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Anti atf6

Manufactured by Cosmo Bio
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Sourced in Japan

Anti-ATF6 is a laboratory reagent used to detect and quantify the expression levels of the Activating Transcription Factor 6 (ATF6) protein. ATF6 is a key component of the unfolded protein response (UPR) pathway, which is involved in the cellular response to endoplasmic reticulum (ER) stress. Anti-ATF6 can be used in various research applications, such as Western blotting, immunohistochemistry, and flow cytometry, to study the role of ATF6 in cellular processes and disease states.

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Lab products found in correlation

Anti p erk, by Cell Signaling Technology (2 mentions) Anti xbp1, by BioLegend (2 mentions) Anti phospho eif2α, by Cell Signaling Technology (2 mentions) Anti hsp90, by Santa Cruz Biotechnology (2 mentions) Anti ire1α, by Cell Signaling Technology (2 mentions) Anti atf4, by Santa Cruz Biotechnology (2 mentions) Anti eif2α, by Santa Cruz Biotechnology (2 mentions) Anti tbp, by Abcam (2 mentions)

3 protocols using anti atf6

1

Monitoring UPR Pathway Activation

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Total cell extracts or nuclear extracts were separated by SDS-PAGE and transferred to PVDF membranes. IRE1α phosphorylation was monitored by Phos-tag SDS-PAGE. PERK phosphorylation was monitored by 5% SDS-PAGE. The following antibodies were used for immunoblot analysis: anti-XBP1s (BioLegend, 619502); anti-PERK (Cell signaling, #5683); anti-IRE1α (Cell Signaling, #3294); anti-ATF6 (Cosmo bio, BAM-73-500-EX); anti-ATF4 (Santa Cruz, sc-200); anti-Hsp90 (Santa Cruz, sc-7947), anti-TBP (Abcam, 51841); anti-EIF2α (Santa Cruz, sc-11386); anti-phospho-EIF2α (Cell Signaling, #9721).
Chen X., Iliopoulos D., Zhang Q., Tang Q., Greenblatt M.B., Hatziapostolou M., Lim E., Tam W.L., Ni M., Chen Y., Mai J., Shen H., Hu D.Z., Adoro S., Hu B., Song M., Tan C., Landis M.D., Ferrari M., Shin S.J., Brown M., Chang J.C., Liu X.S, & Glimcher L.H. (2014). XBP1 Promotes Triple Negative Breast Cancer By Controlling the HIF1 α Pathway. Nature, 508(7494), 103-107.
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2

Monitoring UPR Pathway Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cell extracts or nuclear extracts were separated by SDS-PAGE and transferred to PVDF membranes. IRE1α phosphorylation was monitored by Phos-tag SDS-PAGE. PERK phosphorylation was monitored by 5% SDS-PAGE. The following antibodies were used for immunoblot analysis: anti-XBP1s (BioLegend, 619502); anti-PERK (Cell signaling, #5683); anti-IRE1α (Cell Signaling, #3294); anti-ATF6 (Cosmo bio, BAM-73-500-EX); anti-ATF4 (Santa Cruz, sc-200); anti-Hsp90 (Santa Cruz, sc-7947), anti-TBP (Abcam, 51841); anti-EIF2α (Santa Cruz, sc-11386); anti-phospho-EIF2α (Cell Signaling, #9721).
Chen X., Iliopoulos D., Zhang Q., Tang Q., Greenblatt M.B., Hatziapostolou M., Lim E., Tam W.L., Ni M., Chen Y., Mai J., Shen H., Hu D.Z., Adoro S., Hu B., Song M., Tan C., Landis M.D., Ferrari M., Shin S.J., Brown M., Chang J.C., Liu X.S, & Glimcher L.H. (2014). XBP1 Promotes Triple Negative Breast Cancer By Controlling the HIF1 α Pathway. Nature, 508(7494), 103-107.
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3

Immunoblotting and Immunocytochemistry Methods

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Immunoblotting and immunocytochemistry were carried out as described previously (Yoshida et al., 2009) . Anti-ATF6, anti-XBP1, anti-Giantin and anti-GM130 antisera were purchased from Cosmo Bio (Tokyo, Japan) (clone 1-7), Santa Cruz Biotechnology (TX) (sc-7160), COVANCE (Princeton, NJ) (PRB-114C) and Becton, Dickinson and Company (Franklin Lakes, New Jersey) (610823), respectively. Anti -TFE3 antiserum was prepared in the previous study (Taniguchi et al., 2015) .
Sasaki K., Komori R., Taniguchi M., Shimaoka A., Midori S., Yamamoto M., Okuda C., Tanaka R., Sakamoto M., Wakabayashi S, & Yoshida H. (2019). PGSE Is a Novel Enhancer Regulating the Proteoglycan Pathway of the Mammalian Golgi Stress Response. Cell structure and function, 44(1).
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