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Ab264375

Manufactured by Abcam
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Ab264375 is a laboratory equipment product offered by Abcam. It serves as a general-purpose instrument used for various scientific applications. The core function of this product is to facilitate specific tasks or procedures in a research or laboratory setting. However, a detailed and unbiased description of its features and intended use is not available.

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Lab products found in correlation

Ab128175, by Abcam (1 mentions) Ab243880, by Abcam (1 mentions) Ab221795, by Abcam (1 mentions) Ab208577, by Abcam (1 mentions) Ab229700, by Abcam (1 mentions) Magna rip kit, by Merck Group (1 mentions)

2 protocols using ab264375

1

m6A Regulators Profiling Protocol

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RIP assay was carried out as described previously.33 Antibodies against m6A (ab208577, Abcam), METTL3 (ab221795, Abcam), YTHDC1 (ab264375, Abcam), IGF2BP1 (ab229700, Abcam), IGF2BP2 (ab128175, Abcam), IGF2BP3 (ab250015, Abcam) and FUS (ab243880, Abcam) were used.
Chen Z., Ruan W., Guo C., Chen K., Li L., Tian J., Hu Z., Peng D, & Zeng X. (2023). Non‐SMC condensin I complex subunit H participates in anti‐programmed cell death‐1 resistance of clear cell renal cell carcinomas. Cell Proliferation, 56(7), e13400.
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2

YTHDC1 RNA Interactome Analysis

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The Millipore Magna RIP Kit (Millipore, Darmstadt, Germany) was used to conduct the RIP assay following the directions provided by the manufacturer. Briefly, the RIP lysis buffer, which contains protease and RNase inhibitor, was used to lyse the SKOV3 and A2780 cells. The cell lysate supernatant was then subjected to overnight incubation at 4 °C with anti-YTHDC1 (ab264375, Abcam) antibody-conjugated beads. The binding complexes underwent thorough washing, elution, purification to extract RNAs, and qRT-PCR analysis. The collection of RNAs was normalized to IgG. Additionally, LC-BIO (Hangzhou, China) carried out the high-throughput sequencing.
Wang X., Chen Q., Bing Z., Zhou S., Xu Z., Hou Y., Zhao Y., Zhao S, & Wang T. (2023). Low expression of m6A reader YTHDC1 promotes progression of ovarian cancer via PIK3R1/STAT3/GANAB axis. International Journal of Biological Sciences, 19(14), 4672-4688.
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