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Lipid peroxidation assay kit

Manufactured by Nanjing Jiancheng
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The Lipid peroxidation assay kit is a laboratory equipment designed to measure the level of lipid peroxidation in biological samples. It provides a quantitative assessment of malondialdehyde (MDA), a byproduct of lipid peroxidation, in the sample. The kit includes necessary reagents and a protocol to perform the assay.

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Lipid Peroxidation Kit (3 citations)

9 protocols using lipid peroxidation assay kit

1

Iron, Lipid Peroxidation, and Protein Quantification

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The tissue fluid was collected 24 h after reperfusion as described above for detecting iron and LPO levels by using an iron assay kit (Nanjing Jiancheng, China) and lipid peroxidation assay kit (Nanjing Jiancheng, China) according to the manufacturer's protocols. The total protein content was analyzed by the BCA protein detection kit (Beijing Soleibao Biotechnology, China).
Sun M., Liu M., Li Q., Zhang X., Liu S., Yang H., Yang L., Tian J., Mi W, & Ma Y. (2023). Cottonseed oil alleviates ischemic stroke injury by inhibiting ferroptosis. Brain and Behavior, 13(10), e3179.
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2

Lipid Peroxidation and GSH/GSSG Analysis

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The level of MDA was determined using a Lipid Peroxidation Assay Kit (A003-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
The level of GSH/GSSG was determined using GSH/GSSG Ratio Detection Assay Kit (A061-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
Zhao C.Q., Wang C., Liu M.M., Cao M., Peng J., Kong D.Q., Ren X.T., Liu R., Hai C.X, & Zhang X.D. (2023). Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis. Scientific Reports, 13, 6833.
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3

Evaluation of Lipid Peroxidation in Brain

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Lipid peroxidation (LPO) levels in lysate brain tissues and neurons were evaluated using the lipid peroxidation assay kit (Jiancheng, Nanjing, China), according to the manufacturer’s instructions. Furthermore, lipid ROS were assayed in neurons using the live-cell analysis reagent, BODIPY 581/591 C11 staining (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. Images were obtained by fluorescence microscopy (Olympus, Tokyo, Japan).
Huang Y., Liu J., He J., Hu Z., Tan F., Zhu X., Yuan F, & Jiang Z. (2022). UBIAD1 alleviates ferroptotic neuronal death by enhancing antioxidative capacity by cooperatively restoring impaired mitochondria and Golgi apparatus upon cerebral ischemic/reperfusion insult. Cell & Bioscience, 12, 42.
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4

Lipid Peroxidation Measurement in Rat Cortex

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The ipsilateral cortex of SD rats from each group was used for the LPO measurements. LPO level was detected using the lipid peroxidation assay kit (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China). The brain tissues were digested and then incubated with lipid peroxidation assay kit solution at 45°C for 1 h. After centrifugation, the supernatant was used to determine the optical density (OD) value under a spectrophotometer (Tianpu, Shanghai, China). LPO concentration was calculated based on the protein concentration and OD value according to the manufacturer's instructions.
He J., Liu J., Huang Y., Tang X., Xiao H., Liu Z., Jiang Z., Zeng L., Hu Z, & Lu M. (2021). OM-MSCs Alleviate the Golgi Apparatus Stress Response following Cerebral Ischemia/Reperfusion Injury via the PEDF-PI3K/Akt/mTOR Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2021, 4805040.
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5

Antioxidant Biomarker Quantification in PC12 Cells

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PC12 cells were seeded in 6-well plates at 1 × 106 cells/mL, and the cell supernatants were collected after cell grouping and administration as described above. Intracellular ROS, MDA, and SOD levels were determined using a ROS assay kit, lipid peroxidation assay kit, and SOD assay kit following the manufacturer's instructions (Nanjing Jiancheng).
Wang L, & Zhang L. (2022). Protein Kinase N2 Reduces Hydrogen Peroxide-inducedDamage and Apoptosis in PC12 Cells by AntiOxidative Stress and Activation of the mTOR Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 2483669.
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6

Oxidative Stress Markers in Prostate

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After treatment, SOD (A001-3), CAT (A007-1), and MDA (A003-1) levels in the prostate tissue were determined using the Total SOD Assay Kit, CAT Assay Kit, and Lipid Peroxidation Assay Kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), respectively, according to the manufacturer’s instructions.
Wang H., He L., Liu Z., Xu X., Zhang H., Mao P, & Li M. (2023). Calycosin protects against chronic prostatitis in rats via inhibition of the p38MAPK/NF-κB pathway. Open Medicine, 18(1), 20230770.
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7

Quantifying Lipid Peroxidation in HCC Cells

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The Lipid Peroxidation Assay Kit (Nanjing Jiancheng Institute of Bioengineering, China) was used to measure LPO levels in HCC cells. The results were evaluated at 586 nm using a microplate reader and normalized to protein concentration. All procedures were performed in full accordance with the manufacturer's instructions. Experiments were performed in triplicate.
Chen W., Yang W., Zhang C., Liu T., Zhu J., Wang H., Li T., Jin A., Ding L., Xian J., Tian T., Pan B., Guo W, & Wang B. (2022). Modulation of the p38 MAPK Pathway by Anisomycin Promotes Ferroptosis of Hepatocellular Carcinoma through Phosphorylation of H3S10. Oxidative Medicine and Cellular Longevity, 2022, 6986445.
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8

Intracellular Oxidative Stress Markers

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Intracellular malondialdehyde (MDA) was examined using a lipid peroxidation assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions. The GSH contents were examined using a commercial GSH quantification kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions. All experiments were repeated three times independently.
Liu M., Fan Y., Li D., Han B., Meng Y., Chen F., Liu T., Song Z., Han Y., Huang L., Chang Y., Cao P., Nakai A, & Tan K. (2021). Ferroptosis inducer erastin sensitizes NSCLC cells to celastrol through activation of the ROS–mitochondrial fission–mitophagy axis. Molecular Oncology, 15(8), 2084-2105.
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9

Quantifying Lipid Peroxidation and ROS

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Malondialdehyde (MDA) -an indicator of lipid peroxidation -was determined in the cutaneous tissues and cell lysates. The levels of ROS in keratinocytes were determined to evaluate oxidative stress. The levels of MDA and ROS were measured using a lipid peroxidation assay kit and an ROS kit, respectively (Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China) according to manufacturer's instructions.
Li Y., Ma F., Li H., Song Y., Zhang H., Jiang Z, & Wu H. (2018). Dimethyl fumarate accelerates wound healing under diabetic condition. Journal of molecular endocrinology, 61(4).
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