Nur77 12.14

Manufactured by Thermo Fisher Scientific

The Nur77 (12.14) is a laboratory equipment product offered by Thermo Fisher Scientific. It serves as a tool for researchers and scientists. The core function of this product is to facilitate specific applications within the laboratory setting. However, a detailed description of its intended use cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Tcrβ h57 597, by BD (1 mentions) Cd62l mel 14, by BD (1 mentions) Facscanto 2 system, by BD (1 mentions) Cd3 145 2c11, by Cytek Biosciences (1 mentions) Foxp3 3g3, by Cytek Biosciences (1 mentions) Annexin 5 fitc apoptosis detection kit, by Immunostep (1 mentions) Sytox blue, by Thermo Fisher Scientific (1 mentions) Fjk 16, by Thermo Fisher Scientific (1 mentions) Helios 22f6, by BioLegend (1 mentions) Ctla 4 uc10 4b9, by BioLegend (1 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Nur77 (6 citations) Anti-NUR77 conjugated to PE (clone 12.14) (3 citations) Nur77-PE (clone 12.14), (2 citations)

2 protocols using nur77 12.14

Multiparametric Flow Cytometry Staining

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Cell staining was done with fluorescently labeled mAbs to CD5 (53–7.3), CD4 (RM4-5), CD8α (53–6.7), CD24 (M1/69), TCR-Vα2 (B20.1TCR-Vβ6 (RR4-7), TCRγ (GL-3), and TCRβ (H57-597) were purchased from BD; CD62L (MEL-14), CD44 (IM7), CD69 (H1.2F3), CD3 (145.2C11), and FoxP3 (3G3) were obtained from TONBO Bioscience; CD25 (PC61.5), CD19 (6D5), CD161 (PK136), and CD6 (OX129) were purchased from BioLegend; and Nur77 (12.14) was obtained from eBioscience; all were used according to the manufacturer’s instructions. FACS analyses were done on a LSRII 564 or FACS Canto II system (BD) using FlowJo software (TreeStar). Cell viability was evaluated either with SYTOX Blue (Life Technologies) or 633- or 405-nm Viability dyes (Life Technologies). Apoptotic cells were detected by using Annexin V-FITC apoptosis detection kit (Immunostep).

Orta-Mascaró M., Consuegra-Fernández M., Carreras E., Roncagalli R., Carreras-Sureda A., Alvarez P., Girard L., Simões I., Martínez-Florensa M., Aranda F., Merino R., Martínez V.G., Vicente R., Merino J., Sarukhan A., Malissen M., Malissen B, & Lozano F. (2016). CD6 modulates thymocyte selection and peripheral T cell homeostasis. The Journal of Experimental Medicine, 213(8), 1387-1397.

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T Cell Phenotyping by Flow Cytometry

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For surface staining, fluorophore-conjugated monoclonal Abs (mAbs) specific for CD3ε(17A2), TCRβ(H57–597), CD4(GK1.5 or RM4–5), PD-1 (RMP1–30), GITR (DTA-1), and CTLA-4 (UC10–4B9) were obtained from BioLegend. The mAb recognizing CXCR5 (2G8) was from BD Pharmingen. The mAb recognizing TCR Vβ6(RR4–7) was from eBioscience. For intranuclear staining, buffers from a Foxp3 Staining Buffer Set (eBioscience) were used to stain with Abs recognizing Foxp3 (FJK-16s, eBioscience), Helios (22F6, Biolegend), and/or Nur77 (12.14, eBioscience). Cells were run on an LSRII (BD Biosciences), and analyses were performed with FlowJo (TreeStar) software.

Confer D.L., Vercellotti G.M., Kotasek D., Goodman J.L., Ochoa A, & Jacob H.S. (1990). Herpes simplex virus-infected cells disarm killer lymphocytes.. Proceedings of the National Academy of Sciences of the United States of America, 87(9), 3609-3613.

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