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23 protocols using sodium borohydride nabh4

1

Synthesis of Bismuth Nanoparticles

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Bismuth nitrate pentahydrate (Bi(NO3)3·5H2O), sodium borohydride (NaBH4), and N,N-dimethylformamide (DMF) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Terephthalic acid (H2BDC) and imidazole were purchased from Aladdin Reagent. The stock solutions of HMIs were prepared by dissolving CdCl2 (Tianjin Bo Di Chemical Industry Co., Ltd., Tianjin, China), PbCl2 (Tianjin Bo Di Chemical Industry Co., Ltd., Tianjin, China), and ZnCl2 (Adamas Reagent Co., Ltd., Shanghai, China) in 1% (v/v) nitric acid. The aqueous solutions were prepared with ultrapure water (≥18 MΩ cm−1) from a Millipore system, and a graphene solution of 5 mg mL−1 was prepared using a modified Hummer’s method (graphite oxide powders, 99.95%, Aladdin Co., Dubai, United Arab Emirates) [25 (link)].
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2

Synthesis of Graphene Oxide-Gold Nanocomposites

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Graphene oxide was purchased from Nanjing Nanotechnology Co., Ltd. SH-β-CD was purchased from Shandong Zhiyuan Biotechnology Company (China). Chloroauric acid tetrahydrate was purchased from Shanghai Jiuyue Chemical Co., Ltd (China). Sodium citrate was purchased from Shanghai Shenbo Chemical Co., Ltd (Shanghai, China). Hexadecyl trimethyl ammonium bromide (CTAB), l(+)-ascorbic acid (L-AA) (>99.0%), sodium borohydride (NaBH4) (>99.0%) and tetrabromobisphenol A were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Other chemicals were of analytical grade and were used without further purification. All the solutions used throughout were prepared with ultra-pure water obtained from a Millipore synergy UV system (resistivity, 18.2 MΩ cm). Before use, all glassware and magnetic stirrers were thoroughly soaked in aqua regia and were then rinsed with copious amounts of deionized water.
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3

Facile Synthesis of Metal Nanoparticles

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Chloroplatinic acid hexahydrate (H2PtCl6·6H2O), copper nitrate trihydrate [Cu(NO3)2·3H2O], cerium(iii) nitrate hexahydrate [Ce(NO3)3·6H2O], sodium hydroxide (NaOH), sodium borohydride (NaBH4), and glycerol were of analytical reagent (A.R.) grade and purchased from Sinopharm Chemical Reagent Co., Ltd. All chemicals were used as received. Deionized water (DI water, Millipore, 18.2 MΩ at 25 °C) were used in all processes.
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4

Nitro-Compound Reduction Protocols

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Hydrogen peroxide (H2O2), nitrobenzene, 4-nitroanisole, 4-nitrotoluene, 4-nitroaniline, 4-chloronitrobenzene, 4-bromonitrobenzene, 4-nitrobenzoic acid, 4-nitrobenzonitrile, 3-bromonitrobenzene, 3-chloronitrobenzene, 3-nitrobenzoic acid, 4-nitrostyrene, 4-nitrophenol (4-NP), thiourea, urea, and sodium borohydride (NaBH4) were purchased from Sinopharm Chemical Reagent Co, Ltd. (Shanghai, China). Potassium tetrachloropalladate (K2PdCl4) was procured from Aladdin Chemistry Co., Ltd. (Shanghai, China). The rest of the chemicals were obtained from Sigma-Aldrich. Deionized water was used for all synthesis and experiments.
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5

Melamine Sponge-Graphene Composite Fabrication

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Melamine sponges were obtained from Ningbo Isida Group Co. Ltd., China, without any surface treatment. Flake graphite with the mesh size of 350 was purchased from Nanjing Xianfeng Nanomaterials Technology Co. Ltd., China. The reducing agent sodium borohydride (NaBH4) was provided by Sinopharm Chemical Reagent Co. Ltd., China. A condensed room-temperature vulcanized silicone rubber (dihydroxy polydimethylsiloxane, RTV-2 type), dimethyl silicone oil (H201-350) and a curing agent were purchased from Xiamen Xinchuang Li Co. Ltd., China.
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6

Synthesis and Characterization of Bredigite-based Biomaterials

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Bredigite (Ca7MgSi4O16, BRT) was purchased from Kunshan Huaqiao New Materials Co., Ltd. Hydroxypropyl methyl cellulose (HPMC) and selenium (Se) were purchased from Aladdin Reagant Co., Ltd. Sodium molybdate (NaMoO4·2H2O) was purchased from Tianjin Fuyu Fine Chemical Co., Ltd. Sodium borohydride (NaBH4) was purchased from Sinopharm Chemical Reagent Co., Ltd. All the chemical reagents were used directly without further purification.
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7

Sensitive DNA Detection Using AgNCs

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Silver nitrate (AgNO3) and sodium borohydride (NaBH4) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All other chemicals (Na2HPO4, NaH2PO4, Mg(AC)2) in this experiment were of analytical grade and used without further treatment. Exonuclease III (Exo III) and the oligonucleotides involved in this experiment were synthesized and purified by Sangon Biotech. Co., Ltd. (Shanghai, China). The detailed information on all DNA sequences is listed in Table 1. Streptavidin magnetic nanobeads (streptavidin-MBs) (size, 1 μm; streptavidin, 40 μg/mg; biotin binding capacity, ≥ 500 pmol of single-stranded 25 bp biotinylated oligonucleotide per mg beads) were provided by New England Biolabs Co., Ltd. (Beijing, China). 20-bp DNA ladder marker and 6 × loading buffer were from Takara Biomedical Technology Co., Ltd. (Dalian, China). All buffer solution for the experiments was prepared with ultrapure water which was obtained from a Millipore water purification system (18.2 MΩ·cm at 25 °C, Milli-Q, Millipore).

DNA sequences used in this work

aThe red sequences in CS represent identification location of target. The blue sequence in SS is the template of AgNCs, and the green part in AS is enhancer of AgNCs. The underlined letters in M1 and M2 indicate the mismatched bases

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8

Synthesis of Cobalt-based Nanoparticles

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Cobalt chloride hexahydrate (CoCl2 · 6H2O), sodium borohydride (NaBH4), sodium citrate dehydrate, and anhydrous ethanol were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd., China. TEOS and 3-aminopropyltriethoxysilane (APS) were purchased from Sigma-Aldrich, St. Louis, MO, USA. All reagents were used as received. Deionized water was distilled by a Milli-Q water purification system (Millipore Corp., Bedford, MA, USA).
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9

Amylin Aggregation Inhibitor Synthesis

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Amylin (1.0 mg package, lot number: 1556842) was purchased from AnaSpec Inc. (San Jose, CA, USA) 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP, 99.8% ACS reagent grade), N-isobutyl-L(D)-cysteine (L(D)-NIBC, 99.9%, Bio reagent grade), ThT was purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydrogen tetrachloroaurate (III) hydrate (HAuCl4·3H2O, 99.999%) was purchased from Acros Organics (Brussels, Belgium). Sodium borohydride (NaBH4, >96%) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Phosphate buffered saline (PBS) plates were purchased from Sangon Biotech (Shanghai, China). The regenerated cellulose dialysis membranes with a molecular weight cut off (Mwco) of 2500D was purchased from Yobios BioTech (Xi’an, China). The ultrafiltration device (Mwco: 30kD) was purchased from Merck Millipore (Darmstadt, Germany). All glass vessels were soaked with aqua regia (volume ratio = 3:1, HCl/HNO3) for 12 h, washed with ultrapure water several times, being dried out, then soaked in chromic acid lotion for 30 min and thoroughly washed by ultrapure water, dried in an oven before use.
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10

Synthesis of Selenium Nanoparticles

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Selenium (reagent powder) was purchased from Shanghai Mei Xing Chemical Co. Ltd CdCl2, ZnCl2, quinoline sulfate and sodium borohydride (NaBH4) were obtained from Sinopharm Chemical Reagent. NAC was purchased from Sigma. All chemicals used were of analytical grade or of the highest purity available. All solutions were prepared using Milli-Q water (Millipore) as the solvent.
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