Six-to-eight-week-old female BALB/c mice were maintained in a pathogen-free area. To generate the chronic asthma model, the mice were intranasally challenged twice a week for 8 weeks with 22 μg of chicken ovalbumin (OVA; Grade V; Sigma Chemical Co., St Louis, MO, USA) and 5 μg fungal protease (protease from Aspergillus melleus; Sigma). In the control group, phosphate-buffered saline was administered. The animals were divided into three groups as follows: sham, a low dose of SAMe (30 mg kg−1; Sigma), and a high dose of SAMe (150 mg kg−1). SAMe was administered daily with feedings for 8 weeks starting at day 0 (Figure 1 ). All mice were killed at day 58, and lung tissue was obtained for histopathological examination. The animal experiments described in this study were approved by our Institutional Animal Care and No Endobronchial Lesion Use Committee.
Chicken ovalbumin ova grade 5
Manufactured by Merck Group
Sourced in United States
Chicken ovalbumin (OVA; Grade V) is a purified protein derived from egg white. It is a widely used laboratory reagent with a well-defined molecular structure and chemical characteristics.
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Lab products found in correlation
2 protocols using chicken ovalbumin ova grade 5
1
Chronic Asthma Model in BALB/c Mice
2
Preparation and Formulation of Quercetin
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Quercetin was purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA) as a preservative-free pure powder. Quercetin was first dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10.0 mM. This solution was then diluted with Airway Epithelial Cell Growth Media (AECG medium; PromoCell GmbH, Heidelberg, Germany) at appropriate concentrations for the experiments; then, the solution was sterilized by passing through 0.2-μm filters and was stored at 4 °C until use. To assess in vivo use, Quercetin was mixed with 5% tragacanth gum solution at a concentration of 7.5 mg/mL [13 (link)]. Chicken ovalbumin (OVA; grade V) and Al(OH)3 (alum) were obtained from Sigma-Aldrich Co. Ltd. as preservative-free pure powders.
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