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Cyppa

Manufactured by Merck Group
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Sourced in France

CyPPA is a lab equipment product manufactured by Merck Group. It is a selective activator of the calcium-activated potassium channel, SK3. The product's core function is to modulate potassium channel activity for research applications.

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Lab products found in correlation

Be12 702f, by Lonza (2 mentions) Ns309, by Merck Group (2 mentions) Lncap, by American Type Culture Collection (1 mentions) Crl 3314, by American Type Culture Collection (1 mentions) Lncap, by Lonza (1 mentions) Cvf5vf00 01, by Eurobio Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Sodium selenite, by Merck Group (1 mentions) β mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Ns309, by Bio-Techne (1 mentions) 6 well suspension plates, by Greiner (1 mentions) Cvfsvf00 01, by Eurobio Scientific (1 mentions) Apamin, by Merck Group (1 mentions) Sch23390, by Merck Group (1 mentions) Haloperidol, by Merck Group (1 mentions) 6 ohda, by Merck Group (1 mentions) Raclopride, by Merck Group (1 mentions)

6 protocols using cyppa

1

Prostate Cancer Cell Lines and Drug Treatments

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PCa cells lines LNCaP (CRL-1740), and second-generation LNCaP subline: C4-2 (CRL-3314) and C4-2B (CRL3315) (B for Bone Metastatic) [16 (link)] were purchased from American Type Culture Collection (ATCC). LNCaP cells were cultured in Roswell Park Memorial Institute medium containing D-Glucose (4.5 g/L), HEPES Buffer (2383 g/L), L-Glutamine, Sodium Bicarbonate (1.5 g/L), sodium pyruvate (110 mg/L) (RPMI, A1049101, Lonza, Levallois-Perret, France) and supplemented with 10% fetal bovine serum (FBS) (CVF5VF00-01, Eurobio, Les Ulis, France) and 1% penicillin-streptomycin (PS). C4-2 and C4-2B cells are cultured in RPMI medium (BE12-702F, Lonza, Levallois-Perret, France) supplemented with 5% FBS and 1% PS. Cells were maintained in a 37 °C humidified incubator with 5% CO2.
The non-steroidal AR inhibitor, Enza (PHB00235) and the SK3 positive modulator CyPPA (Cyclohexyl-[2-(3,5-dimethyl-pyrazol-1-yl)-6-methyl-pyrimidin-4-yl]-amine) were purchased from Sigma-Aldrich (St-Quentin Fallavier, France). 1-Ohexadecyl-2-O-methyl-sn-glycero-3-lactose (Ohmline) was synthetised as previously described [17 (link)]. For one-week treatments, cells were treated 3 times per week with Enza, Enza+Ohmline or CyPPA.
Bery F., Cancel M., Guéguinou M., Potier-Cartereau M., Vandier C., Chantôme A., Guibon R., Bruyère F., Fromont G, & Mahéo K. (2021). Zeb1 and SK3 Channel Are Up-Regulated in Castration-Resistant Prostate Cancer and Promote Neuroendocrine Differentiation. Cancers, 13(12), 2947.
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2

Embryoid Body-Based Differentiation Using bSF Medium

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Cells were mechanically detached from the conventional 6-well plate culture (Nunc) using a cell scraper and transferred to 6-well suspension plates (Greiner) in a well-per-well scheme for EB-based differentiation in basic serum-free (bSF) medium (Kempf et al., 2011 (link), Xu et al., 2008 (link)) consisting of DMEM (Gibco) supplemented with 1% non-essential amino acids (Gibco), 2 mmol/L L-glutamine (Gibco), 0.1 mmol/L β-mercaptoethanol (Gibco), 5.6 mg/L transferrin (Sigma-Aldrich), and 37.2 μg/L sodium selenite (Sigma-Aldrich). bSF medium was supplemented with 5 μM SB203580 (Graichen et al., 2008 (link)), a p38 MAPK inhibitor, from day 0 to day 6. The compounds 1-ethyl-2-benzimidazolinone (EBIO; 1 M stock solution; Tocris Bioscience or Institute of Organic Chemistry at Hannover University), CyPPA (15 mM stock solution, Tocris), and NS309 (3 mM stock solution, Tocris) were added at respective concentrations and timing as described in Results. DMSO (Sigma-Aldrich), the solvent of EBIO, CyPPA, and NS309, was applied in respective amounts in controls. Apamin was diluted in sterile H2O (Tocris, 0.1 mM stock solution). Medium was changed every 2–3 days. Before EB dissociation for endpoint analysis, cells were cultured for 24 hr in bSF medium.
Jara-Avaca M., Kempf H., Rückert M., Robles-Diaz D., Franke A., de la Roche J., Fischer M., Malan D., Sasse P., Solodenko W., Dräger G., Kirschning A., Martin U, & Zweigerdt R. (2017). EBIO Does Not Induce Cardiomyogenesis in Human Pluripotent Stem Cells but Modulates Cardiac Subtype Enrichment by Lineage-Selective Survival. Stem Cell Reports, 8(2), 305-317.
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3

Characterization of PC3 Cell Line for Cancer Research

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Human PCa PC3 (CRL-1435) cell line (American Type Culture Collection (ATCC)) was received in 2016–2018. This cell line was tested and authenticated by DNA fingerprinting by the ATCC. After reception, cells were amplified in order to make a large reserve of cryopreserved cells. Every 3 months, a new cryopreserved bulb was thawed and used for this study. PC3 cells were cultured in RPMI medium (BE12-702F, Lonza, Levallois-Perret, France), supplemented with 5% FBS (CVFSVF0001, Eurobio, Les Ulis, France) and 1% (v/v) penicillin–streptomycin in a 37 °C-humidified incubator, 5% CO2.
LA (L1876), EPA (17266), and PA (P5177) were from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Calcium channel inhibitors GSK7975A (GLXC03243) and Synta66 (GLXC03244) were from GLIXX Laboratories INC (Hopkinton, MA, USA). The SK3 activator, CyPPA, was purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). 1-Ohexadecyl-2-O-methyl-sn-glycero-3-lactose (Ohmline) was synthetized as previously described [28 (link)].
Bery F., Figiel S., Kouba S., Fontaine D., Guéguinou M., Potier-Cartereau M., Vandier C., Guibon R., Bruyère F., Fromont G, & Mahéo K. (2020). Hypoxia Promotes Prostate Cancer Aggressiveness by Upregulating EMT-Activator Zeb1 and SK3 Channel Expression. International Journal of Molecular Sciences, 21(13), 4786.
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4

Whole-cell patch-clamp of ion channels

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Whole-cell configuration was achieved in Ca2+-containing physiological saline bath solution (mm): NaCl 135, KCl 5, MgCl2 1.2, CaCl2 2, glucose 10, Hepes 10, pH 7.4 with Tris-base. The pipette solution contained (mm): KCl 135, CaCl2 0.012, MgATP 3, Na2GTP 0.1, creatine phosphate disodium 2.5, EGTA 0.1, glucose 10, Hepes 10, pH 7.2 with Tris-base. The KRB buffer solution contained (mM): NaCl 120, KCl 5, CaCl2 2, MgCl2 1 NaHCO3 25, d-glucose 5.5. All drugs and reagents including apamin and CyPPA (N-cyclohexyl-N-[2-(3,5-dimethyl-pyrazol-1-yl)-6-methyl-4-pyrimidinamine) were purchased from Sigma.
Lee H., Koh B.H., Peri L.E., Woodward H.J., Perrino B.A., Sanders K.M, & Koh S.D. (2022). Role of detrusor PDGFRα+ cells in mouse model of cyclophosphamide-induced detrusor overactivity. Scientific Reports, 12, 5071.
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5

Neuropharmacological Modulation of Ion Channels

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Bee venom (Apis mellifera), apamin (SK channel blocker), CyPPA (cyclohexyl-[2-(3,5-dimethyl-pyrazol-1-yl)-6-methyl-pyrimidin-4-yl]-amine; SK channel positive modulator), haloperidol (preferential dopamine D2 receptor antagonist), raclopride (dopamine D2 receptor antagonist), SCH23390 (R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5,-tetrahydro-1H-3-benzazepine; dopamine D1 receptor antagonist) and 6-OHDA were purchased from Sigma-Aldrich (Lyon, France). Drugs were prepared as follows: haloperidol in distilled water with a methylparaben and propylparaben solution and a drop of lactic acid (0.1 N), CyPPA in saline containing ethanol 0.1%, SCH23390 in distilled water and the other drugs in saline, added with 0.1% ascorbic acid for 6-OHDA and apomorphine.
Maurice N., Deltheil T., Melon C., Degos B., Mourre C., Amalric M, & Kerkerian-Le Goff L. (2015). Bee Venom Alleviates Motor Deficits and Modulates the Transfer of Cortical Information through the Basal Ganglia in Rat Models of Parkinson’s Disease. PLoS ONE, 10(11), e0142838.
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6

Vasodilator Drug Solubility Protocol

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The following drugs were used: acetylcholine chloride, phenylephrine hydrochloride, indomethacin, l-NAME, apamin, 1-[(2-chlorophenyl) diphenylmethl]-1H-pyrazole (TRAM-34), RN-1734, HC-067047, Pyr3, GSK1016790A, CyPPA, 1-EBIO, NS309, SKA-31, and levcromakalim (Sigma-Aldrich, St. Louis, MO). Indomethacin, TRAM-34, RN-1734, HC-067047, Pyr3, GSK1016790A, CyPPA, 1-EBIO, NS309, and SKA-31 were dissolved in dimethyl sulfoxide. levcromakalim was dissolved in ethanol. All other drugs were dissolved in distilled water. All drugs were further diluted 1000-fold in Krebs solution to give the final chamber concentrations.
Seki T., Goto K., Kiyohara K., Kansui Y., Murakami N., Haga Y., Ohtsubo T., Matsumura K, & Kitazono T. (2017). Downregulation of Endothelial Transient Receptor Potential Vanilloid Type 4 Channel and Small-Conductance of Ca2+-Activated K+ Channels Underpins Impaired Endothelium-Dependent Hyperpolarization in Hypertension. Hypertension (Dallas, Tex. : 1979), 69(1).
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