Pu 1 sc 352x

Manufactured by Santa Cruz Biotechnology

PU.1 (sc-352X) is a lab equipment product offered by Santa Cruz Biotechnology. It is an antibody that specifically targets the PU.1 protein, which is a transcription factor involved in the regulation of gene expression. This product can be used for various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the role of PU.1 in cellular processes.

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Lab products found in correlation

Ab186407, by Abcam (1 mentions) Ab58287, by Abcam (1 mentions) Ab6556, by Abcam (1 mentions) Ezh2 d2c9, by Cell Signaling Technology (1 mentions) P65 sc 372x, by Santa Cruz Biotechnology (1 mentions) H3k4me1 ab8895, by Abcam (1 mentions) H3k4me3, by Merck Group (1 mentions) Ab3594, by Abcam (1 mentions) Ab137055, by Abcam (1 mentions) Ab9050, by Abcam (1 mentions) M2 monoclonal, by Merck Group (1 mentions)

Close spelling variants of this product

Sc-352X

3 protocols using pu 1 sc 352x

Antibody Validation for Western Blotting

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Antibodies were used as following: Histone H3 (WB 1:5000) was a generous gift from Dr. Zhiguo Zhang; anti-DPD (ab6556, WB 1:2000), TS (ab58287, WB 1:2000) and Tubulin (ab186407, WB 1:5000) were purchased from Abcam; H3K27me3 (C36B11, WB 1:5000) and Ezh2 (D2C9, WB 1:2000) were purchased from Cell Signaling Technology; PU.1 (sc-352 X, WB 1:1000) was purchased from Santa Cruz. Primers used for real-time PCR are list in Table S1.

Wu R., Nie Q., Tapper E.E., Jerde C.R., Dunlap G.S., Shrestha S., Elraiyah T.A., Offer S.M, & Diasio R.B. (2016). Histone H3K27 trimethylation modulates 5-fluorouracil resistance by inhibiting PU.1 binding to the DPYD promoter. Cancer research, 76(21), 6362-6373.

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Western Blot and ChIP Antibody Panel

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The following antibodies were used in western blot and ChIP: TRIM33 (3E9, Euromedex for WB (1:1,000); A301-059A, Bethyl for ChIP (1:100)), c-JUN (sc-1694X, Santa Cruz (1:200)), p65 (sc-372X, Santa Cruz (1:200)), RNA Pol II (sc-899X, Santa Cruz (1:200)), H3K4me3 (07-473, Millipore (1:500)), PU.1 (sc-352X, Santa Cruz (1:10,000 for western blot; 1:500 for ChIP)), p300 (sc-585X, Santa Cruz), AcH3 (06-599, Millipore (1:500)), H3K4me1 (ab-8895, Abcam (1:500)), Flag (M2 monoclonal, Sigma Aldrich (1:1,000)), CBP (A-22, sc-369, Santa Cruz (1:1,000)), β-actin (A5441, Sigma Aldrich (1:10,000)).

Ferri F., Parcelier A., Petit V., Gallouet A.S., Lewandowski D., Dalloz M., van den Heuvel A., Kolovos P., Soler E., Squadrito M.L., De Palma M., Davidson I., Rousselet G, & Romeo P.H. (2015). TRIM33 switches off Ifnb1 gene transcription during the late phase of macrophage activation. Nature Communications, 6, 8900.

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Immunoblotting, IP, and ChIP Protocols

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The following antibodies were used in immunoblotting, immunprecipitation and ChIP:
TRIM33 (3E9, Euromedex for WB and IP; A301-059A, Bethyl for ChIP), SPT16 (sc-28734, Santa Cruz), SSRP1 (10D1, Biolegend), PU.1 (sc-352X, Santa Cruz), ATP1B3 (ab137055, Abcam), RNA POLII (sc-9001X, Santa Cruz), H3K4me3 (07-473, Millipore), H3K79me2 (ab3594, Abcam), H3K36me3 (ab9050, Abcam), FLAG (M2 monoclonal, Sigma Aldrich).
Primer sequences for RT-qPCR are available upon request.

Ferri F., Petit V., Barroca V, & Romeo P.H. (2019). Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elements. Epigenetics & Chromatin, 12, 46.

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