Curdlan was purchased from Wako Pure Chemical Industries, Itd. (Catalog number: 034-09901, Osaka, Japan). All other chemicals were purchase from Aladdin (Shanghai, China). Dimethylformamide (DMF) was distilled after drying. Dimethyl sulfoxide (DMSO) was dried over 4 Å molecular sieve. Dialysis tube (cut-off molecular weight of 3000; Spectrum Laboratories, Inc.) was used for the purification of Curdlan derivatives. Gel permeation chromatography (GPC) was performed on a Waters HPLC instrument equipped with Ultrahydrogel™ 500 (7.8x300 mm) and Ultrahydrogel™ 250 (7.8x300 mm) columns (Waters) using acetate buffer (pH4.5) as eluent. 13C NMR was recorded on a Brucker 500 NMR spectrometer. Chemical shifts (δ = 0 ppm) were referred to TMS with the residual proton of the deuterated solvent.
Waters hplc instrument
Manufactured by Waters Corporation
Sourced in Japan
The Waters HPLC instrument is a high-performance liquid chromatography (HPLC) system designed for the separation, identification, and quantification of chemical compounds. It utilizes a liquid mobile phase to carry the sample through a stationary phase, enabling the separation of complex mixtures. The instrument is capable of performing various HPLC techniques, such as reverse-phase, normal-phase, and ion-exchange chromatography, to meet the diverse needs of analytical laboratories.
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Lab products found in correlation
3 protocols using waters hplc instrument
1
Curdlan Synthesis and Characterization
2
Spectroscopic Characterization of Organic Compounds
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Melting points were obtained on a Termo Scientific IA9000 series melting point apparatus (Electrothermal, Essex, UK) and were left uncorrected. All NMR spectra were recorded on a Bruker Advance III HD-600 at 600 MHz for 1H-NMR, NOESY, 1H-1H COSY, HSQC, and HMBC, and 150 MHz for 13C-NMR and DEPT in CDCl3, CD3OD, CD3COCD3 and DMSO. Chemical displacements are reported in ppm relative to TMS.
HPLC analysis was performed using a Waters HPLC instrument equipped with Waters 996 UV photodiode array detector (900) set at 280 nm, and employing a packed column SUPELCOSIL LC-F® 25 cm × 4.6 mm, 5 µm, at a flow rate of 0.9 mL/min, and a gradient system of TFA to 0.5%, and H2O (A:B) as the mobile phase with the following solvent ratios: A:B; 100:0 (0–1 min); 95:5 (2–3 min); 70:30 (4–20 min); 50:50 (21–23 min); 20:80 (24–25 min); 0:100 (26–27 min); and, 100:0 (28–30 min). Total running time of samples was 30 min, with a 10 μL injection. The detection wavelength was scanned at 190–400 nm.
For quantification of the isolated compounds, 5 mg in one milliliter of methanol were dissolved in dilution series of 25, 50, 100 and 200 g/mL.
HPLC analysis was performed using a Waters HPLC instrument equipped with Waters 996 UV photodiode array detector (900) set at 280 nm, and employing a packed column SUPELCOSIL LC-F® 25 cm × 4.6 mm, 5 µm, at a flow rate of 0.9 mL/min, and a gradient system of TFA to 0.5%, and H2O (A:B) as the mobile phase with the following solvent ratios: A:B; 100:0 (0–1 min); 95:5 (2–3 min); 70:30 (4–20 min); 50:50 (21–23 min); 20:80 (24–25 min); 0:100 (26–27 min); and, 100:0 (28–30 min). Total running time of samples was 30 min, with a 10 μL injection. The detection wavelength was scanned at 190–400 nm.
For quantification of the isolated compounds, 5 mg in one milliliter of methanol were dissolved in dilution series of 25, 50, 100 and 200 g/mL.
3
Isolation and Purification of 5-Hydroxycyclopenicillone
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5-Hydroxycyclopenicillone was isolated from the fungal strain Trichoderma sp. HPQJ-34 as previous described [12 (link)]. Briefly, a culture medium was filtered under reduced pressure to afford the filtrate and mycelia. The filtrate was further extracted by EtOAc to afford the crude extract, which was subjected to silica gel column chromatography eluted with a petroleum ether-EtOAc step gradient to yield several fractions that were combined based on TLC analysis. The 5-hydroxycyclopenicillone-rich fraction was separated by Sephadex LH-20 gel filtration chromatography, eluting with CH3OH, to yield several sub-fractions. Semi-preparative HPLC was performed on a Waters HPLC instrument equipped with a Waters RID-10A detector and a C18 column (250 mm × 20 mm ID, 5 µm; YMC Co. Ltd. Tokyo, Japan). The subfraction containing 5-hydroxycyclopenicillone was further purified via reverse-phase semi-preparative HPLC with CH3OH/H2O (70:30, v/v) at 2 mL/min to yield 5-hydroxycyclopenicillone. The purity of 5-hydroxycyclopenicillone was greater than 95% as determined by HPLC and 1H-NMR analysis.
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