The catalase assay and glutathione peroxidase assay kits, acquired from Sigma-Aldrich, were employed to assess the intracellular activities of catalase and glutathione peroxidase, respectively, according to the manufacturer's instructions. On the other hand, the intracellular activities of peroxiredoxins were evaluated following a published protocol [25 ]. To ensure the veracity of our enzyme activity assessments, we incorporated affirmative benchmarks to validate the efficacy of the employed methodology. The catalase assay and glutathione peroxidase assay kits, procured from Sigma-Aldrich, were equipped with pre-included reagents serving as positive controls. Moreover, for the evaluation of peroxiredoxin activity, we acquired purified and functionally active peroxiredoxin from MyBioSource.com. Conversely, the negative controls exclusively consisted of reagents devoid of purified enzymes or cellular extracts.
Lei F.J., Chiang J.Y., Chang H.J., Chen D.C., Wang H.L., Yang H.A., Wei K.Y., Huang Y., Wang C.C., Wei S.T, & Hsieh C.H. (2023). Cellular and exosomal GPx1 are essential for controlling hydrogen peroxide balance and alleviating oxidative stress in hypoxic glioblastoma. Redox Biology, 65, 102831.
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