Mcf 10a

Manufactured by National Centre for Cell Science

Sourced in India

The MCF-10A is a human breast epithelial cell line. It is a non-tumorigenic cell line and is commonly used as a model for normal breast epithelial cells.

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Lab products found in correlation

Mcf 7, by National Centre for Cell Science (4 mentions) Mda mb 231, by National Centre for Cell Science (4 mentions) Methanol, by SD Fine Chemicals (1 mentions) Acetonitrile, by SD Fine Chemicals (1 mentions) Dulbecco s modified eagle media dmem, by HiMedia (1 mentions) Hek 293, by National Centre for Cell Science (1 mentions) Tissue culture plasticware, by BD (1 mentions)

5 protocols using mcf 10a

Cell Line Culture Conditions and Maintenance

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HeLa, MCF-7, MCF 10A, A549, L929, and
B16F10 were bought from National Centre for Cell Sciences (NCCS),
Pune, India. The drug-resistant EMT6/AR1 cell line was bought from
Sigma, St. Louis, MO, USA. HeLa and MCF-7 were maintained using Eagle’s
minimal essential medium. L929 and B16F10 cells were grown in Dulbecco’s
modified Eagle’s medium (DMEM), whereas A549 cells were cultured
in F-12K nutrient medium, supplemented with 10% (v/v) fetal bovine
serum and 1% (v/v) antibiotic–antimycotic solution as described
earlier.^{81 (link)} The EMT6/AR1 cell line was maintained
as described previously.^{44 (link)} MCF 10A was
grown in DMEM and F12K in a 1:1 ratio supplemented with 10% (v/v)
fetal bovine serum, 1% (v/v) antibiotic–antimycotic solution,
epidermal growth factor (20 ng/mL), hydrocortisone (0.5 μg/mL),
and insulin (10 μg/mL).^{82 (link)} All the
cells were grown in optimized conditions in a humidified incubator
in 5% CO₂ at 37 °C (Sanyo, Tokyo, Japan).

Hura N., Sawant A.V., Kumari A., Guchhait S.K, & Panda D. (2018). Combretastatin-Inspired Heterocycles as Antitubulin Anticancer Agents. ACS Omega, 3(8), 9754-9769.

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Metformin-loaded Chitosan Microcapsules

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Apex Laboratories, Chennai, India, kindly gifted Metformin. The 140–220 K OCMC was procured from Indian Sea Food Pvt. Ltd., Kerala India. Crosslinker calcium chloride (CaCl₂) and cryoprotectant trehalose were obtained from Sigma-Aldrich, St. Louis, MO, USA. Acetonitrile and methanol were procured from S.D. Fine Chemicals, Mumbai, India. Dulbecco’s Modified Eagle Media (DMEM) was from Himedia, Mumbai, India. The MCF-7, MDA-MB-231, and MCF-10A cell lines were purchased from the National Centre for Cell Science (NCCS), Pune, India. All other chemicals used were of analytical grade.

De A., Wadhwani A., S.a.u.r.a.j., Roychowdhury P., Kang J.H., Ko Y.T, & Kuppusamy G. (2023). WZB117 Decorated Metformin-Carboxymethyl Chitosan Nanoparticles for Targeting Breast Cancer Metabolism. Polymers, 15(4), 976.

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Cell Culture of Breast Cancer Models

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The human breast carcinoma cell lines (MCF-7, MDA-MB-231, and MDA-MB-415) and non-tumorigenic epithelial cells (MCF-10A) were obtained from National Centre for Cell Sciences (NCCS), Pune, India, and cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% Fetal Bovine Serum (FBS), 2 mM glutamine, and 100 µg/mL penicillin-streptomycin. Cells were cultured in tissue culture flasks at 37 °C with 5% CO₂ in the air. Exponentially growing cells were used for all the experiments.

De A.K., Muthiyan R., Mondal S., Mahanta N., Bhattacharya D., Ponraj P., Muniswamy K., Kundu A., Kundu M.S., Sunder J., Karunakaran D., Bera A.K., Roy S.D, & Malakar D. (2019). A Natural Quinazoline Derivative from Marine Sponge Hyrtios erectus Induces Apoptosis of Breast Cancer Cells via ROS Production and Intrinsic or Extrinsic Apoptosis Pathways. Marine Drugs, 17(12), 658.

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Cell Culture and Maintenance Protocols

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Tissue culture plasticware was purchased from BD Biosciences (CA, USA). MDAMB231, MCF7, MCF10A and HEK 293 were obtained from National Centre for Cell Science (NCCS), Pune. The cells were grown in DMEM supplemented with 2 mM L-glutamine, 100 units/ml of penicillin/streptomycin, and 10% fetal bovine serum (FBS). They were incubated in a humidified 5% CO₂ incubator at 37°C.

Wani K.D., Kadu B.S., Mansara P., Gupta P., Deore A.V., Chikate R.C., Poddar P., Dhole S.D, & Kaul-Ghanekar R. (2014). Synthesis, Characterization and In Vitro Study of Biocompatible Cinnamaldehyde Functionalized Magnetite Nanoparticles (CPGF Nps) For Hyperthermia and Drug Delivery Applications in Breast Cancer. PLoS ONE, 9(9), e107315.

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Culturing Breast Cancer and Normal Cell Lines in Mice

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Human breast cancer cell line MDA-MB-231 and human normal breast cell line MCF-10A were obtained from the National Centre for Cell Science. MDA-MB-231 cells were cultured in RPMI-1640 medium 10% fetal bovine serum (FBS) and maintained in a 5% CO₂ humidified incubator at 37 °C. MCF-10A cells were cultured in GibcoDulbecco’s Modified Eagle Medium: F-12 (DMEM/F-12) 10% fetal bovine serum (FBS) and maintained in a 5% CO₂ humidified incubator at 37 °C.
Female nude mice (Balb/c) were purchased from Shanghai Sipu-BiKAI Laboratory Animal Co., Ltd (Shanghai, China). The mice were housed in a controlled environment with a constant temperature of 26–28 °C and humidity ranging from 40% −60%. Sterile double distilled water and sterile chow were provided as food and water sources for the mice. The experiments were carried out in strict accordance with the regulations for the Management of experimental animals of Nanjing Normal University and with the Guide for the Care and Use of Laboratory Animals.

Hu Y., Wang D., Zhang T., Lei M., Luo Y., Chen Z., Li Y., Duan D., Zhang L, & Zhu Y. (2024). Combined Photosensitive Gene Therapy Effective Against Triple-Negative Breast Cancer in Mice Model. International Journal of Nanomedicine, 19, 1809-1825.

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