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Clone p1h12

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The Clone P1H12 is a mouse monoclonal antibody that recognizes a specific antigen on the surface of cells. It can be used for the identification and characterization of this antigen in various applications.

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Lab products found in correlation

Facsdiva v6, by BD (2 mentions) Zombie violet fixable viability kit, by BioLegend (2 mentions) Clone wm59, by BioLegend (2 mentions) Facscanto 2, by BD (2 mentions)

2 protocols using clone p1h12

1

Fetal Pericyte Surface Characterization

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Surface staining of fetal pericytes was performed with CD31-AF488 (1:100 dilution; clone WM59, BioLegend, #303110) and CD146-AF647 (1:100 dilution; clone P1H12, BioLegend, #361014) antibodies. Zombie Violet fixable viability kit (BioLegend, #423113) was used for fixable live/dead staining as per manufacturer instructions. FACS acquisitions was performed on BD FACSCanto II (BD Biosciences), using BD FACSDIVA v 6.0. All FACS data were analyzed using FlowJo v10.0 software.
Chen W., Foo S.S., Hong E., Wu C., Lee W.S., Lee S.A., Evseenko D., Lopes Moreira M.E., García-Sastre A., Cheng G., Nielsen-Saines K., Brasil P., Avvad-Portari E, & Jung J.U. (2021). Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses. Nature microbiology, 6(4), 455-466.
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2

Fetal Pericyte Surface Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Surface staining of fetal pericytes was performed with CD31-AF488 (1:100 dilution; clone WM59, BioLegend, #303110) and CD146-AF647 (1:100 dilution; clone P1H12, BioLegend, #361014) antibodies. Zombie Violet fixable viability kit (BioLegend, #423113) was used for fixable live/dead staining as per manufacturer instructions. FACS acquisitions was performed on BD FACSCanto II (BD Biosciences), using BD FACSDIVA v 6.0. All FACS data were analyzed using FlowJo v10.0 software.
Chen W., Foo S.S., Hong E., Wu C., Lee W.S., Lee S.A., Evseenko D., Lopes Moreira M.E., García-Sastre A., Cheng G., Nielsen-Saines K., Brasil P., Avvad-Portari E, & Jung J.U. (2021). Zika virus NS3 protease induces bone morphogenetic protein-dependent brain calcification in human fetuses. Nature microbiology, 6(4), 455-466.
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