500 mhz nmr system

Manufactured by Bruker

The 500 MHz NMR system is a high-performance nuclear magnetic resonance spectrometer designed for analytical applications. It operates at a frequency of 500 MHz, providing high-resolution data for the identification and characterization of chemical compounds.

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500 MHz NMR (28 citations) NMR system (6 citations) AVANCE III 500 MHz NMR system (4 citations) 500 MHz system (3 citations)

2 protocols using 500 mhz nmr system

Quantifying Unreacted Adamantane in PS Particles

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The
supernatant of PS particles after EDC coupling with 1-adamantylamine
hydrochloride was collected and concentrated by rotary evaporation.
The resulting product was resuspended in 1 mL of deuterated water
(D₂O) with 1 mM trimethylsilylpropionic acid (TMSP) as
the internal standard. A Bruker 500 MHz NMR system was used for obtaining
the ¹H NMR spectra. The characteristic Ad peaks at 1.6
ppm in supernatant samples were integrated to the TMSP peak at 0 ppm
to calculate the concentration of unreacted Ad left in each sample.
Ad left within the dispersion of PS particles could then be determined
by the difference between the Ad added to the reaction mixture compared
to the unreacted Ad left in the supernatant.

Willems S.B., Bunschoten A., Wagterveld R.M., van Leeuwen F.W, & Velders A.H. (2019). On-Flow Immobilization of Polystyrene Microspheres on β-Cyclodextrin-Patterned Silica Surfaces through Supramolecular Host–Guest Interactions. ACS Applied Materials & Interfaces, 11(39), 36221-36231.

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Metabolite Quantification by 1H-NMR Spectroscopy

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For the intracellular glucose and glutamine measurements, cells were methanol fixed, and harvested by scraping, then phase separated by the serial addition of chloroform and water, followed by centrifugation. Divalent ions were removed by the addition then removal by centrifugation of Chelex 100. Cell extracts were prepared for ¹H-MRS by freeze drying, followed by resuspension in D₂O and 0.75% TSP (in D₂O) at a 14:1 ratio. For the glucose and glutamine uptake measurements, 1H-MRS were performed on 500 μl of culture media samples (starting media that has not been incubated with the cells and media samples that has been collected just before cell harvest for extractions) with the addition of 50 μl of D2O and 50 μl of 0.75% TSP. ¹H-MR spectra of the extracted and media samples were acquired at 25°C using a pulse-acquired MR sequence with water suppression (1D NOESY presat sequence) in a broad-band-inverse NMR probe on a Bruker 500 MHz NMR system.

Briston T., Stephen J.M., Thomas L.W., Esposito C., Chung Y.L., Syafruddin S.E., Turmaine M., Maddalena L.A., Greef B., Szabadkai G., Maxwell P.H., Vanharanta S, & Ashcroft M. (2018). VHL-Mediated Regulation of CHCHD4 and Mitochondrial Function. Frontiers in Oncology, 8, 388.

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