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Cobas trigl

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The Cobas TRIGL is a laboratory instrument manufactured by Roche that is used to measure the levels of triglycerides in blood samples. It is a core component of the Cobas product line, designed to provide accurate and reliable triglyceride testing in clinical settings.

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Lab products found in correlation

Cobas cho2i, by Roche (2 mentions) Cho2a, by Roche (2 mentions) Cobas hdlc3, by Roche (2 mentions) Beckman glucose analyzer 2, by Beckman Coulter (1 mentions) Beckman glucose analyser 2, by Beckman Coulter (1 mentions) Rat elisa kits, by Crystal Chem (1 mentions)

4 protocols using cobas trigl

1

Enzymatic Determination of Cholesterol and Triglycerides

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Cholesterol and triglyceride levels were measured in plasma samples of control and HFD rats. The enzymatic method to determine cholesterol level is based on the cleavage of cholesterol esters by cholesterol esterase to yield free cholesterol and fatty acids. Cholesterol oxidase then catalyzed the oxidation of cholesterol to cholest-4-en-3-one and hydrogen peroxidase. In the presence of peroxidase, the hydrogen peroxide formed affected the oxidative coupling of phenol and 4-aminophenazone to form a red quinone-imine dye. The color intensity of the dye formed is directly proportional to the cholesterol concentration and was determined by measuring the increase in absorbance (Cobas CHO2I and CHO2A, Roche Diagnostics, Mannheim, Germany).
The enzymatic triglyceride assay is based on using a lipoprotein lipase for the rapid and complete hydrolysis of triglycerides to glycerol followed by oxidation to dihydroxyacetone phosphate and hydrogen peroxide. The hydrogen peroxide produced then reacted with 4-aminophenazone and 4-chlorophenol under the catalytic action of peroxidase and a red dye was formed. The color intensity of the red dye formed is directly proportional to the triglyceride concentration and was measured photometrically (Cobas TRIGL, Roche Diagnostics, Mannheim, Germany).
van Haare J., Kooi M.E., Vink H., Post M.J., van Teeffelen J.W., Slenter J., Munts C., Cobelens H., Strijkers G.J., Koehn D, & van Bilsen M. (2015). Early impairment of coronary microvascular perfusion capacity in rats on a high fat diet. Cardiovascular Diabetology, 14, 150.
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2

Enzymatic Measurement of Cholesterol and Triglycerides

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Cholesterol and triglyceride levels were measured in plasma samples of HFD rats, metformin- and sulodexide-treated rats. The enzymatic method to determine cholesterol level is based on the cleavage of cholesterol esters by cholesterol esterase to yield free cholesterol and fatty acids. Cholesterol oxidase then catalyzed the oxidation of cholesterol to cholest-4-en-3-one and hydrogen peroxidase. In the presence of peroxidase, the hydrogen peroxide formed affected the oxidative coupling of phenol and 4-aminophenazone to form a red quinone-imine dye. The color intensity of the dye formed is directly proportional to the cholesterol concentration and was determined by measuring the increase in absorbance (Cobas CHO2I and CHO2A, Roche Diagnostics, Mannheim, Germany).
The enzymatic triglycerides assay is based on using a lipoprotein lipase for the rapid and complete hydrolysis of triglycerides to glycerol followed by oxidation to dihydroxyacetone phosphate and hydrogen peroxide. The hydrogen peroxide produced then reacted with 4-aminophenazone and 4-chlorophenol under the catalytic action of peroxidase and a red dye was formed. The color intensity of the red dye formed is directly proportional to the triglyceride concentration and was measured photometrically (Cobas TRIGL, Roche Diagnostics, Mannheim, Germany).
van Haare J., Kooi M.E., van Teeffelen J.W., Vink H., Slenter J., Cobelens H., Strijkers G.J., Koehn D., Post M.J, & van Bilsen M. (2017). Metformin and sulodexide restore cardiac microvascular perfusion capacity in diet-induced obese rats. Cardiovascular Diabetology, 16, 47.
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3

Plasma Glucose and Lipid Measurements

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Plasma glucose concentrations were determined twice by the glucose oxidase method using a Beckman glucose analyzer II (Beckman Instruments, Brea, CA, USA). Total plasma cholesterol, HDL-C, and total plasma triglycerides were measured the cholesterol esterase–cholesterol oxidase–peroxidase reaction (Cobas CHOL2, Roche, Basel, Switzerland), the cholesterol esterase–cholesterol oxidase–peroxidase reaction (Cobas HDLC3, Roche, Basel, Switzerland), and the glycerol phosphate oxidase and peroxidase (Cobas TRIGL, Roche, Basel, Switzerland) enzymatic colorimetric methods, respectively.
del Castillo-Izquierdo Á., Moreno-Navarrete J.M., Latorre J., Arnoriaga-Rodríguez M., Ballanti M., Monteleone G., Alessandro Paoluzi O., Mingrone G., Puig J., Ramos R., Garre-Olmo J., Jové M., Pamplona R., Portero-Otín M., Sol J., Lefebvre P., Staels B., Federici M., Fernández-Real J.M, & Mayneris-Perxachs J. (2022). DPP9 as a Potential Novel Mediator in Gastrointestinal Virus Infection. Antioxidants, 11(11), 2177.
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4

Measuring Hormones and Metabolites in Rat and Human Samples

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For the rat samples, plasma levels of T3 and T4 were measured using rat ELISA kits (Crystal Chem Inc; Downers Grove, IL, USA) (Lopez et al. 2010 (link), Gonzalez et al. 2012 (link), Varela et al. 2012 (link)). For the human samples, serum glucose concentrations were measured in duplicate by the glucose oxidase method using a Beckman Glucose Analyser II (Beckman Instruments; Brea, CA, USA). Roche Hitachi Cobas c711 instrument (Roche) was used to perform HDL cholesterol and total serum triglycerides determinations. HDL cholesterol was quantified by a homogeneous enzymatic colorimetric assay through the cholesterol esterase/cholesterol oxidase/peroxidase reaction (Cobas HDLC3; Roche). Serum fasting triglycerides were measured by an enzymatic, colorimetric method with glycerol phosphate oxidase and peroxidase (Cobas TRIGL; Roche). LDL cholesterol was calculated using the Friedewald formula. Serum free T4 was measured by electrochemiluminescence (Roche Diagnostics) with intra- and inter-assay coefficients of variation less than 5%. Methods have been previously reported (Ortega et al. 2015 (link), Gavalda-Navarro et al. 2016 (link)).
Martínez-Sánchez N., Moreno-Navarrete J.M., Contreras C., Rial-Pensado E., Fernø J., Nogueiras R., Diéguez C., Fernández-Real J.M, & López M. (2016). Thyroid hormones induce browning of white fat. The Journal of Endocrinology, 232(2), 351-362.
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