Disodium dihydrate

Ascorbic acid, collagenase from Clostridium histolyticum, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), elastase from porcine pancreas, (-)-epigallocatechin gallate (EGCG), Folin–Ciocalteu reagent, tyrosinase from mushroom (≥1000 unit/mg solid), ethylenediaminetetraacetic acid, disodium dihydrate (Na₂EDTA*2H₂O), Levodopa (L-DOPA), N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA), N-Succinyl-Ala-Ala-Ala-p-nitroanilide (SANA), Tricine (≥99%; titration) were obtained from Sigma-Aldrich (Steinheim, Germany). Phosphate-buffered saline (PBS) was purchased from Gibco (Carlsbad, CA, USA). Reference substances were from ChromaDex (Irvine, CA, USA). Acetonitrile, formic acid and water for LC analysis were from Merck (Darmstadt, Germany). All others chemicals were of analytical grade and were obtained from Polish Chemical Reagent Company (POCH, Gliwice, Poland).

2,2-diphenyl-1-picrylhydrazyl radical (DPPH^•), 2,2′-azino-bis-(3-ethyl-benzothiazole-6-sulfonic acid) (ABTS^●+), indomethacin, ascorbic acid, ethylene-diaminetetraacetic acid, disodium dihydrate (Na₂EDTA*2H₂O), and Trolox (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic ac-id) were obtained from Sigma-Aldrich (Steinheim, Germany). Reference substances were supplied by ChromaDex (Irvine, CA, USA) while acetonitrile, formic acid, and water were supplied for LC analysis by Merck (Darmstadt, Germany). All other chemicals were of analytical grade and were obtained from the Polish Chemical Reagent Company (POCH, Gliwice, Poland).

Ascorbic acid, collagenase from Clostridium histolyticum, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH^•), 2,2′-azino-bis-(3-ethyl-benzothiazole-6-sulfonic acid) (ABTS^●+), elastase from a porcine pancreas, (-)-epigallocatechin gallate (EGCG), hyaluronidase from the bovine tests, hyaluronic acid sodium salt from a rooster comb, Folin–Ciocalteu reagent, ethylenediaminetetraacetic acid, disodium dihydrate (Na₂EDTA*2H₂O), Tricine (≥99%; titration), N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA), and N-Succinyl-Ala-Ala-Ala-p-nitroanilide (SANA) were obtained from Sigma-Aldrich (Steinheim, Germany). Phosphate-buffered saline (PBS) was purchased from Gibco (Carlsbad, CA, USA). Reference substances were supplied by ChromaDex (Irvine, CA, USA), while acetonitrile, formic acid, and water for LC analysis were supplied by Merck (Darmstadt, Germany). All other chemicals were of analytical grade and were obtained from the Polish Chemical Reagent Company (POCH, Gliwice, Poland).