Ultrahydrogel 250 column

Manufactured by Waters Corporation

Sourced in United States

The Ultrahydrogel 250 column is a size exclusion chromatography column designed for the separation and analysis of macromolecules and small molecules in aqueous solutions. It features a hydrophilic, cross-linked polyhydroxylated polymer-based packing material that provides high-resolution separation over a wide molecular weight range.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Ultrahydrogel 250 (13 citations) Ultrahydrogel columns (13 citations) Ultrahydrogel 250 molecular exclusion column (3 citations) Ultrahydrogel 120 and 250 columns (2 citations) Ultrahydrogel TM 250 column (2 citations)

12 protocols using ultrahydrogel 250 column

Molecular Weight Determination of GLS Oligosaccharides

Check if the same lab product or an alternative is used in the 5 most similar protocols

The molecular weights (M_w) of the GLS oligosaccharides were determined by GPC (2695; Waters, USA) with an Agilent 1260 differential detector.^{6 (link)} The analytical system consisted of Ultra hydrogel 500 columns (7.8 × 300 mm; Waters, USA), Ultra hydrogel 250 columns (7.8 × 300 mm; Waters, USA), and Ultra hydrogel 120 columns (7.8 × 300 mm; Waters, USA), and connected in series by decreasing pore sizes. The mobile phase was 0.1 M in potassium nitrate and the flow rate was 1.0 mL min⁻¹. The column temperature was fixed at 40 °C. Standards and samples were filtered through a 0.22 μm syringe filter and the injection volume was 40 μL. The molecular weight was calibrated with a series of polyethylene glycol standards. The M_ws of the samples was determined by comparing the retention time with the standard curves.

Yang K., Zhang Y., Cai M., Guan R., Neng J., Pi X, & Sun P. (2020). In vitro prebiotic activities of oligosaccharides from the by-products in Ganoderma lucidum spore polysaccharide extraction. RSC Advances, 10(25), 14794-14802.

+ Open protocol

+ Expand

Comprehensive Polymer Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

¹H NMR spectra of the synthesized polymers were determined on a Bruker Avance III 400 MHz NMR spectrometer with D₂O as solvent, and the FTIR spectra were recorded on a Bruker Tensor-27 FT-IR spectrometer under strictly constant conditions in the region of 400 to 4000 cm⁻¹. The monomer conversions of VPA and PEGMA were calculated by simple mathematical expressions using relative peak integrations of the ¹H NMR spectra. The molecular weight and the polydispersity index (Đ = M_w/M_n) of the polymers were characterized by gel permeation chromatography (GPC) equipped with a Waters 2695 GPC with two Waters Ultrahydrogel 250 columns (7.8 mm × 300 mm) and Waters 2414 refractive index detector using NaNO₃ aqueous solution (0.1 mol L⁻¹, pH = 12) as the eluent and relative to the polyethylene glycol standards. The phosphorus contents in the synthesized polymers were detected by inductively coupled plasma-optical emission spectroscopy (ICP-OES, Spectro Blue Sop, German), with the incident frequency of 27 MHZ and power of 1400 W.

Li S., Zhou D., Shu X., Ran Q, & Yang Y. (2024). The synthesis and adsorption–dispersion properties of PPEGMA–PVPA copolymers in cement paste. RSC Advances, 14(22), 15812-15820.

+ Open protocol

+ Expand

Synthesis and Characterization of Dextrin-Colistin Conjugates

Check if the same lab product or an alternative is used in the 5 most similar protocols

Synthesis of dextrin-colistin conjugates. Dextrin-colistin conjugates, having 1, 2.5 and 7.5 mol% succinoylation of dextrin (Mw = 7,500 g/mol; degree of polymerization (DP) = 50), were synthesized using EDC and sulfo-NHS, puri ed by fast protein liquid chromatography (FPLC) using a HiLoad 16/600 Superdex 75 column and characterized according to previously optimized methods 11 (link) . Molecular weight was determined using a Viscotek TDA302 triple detector system, equipped with integrated refractive index (RI), viscometer and light scattering (LALS and RALS) detection and a Viscotek 2501 UV detector (210 nm). The column set consisted of an Ultrahydrogel guard column and two Ultrahydrogel 250 columns (7.8 mm X 300 mm) from Waters (Elstree, UK) in series, running at 30°C with PBS buffer eluent and a ow rate of 0.7 mL/min. Samples (300 µL) were injected into a 100 µL loop. A polyethylene oxide standard (Mw = 23,964, Mn = 23,502, IV = 0.404) from Malvern Panalytical (Malvern, UK) was employed for the calibration set up. The characteristics of dextrin-colistin conjugates used in these studies are summarized in Table S1.

Chiron E., Varache M., Stokniene J., Thomas D.W., & Ferguson E.L. (2021). A physicochemical assessment of the thermal stability of dextrin-colistin conjugates.

+ Open protocol

+ Expand

Molecular Weight Determination of Polysaccharides

Check if the same lab product or an alternative is used in the 5 most similar protocols

The molecular weight (Mw) distributions of the RBS and GSRBP were determined by using a HPGPC (Waerst e2695, USA) equipped with a refractive index detector (RID). Waters Ultrahydrogel™ 1000 column (7.8 × 300 mm) and Waters Ultrahydrogel™ 250 column (7.8 × 300 mm) were used in series. Sample solutions (20 μL) was injected and run with 0.1 mol/L NaNO₃ aqueous solution at 0.8 mL/min as mobile phase. The column-oven temperature was 40 °C. The standard curve was established by using Dextran for molecular weights (from Macklin, Mw: 4320 Da, 12,600 Da, 60,600 Da, 110,000 Da and 289,000 Da) as the standards. The molecular weight of each composition was calculated by contrast with the retention time of polysaccharides reference standard.

Han W., Chen H., Zhou L., Zou H., Luo X., Sun B, & Zhuang X. (2021). Polysaccharides from Ganoderma Sinense - rice bran fermentation products and their anti-tumor activities on non-small-cell lung cancer. BMC Complementary Medicine and Therapies, 21, 169.

+ Open protocol

+ Expand

Determining PACI-1 Molecular Weight

Check if the same lab product or an alternative is used in the 5 most similar protocols

The uniformity and molecular weight of PACI-1 were determined by HPLC (Agilent 1260 series, Agilent Technologies, USA) with an evaporative light-scattering detector (ELSD) and ultrahydrogel 250 column (7.8 × 300 nm, Waters Corp., USA). The injection volume was 20 μL of 2 mg/mL sample solution, the mobile phase was ultrapure water, the flow rate was 1 mL/min, and the column temperature was 35°C. The T-series dextran standard was used to construct the standard curve, which was used to determine the molecular weight of PACI-1 (3 (link)).

Zhuansun W., Xu J., Liu H., Zhao Y., Chen L., Shan S., Song S., Zhang H., Dong T., Zeng H, & Xu Q. (2022). Optimisation of the production of a selenium-enriched polysaccharide from Cordyceps cicadae S1 and its structure and antioxidant activity. Frontiers in Nutrition, 9, 1032289.

+ Open protocol

+ Expand

Determining Hyaluronan Molecular Weights

Check if the same lab product or an alternative is used in the 5 most similar protocols

Average molecular weights of the HA fractions were determined by HP-SEC using Ultrahydrogel 250 column (300 × 7.8 mm, 6 µm, pore size 250 Å; Waters, Milford, MA, USA). The mobile phase was 0.1 M Tris-HCl, pH 8.9 (1 mL/min). We used an Ultimate 3000 chromatograph (Dionex, Sunnyvale, CA, USA) equipped with a vacuum degasser, LPG-3400SD pump, column thermostat TCC-3000SD, and a spectrophotometric detector DAD-3000 operating at 240 nm. The molecular weights of the fractions were estimated by comparison with the retention times of polystyrene sulfonate standards (PSS Polymer Standards Service GmbH, Mainz, Germany).

Zykova M.V., Schepetkin I.A., Belousov M.V., Krivoshchekov S.V., Logvinova L.A., Bratishko K.A., Yusubov M.S., Romanenko S.V, & Quinn M.T. (2018). Physicochemical Characterization and Antioxidant Activity of Humic Acids Isolated from Peat of Various Origins. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(4), 753.

+ Open protocol

+ Expand

Synthesis and Characterization of PLGA-g-PEI Copolymer

Check if the same lab product or an alternative is used in the 5 most similar protocols

PLGA (800 mg, 200 μmole) was dissolved in 20 ml dried anhydrous DMF. N-hydroxysuccinimide (NHS, 27.6 mg, 240 μmole) and N,N′-Dicyclohexylcarbodiimide (DCC, 49.6 mg, 240μmole) were added to the reaction solution and this mixture was stirred for 2 hrs to activate the carboxylic end group of PLGA. The resulting precipitate, dicyclohexyl urea (DCU), was removed by filtration. bPEI (1.25 gm, 50 μmole) was dissolved in 20 ml dried DMF. The activated PLGA solution was added dropwise to the bPEI solution over 30 min, and then the mixture was allowed to react for 24 hrs at room temperature with stirring. Poly (lactide-co-glycolide)-g-poly (ethylenimine) (PgP) was purified by dialysis against deionized water using a membrane filter (MWCO=50,000), centrifuged at 5,000 rpm for 10 minutes to remove unreacted PLGA precipitate, and lyophilized. The structure of PgP was determined by FT-IR and ¹H- NMR (300 MHz, Bruker) using D₂O as a solvent. The molecular weight was determined by gel permeation chromatography (GPC, Waters, Milford, MA) using an Ultrahydrogel 250 column (7.8×300 mm) and guard column- 6×40 mm with water as the mobile phase. PgP solution (3 mg/ml, 20 μl) was injected by auto-injector and the flow rate was 0.7 ml/minute. A Waters 1525 HPLC pump and Waters 2414 Refractive Index Detector were used. Dextrans at molecular weights of 5, 12, 25, 50, and 80kDa were used as standards.

Gwak S.J., Nice J., Zhang J., Green B., Macks C., Bae S., Webb K, & Lee J.S. (2016). Cationic, amphiphilic copolymer micelles as nucleic acid carriers for enhanced transfection in rat spinal cord. Acta biomaterialia, 35, 98-108.

+ Open protocol

+ Expand

Characterizing Polymer Molecular Weights

Check if the same lab product or an alternative is used in the 5 most similar protocols

GPC was used to measure the molecular weights of parental and FA‐conjugated polymers. The Waters 1515 GPC system is equipped with an ultrahydrogel 250 column (Waters Corporation, MA) and a refractive index detector (Waters 2410). An aqueous solvent containing 0.1% trifloroacetic acid and 40% acetonitrile was used as the mobile phase. The mobile phase in the column was operated at a flow rate of 0.5 mL/min, and the column temperature was set at 35°C. Poly(2‐vinylpyridine) standards (MWs: 3,300, 7,600, 12,800, 35,000, and 70,000 Da) were employed for molecular weight (MW) calibration. All chromatograms were analyzed using the Waters Millennium 32 GPC software. To further investigate the MW range, the parental polymers were first dialyzed through a 3.5 kDa MWCO membrane to remove any unreacted monomers. Following this first step for 48 hr, the retentate was dialyzed through a 10 kDa MWCO membrane. Samples of retentates from the 3.5 and 10 kDa MWCO dialysis membranes were subjected to ninhydrin analysis which reports for reactive amine content. A calibration using glycine as a standard was used to determine the amine content of polymers, and the relative amounts of amines left behind in the retentates were compared to determine the MW range of the synthesized polymers.

Godeshala S., Nitiyanandan R., Thompson B., Goklany S., Nielsen D.R, & Rege K. (2016). Folate receptor‐targeted aminoglycoside‐derived polymers for transgene expression in cancer cells. Bioengineering & Translational Medicine, 1(2), 220-231.

+ Open protocol

+ Expand

SEC Analysis of CCP Molecular Properties

Check if the same lab product or an alternative is used in the 5 most similar protocols

The CCP average molecular weight (Mw) and polydispersivity index (Pi) were deter-mined by size exclusion chromatography (SEC) using an Agilent 1200 chromatographic system coupled with a G7162A Refractive Index Detector (RID) (Agilent Technologies, Santa Clara, CA, USA). Narrow pullulan standards in the 5−642 × 10³ g/mol range were used for the calibration curve (Waters Corporation, Milford, MA, USA). An Ultrahydrogel™ 2000 column (12 µm, 7.8 mm × 300 mm) and an Ultrahydrogel™ 250 column (6 µm, 7.8 mm × 300 mm) (Waters Corporation, Milford, MA, USA) were coupled in series and operated at a constant flow rate of 0.8 mL/min. The mobile phase consisted of a 0.1 M NaCl solution (w/v) containing 0.02% NaN₃ (w/v), filtered through a 0.45 µm PTFE membrane (Merck-Millipore, Milan, Italy). Columns and detector were maintained at 40 °C.
Samples were prepared by dissolving CCP or pullulan standards in 0.1 M NaCl (1 mg/mL final concentration) and filtered (cellulose microporous membrane filter, 0.45 mm, Merck-Millipore, Milan, Italy) before injection (injection volume 50 µL).

Ferron L., Milanese C., Colombo R., Pugliese R, & Papetti A. (2022). A New Polysaccharide Carrier Isolated from Camelina Cake: Structural Characterization, Rheological Behavior, and Its Influence on Purple Corn Cob Extract’s Bioaccessibility. Foods, 11(12), 1736.

+ Open protocol

+ Expand

Synthesis and Characterization of PEG-APV Conjugates

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mPEG_x-NHS (x = 2, 5, 10 or 30 kDa) polymers (0.01mmol) were reacted with APV-O-acetyl (0.03 mmol, 3 equiv) in DMF (4 mL) containing DIPEA (2%). The reaction mixtures were stirred at room temperature for 24 hr. The products were purified on Sephadex LH-20 (2 and 5 kDa conjugates) or Sephadex LH-60 column (10 and 30 kDa conjugates). The O-acetyl groups in PEG_X-APV-O-acetyl derivatives were removed by treating with HCl (0.1 N) at room temperature for 6 hr. The HCl was then neutralized with sodium bicarbonate and the solutions were lyophilized to obtain crude conjugates as white flakes. The conjugates were purified by size exclusion chromatography (SEC) on Waters Ultrahydrogel 250 column using water as mobile phase (flow rate: 0.8 ml/min) and characterized using MALDI-TOF-MS. The reaction yields were ~50% for all PEG conjugates. The retention times were: for the 2 kDa: R_t = 24 min, m/z observed 2,505.6 Da; 5 kDa: R_t = 23 min, m/z observed 5,625.6 Da; 10 kDa: R_t = 20 min, m/z observed 10,700.1 Da; 30 kDa: R_t = 17 min, m/z observed 31,098.7 Da.

Samizadeh M., Zhang X., Gunaseelan S., Nelson A.G., Palombo M.S., Myers D.R., Singh Y., Ganapathi U., Szekely Z, & Sinko P.J. (2016). Colorectal Delivery and Retention of PEG-Amprenavir-Bac7 Nanoconjugates - Proof of Concept for HIV Mucosal Pre-Exposure Prophylaxis. Drug delivery and translational research, 6(1), 1-16.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!