Gradient sds page minigels

Manufactured by Bio-Rad

4–15% gradient SDS–PAGE minigels are laboratory equipment used for protein separation and analysis through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These gels feature a linear gradient of 4% to 15% polyacrylamide concentration, allowing for the effective separation of a wide range of protein molecular weights.

Automatically generated - may contain errors

Lab products found in correlation

Bradford method, by Bio-Rad (2 mentions) Clone h 3, by Santa Cruz Biotechnology (2 mentions) Clone c 10, by Santa Cruz Biotechnology (2 mentions) Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Hrp conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

SDS-PAGE (902 citations) 4–20% gradient SDS-PAGE (55 citations) Minigel (22 citations) 7.5% SDS-PAGE minigels (16 citations) 12% SDS minigel (2 citations)

2 protocols using gradient sds page minigels

Annexin Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Atg16L⁺ FACS-sorted vesicles were lysed in 1% NP40, 50 mM Tris/HCl and 150 mM NaCl lysis buffer. Protein concentration was determined by the Bradford method (Bio-Rad Laboratories). Total proteins (20 μg) were resolved on 4–15% gradient SDS–PAGE minigels (Bio-Rad). Total cell lysate and BMDC cytosol were run as positive controls. After transfer to nitrocellulose membranes and blocking in PBS with 0.1% Tween 20 and 5% non-fat milk, the membranes were incubated with anti-mouse annexin A2 (1:4,000, Clone C-10 Santa Cruz Biotechnology) or anti-mouse annexin A5 (1:200, Clone H-3, Santa Cruz Biotechnology), followed by horse radish peroxidase-conjugated secondary antibody (Jackson ImmunoResearch). Proteins were visualized by chemiluminescence (Supersignal, WB kit, Thermo Scientific).

Morozova K., Sidhar S., Zolla V., Clement C.C., Scharf B., Verzani Z., Diaz A., Larocca J.N., Hajjar K.A., Cuervo A.M, & Santambrogio L. (2015). Annexin A2 promotes phagophore assembly by enhancing Atg16L+ vesicle biogenesis and homotypic fusion. Nature Communications, 6, 5856.

+ Open protocol

+ Expand

Annexin A2 and A5 Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Atg16L⁺ FACS-sorted vesicles were lysed in 1% NP40, 50 mM Tris/HCl, and 150 mM NaCl lysis buffer. Protein concentration was determined by the Bradford method (Bio-Rad Laboratories). Total proteins (20 μg) were resolved on 4–15% gradient SDS-PAGE minigels (BioRad). Total cell lysate and BMDC cytosol were run as positive controls. After transfer to nitrocellulose membranes and blocking in PBS with 0.1% Tween 20 and 5% non-fat milk, the membranes were incubated with anti-mouse annexin A2 (1:4000, Clone C-10 Santa Cruz) or anti-mouse annexin A5 (1:200, Clone H-3, Santa Cruz), followed by HRP-conjugated secondary antibody (Jackson ImmunoResearch). Proteins were visualized by chemiluminescence (Supersignal, WB kit, Thermo Scientific).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!