM was obtained from Molecular Probes (Eugene, OR). 5,58,6,68-tetraethylbenzimidazolcarbocyanine iodide (JC-1) and anti-active caspase 3 were obtained from BioVision (Palo Alto, CA). Anti-vascular cell adhesion molecule-1 (VCAM-1), anti-intercellular adhesion molecule (ICAM), and anti-E-selectin were purchased from R&D Systems (Minneapolis, MN). SRT1720, anti-SIRT1 and anti-β-actin were obtained from Santa Cruz Biotechnology (CA, USA). Anti-acetyl-p53 and anti-p53 were obtained from Cell Signaling (MA, USA).
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