Mouse monoclonal anti-ubiquitin, HuR, and GFP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse monoclonal anti-α tubulin was from Invitrogen. Mouse monoclonal Anti-βIII Tubulin (2G10), p62/SQSTM1, MAP2 and FMRP was obtained from Millipore (Billerica, MA). Rabbit polyclonal anti-N-terminus FUS was from Bethyl Labs (Montgomery, TX, USA). Anti-TDP-43 and G3BP antibodies were from Proteintech (Chicago, IL, USA). Rabbit polyclonal anti-GFP antibody was from Rockland Immunochemicals Inc. (Gilbertsville, PA, USA). Mouse monoclonal anti-GFP antibody was from Santa Cruz Biotechnology, Inc (Dallas, TX, USA).Mouse Anti-Ataxin-2 was purchased from BD Biosciences (Sparks, MD, USA). Rabbit polyclonal anti-GAPDH antibodies were from Cell Signaling Technology (Beverly, MA, USA). Propidium iodide was purchased from Dojindo (Kumamoto, Japan). Emetine, and Arsenite was purchased from Sigma-Aldrich.
Mouse anti ataxin 2
Manufactured by BD
Sourced in United States
Mouse anti-ataxin-2 is a laboratory reagent used in research applications. It is an antibody that specifically binds to and detects the ataxin-2 protein, which is involved in various cellular processes. The antibody can be used to identify and study the expression and localization of ataxin-2 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti sqstm1, by Novus Biologicals (2 mentions)
Mouse monoclonal anti gfp antibody, by Santa Cruz Biotechnology (1 mentions)
Monoclonal mouse anti α tubulin, by Thermo Fisher Scientific (1 mentions)
Arsenite, by Merck Group (1 mentions)
Mouse monoclonal anti ubiquitin, by Santa Cruz Biotechnology (1 mentions)
Emetine, by Merck Group (1 mentions)
Propidium iodide, by Dojindo Laboratories (1 mentions)
Rabbit polyclonal anti gfp antibody, by Rockland Immunochemicals (1 mentions)
Alexa fluor 594 anti rabbit, by Thermo Fisher Scientific (1 mentions)
Anti mouse alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 anti rabbit, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 anti mouse, by Thermo Fisher Scientific (1 mentions)
Anti g3bp1, by BD (1 mentions)
Rabbit anti ha, by BioLegend (1 mentions)
Mouse anti ubiquitin, by BD (1 mentions)
Mouse anti gfap, by BioLegend (1 mentions)
Protease inhibitor, by Roche (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Nzybradford reagent, by NZYTech (1 mentions)
Rabbit anti lc3b, by Merck Group (1 mentions)
4 protocols using mouse anti ataxin 2
1
Antibody Characterization for Neurodegenerative Research
2
Immunochemical Procedures for Protein Detection
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For immunochemical procedures, the following primary antibodies were used: mouse anti-ataxin-2 (1:1000, ref. 611378, BD Biosciences); mouse anti-ubiquitin (1:1000, ref. 3936S, Cell Signaling) rabbit anti-DARPP-32 (1:1000, ref. AB10518, Merck Millipore); rabbit anti-G3BP1 (1:1000, ref. 07-1801, Millipore); mouse anti-human G3BP1 (1:1000, ref. 611126, BD Biosciences); anti-G3BP1 (1:1000, ref. 05-1938; Sigma-Aldrich); mouse anti-GFAP (1:1000, ref. 644702, BioLegend); rabbit anti-HA (1:1000, ref. Ab9110, Abcam); mouse anti-calbindin D-28K (1:1000, ref. C9848, Sigma-Aldrich); mouse anti-PABP-1 (1:1000, ref. 04-1467, Millipore) and mouse β-Gal (14B7) (1:500, ref. 2372, Cell Signaling Technology). For fluorescence immune procedures, the following antibodies were used: anti-mouse AlexaFluor 647 (1:200, ref. A21235, Invitrogen), anti-mouse AlexaFluor 488 (1:200, ref. A11001, Invitrogen), anti-rabbit AlexaFluor 488 (1:200, ref. A11008, Invitrogen), anti-mouse AlexaFluor 594 (1:200, ref. A11005, Invitrogen) and anti-rabbit AlexaFluor 594 (1:200, ref. A11012, Invitrogen). For light imaging, the following antibodies were used: anti-mouse biotinylated (1:200, ref. BA-9200, Vector Laboratories) and anti-rabbit biotinylated (1:200, ref. BA-1000, Vector Laboratories).
3
Analysis of Ataxin-2 and Autophagy Markers
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Cell extracts were lysed in 10x RIPA solution (Merck Millipore) and or mouse striatal punches were homogenized in a urea/DTT solution, both containing a cocktail of protease inhibitors (Roche pharmaceuticals), followed ultrasound sonication. Protein concentration levels were determined using Pierce™ BCA Protein Assay Kit (Thermo Scientific) for cell lysates and using NZYBradford reagent (Nzytech) for mouse samples. Sixty to eighty protein µg were resolved in SDS-polyacrylamide gels (7.5% and 12%). Followed by protein transfer to PVDF membrane (Merck Millipore) and antibody probing using the following antibodies: mouse anti-ataxin-2 (1:1000, ref. 611378, BD Biosciences); mouse anti-IC2 (polyQ chain) (1:1000, ref. MAB1574, Merck Millipore); rabbit anti-LC3B (1:1000, ref. NB100-2220, Novus); rabbit anti-SQSTM1 (1:1000, ref. 5114S, Cell Signaling); mouse anti-β-actin (1:5000, ref. A5316, Sigma Aldrich); rabbit anti-DARP-32 (1:1000, ref. AB10518, Merck Millipore). Optical densiometric analysis was carried out using Image J software (USA).
4
Immunohistochemistry Antibody Validation
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For immunochemical procedures, the following primary antibodies were used: mouse anti-ataxin-2 (1:1000, ref. 611378, BD Biosciences); rabbit anti-DARP-32 (1:1000, ref. AB10518, Merck Millipore); mouse anti-Iba1/AIF1 (1:1000, ref. MABN92, Merck Millipore); mouse anti-GFAP (1:1000, ref. 644702, BioLegend); rabbit anti-LC3B (1:1000, ref. NB100-2220, Novus); rabbit anti-SQSTM1 (1:1000, ref. 5114S, Cell Signaling); rabbit anti-Cleaved Caspase-3 (Asp175) (1:1000, ref. 9661, Cell Signaling); rabbit anti-Cleaved Caspase-3 (Asp175) (1:1000, ref. 9661, Cell Signaling); rabbit anti-GFAP (1:500, ref. Z0334, Dako, Agilent); rabbit anti-Iba1 (1:1000, ref. 019-19741, FUJIFILM Wako Pure Chemical Corporation) and rabbit anti-NeuN (1:1000, ref. ABN78, Millipore).
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